Large emissions from floodplain trees close the Amazon methane budget

Wetlands are the largest global source of atmospheric methane (CH4), a potent greenhouse gas. However, methane emission inventories from the Amazon floodplain, the largest natural geographic source of CH4 in the tropics, consistently underestimate the atmospheric burden of CH4 determined via remote sensing and inversion modelling, pointing to a major gap in our understanding of the contribution of these ecosystems to CH4 emissions. Here we report CH4 fluxes from the stems of 2,357 individual Amazonian floodplain trees from 13 locations across the central Amazon basin. We find that escape of soil gas through wetland trees is the dominant source of regional CH4 emissions. Methane fluxes from Amazon tree stems were up to 200 times larger than emissions reported for temperate wet forests6 and tropical peat swamp forests, representing the largest non-ebullitive wetland fluxes observed. Emissions from trees had an average stable carbon isotope value (δ13C) of −66.2 ± 6.4 per mil, consistent with a soil biogenic origin. We estimate that floodplain trees emit 15.1 ± 1.8 to 21.2 ± 2.5 teragrams of CH4 a year, in addition to the 20.5 ± 5.3 teragrams a year emitted regionally from other sources. Furthermore, we provide a ‘top-down’ regional estimate of CH4 emissions of 42.7 ± 5.6 teragrams of CH4 a year for the Amazon basin, based on regular vertical lower-troposphere CH4 profiles covering the period 2010–2013. We find close agreement between our ‘top-down’ and combined ‘bottom-up’ estimates, indicating that large CH4 emissions from trees adapted to permanent or seasonal inundation can account for the emission source that is required to close the Amazon CH4 budget. Our findings demonstrate the importance of tree stem surfaces in mediating approximately half of all wetland CH4 emissions in the Amazon floodplain, a region that represents up to one-third of the global wetland CH4 source when trees are combined with other emission sources

Prostaglandin E2 Prevents Hyperosmolar-Induced Human Mast Cell Activation through Prostanoid Receptors EP2 and EP4

Background: Mast cells play a critical role in allergic and inflammatory diseases, including exercise-induced bronchoconstriction (EIB) in asthma. The mechanism underlying EIB is probably related to increased airway fluid osmolarity that activates mast cells to the release inflammatory mediators. These mediators then act on bronchial smooth muscle to cause bronchoconstriction. In parallel, protective substances such as prostaglandin E2 (PGE2) are probably also released and could explain the refractory period observed in patients with EIB. Objective: This study aimed to evaluate the protective effect of PGE2 on osmotically activated mast cells, as a model of exercise-induced bronchoconstriction. Methods: We used LAD2, HMC-1, CD34-positive, and human lung mast cell lines. Cells underwent a mannitol challenge, and the effects of PGE2 and prostanoid receptor (EP) antagonists for EP1-4 were assayed on the activated mast cells. Beta-hexosaminidase release, protein phosphorylation, and calcium mobilization were assessed. Results: Mannitol both induced mast cell degranulation and activated phosphatidyl inositide 3-kinase and mitogen-activated protein kinase (MAPK) pathways, thereby causing de novo eicosanoid and cytokine synthesis. The addition of PGE2 significantly reduced mannitol-induced degranulation through EP2 and EP4 receptors, as measured by beta-hexosaminidase release, and consequently calcium influx. Extracellular-signal-regulated kinase 1/2, c-Jun N-terminal kinase, and p38 phosphorylation were diminished when compared with mannitol activation alone. Conclusions:Our data show a protective role for the PGE2 receptors EP2 and EP4 following osmotic changes, through the reduction of human mast cell activity caused by calcium influx impairment and MAP kinase inhibition

Isotopic techniques to measure N2O, N2 and their sources

GHG emissions are usually the result of several simultaneous processes. Furthermore, some gases such as N2 are very difficult to quantify and require special techniques. Therefore, in this chapter, the focus is on stable isotope methods. Both natural abundance techniques and enrichment techniques are used. Especially in the last decade, a number of methodological advances have been made. Thus, this chapter provides an overview and description of a number of current state-of-theart techniques, especially techniques using the stable isotope 15N. Basic principles and recent advances of the 15N gas flux method are presented to quantify N2 fluxes, but also the latest isotopologue and isotopomer methods to identify pathways for N2O production. The second part of the chapter is devoted to 15N tracing techniques, the theoretical background and recent methodological advances. A range of different methods is presented from analytical to numerical tools to identify and quantify pathway-specific N2O emissions. While this chapter is chiefly concerned with gaseous N emissions, a lot of the techniques can also be applied to other gases such as methane (CH4), as outlined in Sect. 5.3

Historic carbon burial spike in an Amazon floodplain lake linked to riparian deforestation near Santarém, Brazil

Forests along the Amazon Basin produce significant quantities of organic material, a portion of which is deposited in floodplain lakes. Deforestation in the watershed may then have potentially important effects on the carbon fluxes. In this study, a sediment core was extracted from an Amazon floodplain lake to examine the relationship between carbon burial and changing land cover and land use. Historical records from the 1930s and satellite data from the 1970s were used to calculate deforestation rates between 1930 to 1970 and 1970 to 2010 in four zones with different distances from the margins of the lake and its tributaries (100, 500, 1000 and 6000 m buffers). A sediment accumulation rate of  ∼ 4 mm yr−1 for the previous  ∼ 120 years was determined from the 240+239Pu signatures and the excess 210Pb method. The carbon burial rates ranged between 85 and 298 g C m−2 yr−1, with pulses of high carbon burial in the 1950s, originating from the forest vegetation as indicated by δ13C and δ15N signatures. Our results revealed a potentially important spatial dependence of the organic carbon (OC) burial in Amazon lacustrine sediments in relation to deforestation rates in the catchment. These deforestation rates were more intense in the riparian vegetation (100 m buffer) during the period 1930 to 1970 and the larger open water areas (500, 1000 and 6000 m buffer) during 1970 to 2010. The continued removal of vegetation from the interior of the forest was not related to the peak of OC burial in the lake, but only the riparian deforestation which peaked during the 1950s. Therefore, this supports the conservation priority of riparian forests as an important management practice for Amazon flooded areas. Our findings suggest the importance of abrupt and temporary events in which some of the biomass released by deforestation, especially restricted to areas along open water edges, might reach the depositional environments in the floodplain of the Amazon Basin

Estudio diacrónico de la dieta de los pobladores antiguos de Terrinches (Ciudad Real) a partir del análisis de isótopos estables sobre restos óseos humanos

Este trabajo presenta un estudio diacrónico llevado a cabo para conocer la dieta de los pobladores antiguos del actual término municipal de Terrinches (Ciudad Real) a partir del análisis de isótopos estables del carbono y del nitrógeno sobre restos óseos humanos encontrados en los yacimientos arqueológicos de Castillejo del Bonete (Calcolítico y Edad del Bronce), La Ontavia (Altomedieval) y la Ermita de Nuestra Señora de Luciana (Edad Moderna). Los resultados muestran que, aunque la dieta de estas tres poblaciones estaba basada en recursos terrestres C3, existen algunas diferencias tanto entre periodos históricos como entre individuos de un mismo momento que podrían deberse a dispares procedencias geográficas. This work presents a diachronic dietary study done on the ancient inhabitants of Terrinches (Ciudad Real, Spain). Carbon and nitrogen stable isotope analysis on human bone collagen were carried out on the archaeological sites of Castillejo del Bonete (Chalcolithic and Bronze Age), Ontavia (Early Medieval) and Ermita de Nuestra Señora de Luciana (Modern Age). The results show that the diet of these three populations was based on terrestrial C3 resources, but existing some slight differences between time periods and amongst contemporary individuals that could be result of diverse geographic provenance