Planar cell polarity genes Frizzled3a, Vangl2, and Scribble are required for spinal commissural axon guidance

Background A fundamental feature of early nervous system development is the guidance of axonal projections to their targets in order to assemble neural circuits that control behavior. Spinal commissural neurons are an attractive model to investigate the multiple guidance cues that control growth cone navigation both pre- and post-midline crossing, as well as along both the dorsal–ventral (D–V) and anterior–posterior (A–P) axes. Accumulating evidence suggests that guidance of spinal commissural axons along the A–P axis is dependent on components of the planar cell polarity (PCP) signaling pathway. In the zebrafish, the earliest born spinal commissural neuron to navigate the midline and turn rostrally is termed commissural primary ascending (CoPA). Unlike mammalian systems, CoPA axons cross the midline as a single axon and allow an analysis of the role of PCP components in anterior pathfinding in single pioneering axons. Results Here, we establish CoPA cells in the zebrafish spinal cord as a model system for investigating the molecular function of planar cell polarity signaling in axon guidance. Using mutant analysis, we show that the functions of Fzd3a and Vangl2 in the anterior turning of commissural axons are evolutionarily conserved in teleosts. We extend our findings to reveal a role for the PCP gene scribble in the anterior guidance of CoPA axons. Analysis of single CoPA axons reveals that these commissural axons become responsive to PCP-dependent anterior guidance cues even prior to midline crossing. When midline crossing is prevented by dcc gene knockdown, ipsilateral CoPA axons still extend axons anteriorly in response to A–P guidance cues. We show that this ipsilateral anterior pathfinding that occurs in the absence of midline crossing is dependent on PCP signaling. Conclusion Our results demonstrate that anterior guidance decisions by CoPA axons are dependent on the function of planar cell polarity genes both prior to and after midline crossing

A large scale extinction map of the Galactic Anticenter from 2MASS

We present a 127deg x 63deg extinction map of the Anticenter of the Galaxy, based on and colour excess maps from 2MASS. This 8001 square degree map with a resolution of 4 arcminutes is provided as online material. The colour excess ratio / is used to determine the power law index of the reddening law (\beta) for individual regions contained in the area (e.g. Orion, Perseus, Taurus, Auriga, Monoceros, Camelopardalis, Cassiopeia). On average we find a dominant value of \beta=1.8+-0.2 for the individual clouds, in agreement with the canonical value for the interstellar medium. We also show that there is an internal scatter of \beta values in these regions, and that in some areas more than one dominant \beta value is present. This indicates large scale variations in the dust properties. The analysis of the A_V values within individual regions shows a change in the slope of the column density distribution with distance. This can either be attributed to a change in the governing physical processes in molecular clouds on spatial scales of about 1pc or an A_V dilution with distance in our map.Comment: 18 pages, 29 Figures, 1 Table, Accepted for publication by MNRAS, A version with higher resolution figures can be found at http://astro.kent.ac.uk/~df

A maximum spreading speed for magnetopause reconnection

Past observations and numerical modeling find magnetic reconnection to initiate at a localized region and then spread along a current sheet. The rate of spreading has been proposed to be controlled by a number of mechanisms based on the properties within the boundary. At the Earth's magnetopause the spreading speed is also limited by the speed at which a shocked solar wind front can move along the magnetopause boundary. The speed at which a purely north to south rotational discontinuity propagates through the magnetosheath and contacts the magnetopause is measured here using the Block‐Adaptive‐Tree Solar Wind Roe‐Type Upwind Scheme global magnetohydrodynamics model. The propagation speed along the magnetopause is fastest near the nose of the magnetopause and decreases with distance from the subsolar point. The average propagation speed along the dayside magnetopause is 847 km/s. This is significantly larger than observed rates of reconnection spreading at the magnetopause of 30–40 km/s indicating that, for the observed conditions, the speed of front propagation along the magnetopause does not limit or control the spreading rate of reconnection.Published versio

Overcoming the false-minima problem in direct methods: Structure determination of the packaging enzyme P4 from bacteriophage φ13

The problems encountered during the phasing and structure determination of the packaging enzyme P4 from bacteriophage φ13 using the anomalous signal from selenium in a single-wavelength anomalous dispersion experiment (SAD) are described. The oligomeric state of P4 in the virus is a hexamer (with sixfold rotational symmetry) and it crystallizes in space group C2, with four hexamers in the crystallographic asymmetric unit. Current state-of-the-art ab initio phasing software yielded solutions consisting of 96 atoms arranged as sixfold symmetric clusters of Se atoms. However, although these solutions showed high correlation coefficients indicative that the substructure had been solved, the resulting phases produced uninterpretable electron-density maps. Only after further analysis were correct solutions found (also of 96 atoms), leading to the eventual identification of the positions of 120 Se atoms. Here, it is demonstrated how the difficulties in finding a correct phase solution arise from an intricate false-minima problem. © 2005 International Union of Crystallography - all rights reserved

Attitude determination of the spin-stabilized Project Scanner spacecraft

Attitude determination of spin-stabilized spacecraft using star mapping techniqu

Evaluation of Stress Corrosion Cracking Susceptibility Using Fracture Mechanics Techniques, Part 1

Stress corrosion cracking (SSC) tests were performed on 13 aluminum alloys, 13 precipitation hardening stainless steels, and two titanium 6Al-4V alloy forgings to compare fracture mechanics techniques with the conventional smooth specimen procedures. Commercially fabricated plate and rolled or forged bars 2 to 2.5-in. thick were tested. Exposures were conducted outdoors in a seacoast atmosphere and in an inland industrial atmosphere to relate the accelerated tests with service type environments. With the fracture mechanics technique tests were made chiefly on bolt loaded fatigue precracked compact tension specimens of the type used for plane-strain fracture toughness tests. Additional tests of the aluminum alloy were performed on ring loaded compact tension specimens and on bolt loaded double cantilever beams. For the smooth specimen procedure 0.125-in. dia. tensile specimens were loaded axially in constant deformation type frames. For both aluminum and steel alloys comparative SCC growth rates obtained from tests of precracked specimens provide an additional useful characterization of the SCC behavior of an alloy

Beneficial modulation of the gut microbiota

peer-reviewedThe human gut microbiota comprises approximately 100 trillion microbial cells and has a significant effect on many aspects of human physiology including metabolism, nutrient absorption and immune function. Disruption of this population has been implicated in many conditions and diseases, including examples such as obesity, inflammatory bowel disease and colorectal cancer that are highlighted in this review. A logical extension of these observations suggests that the manipulation of the gut microbiota can be employed to prevent or treat these conditions. Thus, here we highlight a variety of options, including the use of changes in diet (including the use of prebiotics), antimicrobial-based intervention, probiotics and faecal microbiota transplantation, and discuss their relative merits with respect to modulating the intestinal community in a beneficial way.C.J.W, C.M.G. and P.D.C are supported by a SFI PI award “Obesibiotics” (11/PI/1137

Collision of High Frequency Plane Gravitational and Electromagnetic Waves

We study the head-on collision of linearly polarized, high frequency plane gravitational waves and their electromagnetic counterparts in the Einstein-Maxwell theory. The post-collision space-times are obtained by solving the vacuum Einstein-Maxwell field equations in the geometrical optics approximation. The head-on collisions of all possible pairs of these systems of waves is described and the results are then generalised to non-linearly polarized waves which exhibit the maximum two degrees of freedom of polarization.Comment: Latex file, 17 pages, accepted for publication in International Journal of Modern Physics