Proteome Serological Determination of Tumor-Associated Antigens in Melanoma

Proteome serology may complement expression library-based approaches as strategy utilizing the patients' immune responses for the identification pathogenesis factors and potential targets for therapy and markers for diagnosis. Melanoma is a relatively immunogenic tumor and antigens recognized by melanoma-specific T cells have been extensively studied. The specificities of antibody responses to this malignancy have been analyzed to some extent by molecular genetic but not proteomics approaches. We screened sera of 94 melanoma patients for anti-melanoma reactivity and detected seropositivity in two-thirds of the patients with 2–6 antigens per case detected by 1D and an average of 2.3 per case by 2D Western blot analysis. For identification, antigen spots in Western blots were aligned with proteins in 2-DE and analyzed by mass spectrometry. 18 antigens were identified, 17 of which for the first time for melanoma. One of these antigens, galectin-3, has been related to various oncogenic processes including metastasis formation and invasiveness. Similarly, enolase has been found deregulated in different cancers. With at least 2 of 18 identified proteins implicated in oncogenic processes, the work confirms the potential of proteome-based antigen discovery to identify pathologically relevant proteins

Ferruginous Pygmy-Owls: A New Host for Protocalliphora sialia and Hesperocimex sonorensis in Arizona

Volume: 117Start Page: 185End Page: 18

[catalog] Catalogus van twee belangrijke verzamelingen boeken, in verschillende vakken van wetenschap en kunst, benevens eenige landkaarten, schilderijen, teekeningen, prentwerken, prenten, portretten, autographen, penningen, mineralen en andere voorwerpen, waarvan de eerste verzameling is nagelaten door den weleerwaarden heer C. D. Usener ... en de tweede... door ... Mr. J. P. Noiret De Bruyn ... en ... Dr. P. A. Fromm...

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