31 research outputs found
Functional Brain Imaging with Multi-Objective Multi-Modal Evolutionary Optimization
Functional brain imaging is a source of spatio-temporal data mining problems.
A new framework hybridizing multi-objective and multi-modal optimization is
proposed to formalize these data mining problems, and addressed through
Evolutionary Computation (EC). The merits of EC for spatio-temporal data mining
are demonstrated as the approach facilitates the modelling of the experts'
requirements, and flexibly accommodates their changing goals
Molecular differential diagnosis of follicular thyroid carcinoma and adenoma based on gene expression profiling by using formalin-fixed paraffin-embedded tissues
Analytical Performance Verification of a Molecular Diagnostic for Cytology-Indeterminate Thyroid Nodules
Thyroid nodules with indeterminate cytology: molecular imaging with 99mTc-methoxyisobutylisonitrile (MIBI) is more cost-effective than the Afirma® gene expression classifier
A Customized Class of Functions for Modeling and Clustering Gene Expression Profiles in Embryonic Stem Cells
Identification of hair cycle-associated genes from time-course gene expression profile data by using replicate variance
The hair-growth cycle is an example of a cyclic process that is well characterized morphologically but understood incompletely at the molecular level. As an initial step in discovering regulators in hair-follicle morphogenesis and cycling, we used DNA microarrays to profile mRNA expression in mouse back skin from eight representative time points. We developed a statistical algorithm to identify the set of genes expressed within skin that are associated specifically with the hair-growth cycle. The methodology takes advantage of higher replicate variance during asynchronous hair cycles in comparison with synchronous cycles. More than one-third of genes with detectable skin expression showed hair-cycle-related changes in expression, suggesting that many more genes may be associated with the hair-growth cycle than have been identified in the literature. By using a probabilistic clustering algorithm for replicated measurements, these genes were grouped into 30 time-course profile clusters, which fall into four major classes. Distinct genetic pathways were characteristic for the different time-course profile clusters, providing insights into the regulation of hair-follicle cycling and suggesting that this approach is useful for identifying hair follicle regulators. In addition to revealing known hair-related genes, we identified genes that were not previously known to be hair cycle-associated and confirmed their temporal and spatial expression patterns during the hair-growth cycle by quantitative real-time PCR and in situ hybridization. The same computational approach should be generally useful for identifying genes associated with cyclic processes from complex tissues