Power-law fluctuations in phase-separated lipid membranes

URL:http://link.aps.org/doi/10.1103/PhysRevE.60.7354 DOI:10.1103/PhysRevE.60.7354The spatial structure of three binary lipid mixtures, prepared as multilamellar vesicles, was studied by small-angle neutron scattering. In the fluid-gel coexistence region, large- scale concentration fluctuations appear which scatter like surface fractals for small acyl- chain mismatch and like mass fractals for large mismatch over about one decade of length. The transition is highly discontinuous: The fractal dimension of the boundary between the gel and fluid drops from 2.7 to 1.7, the gel fraction in the fluctuations drops from about 0.5 to 0.07, and the gel domains interlamellar correlation drops from strong to weak. We interpret the fluctuations as long-lived descendants of the incipient two-phase equilibrium state and the transition as due to changes in the gel rigidity and phase diagram.We gratefully acknowledge financial support from the Deutsch Forschungsgemeinschaft and the Petroleum Research Fund, administered by the American Chemical Society

Monoolein Lipid Phases as Incorporation and Enrichment Materials for Membrane Protein Crystallization

The crystallization of membrane proteins in amphiphile-rich materials such as lipidic cubic phases is an established methodology in many structural biology laboratories. The standard procedure employed with this methodology requires the generation of a highly viscous lipidic material by mixing lipid, for instance monoolein, with a solution of the detergent solubilized membrane protein. This preparation is often carried out with specialized mixing tools that allow handling of the highly viscous materials while minimizing dead volume to save precious membrane protein sample. The processes that occur during the initial mixing of the lipid with the membrane protein are not well understood. Here we show that the formation of the lipidic phases and the incorporation of the membrane protein into such materials can be separated experimentally. Specifically, we have investigated the effect of different initial monoolein-based lipid phase states on the crystallization behavior of the colored photosynthetic reaction center from Rhodobacter sphaeroides. We find that the detergent solubilized photosynthetic reaction center spontaneously inserts into and concentrates in the lipid matrix without any mixing, and that the initial lipid material phase state is irrelevant for productive crystallization. A substantial in-situ enrichment of the membrane protein to concentration levels that are otherwise unobtainable occurs in a thin layer on the surface of the lipidic material. These results have important practical applications and hence we suggest a simplified protocol for membrane protein crystallization within amphiphile rich materials, eliminating any specialized mixing tools to prepare crystallization experiments within lipidic cubic phases. Furthermore, by virtue of sampling a membrane protein concentration gradient within a single crystallization experiment, this crystallization technique is more robust and increases the efficiency of identifying productive crystallization parameters. Finally, we provide a model that explains the incorporation of the membrane protein from solution into the lipid phase via a portal lamellar phase

Self-Assembly in Monoelaidin Aqueous Dispersions: Direct Vesicles to Cubosomes Transition

Background: In the present study, synchrotron small-angle X-ray scattering (SAXS) and Cryo-TEM were used to characterize the temperature-induced structural transitions of monoelaidin (ME) aqueous dispersion in the presence of the polymeric stabilizer F127. We prove that the direct transition from vesicles to cubosomes by heating this dispersion is possible. The obtained results were compared with the fully hydrated bulk ME phase. Methodology/principal findings: Our results indicate the formation of ME dispersion, which is less stable than that based on the congener monoolein (MO). In addition, the temperature-dependence behavior significantly differs from the fully hydrated bulk phase. SAXS findings indicate a direct L(alpha)-V(2) internal transition in the dispersion. While the transition temperature is conserved in the dispersion, the formed cubosomes with internal Im3m symmetry clearly contain more water and this ordered interior is retained over a wider temperature range as compared to its fully hydrated bulk system. At 25 degrees C, Cryo-TEM observations reveal the formation of most likely closely packed onion-like vesicles. Above the lamellar to non-lamellar phase transition at 65 degrees C, flattened cubosomes with an internal nanostructure are observed. However, they have only arbitrary shapes and thus, their morphology is significantly different from that of the well-shaped analogous MO cubosome and hexosome particles. Conclusions/significance: Our study reveals a direct liposomes-cubosomes transition in ME dispersion. The obtained results suggest that the polymeric stabilizer F127 especially plays a significant role in the membrane fusion processes. F127 incorporates in considerable amount into the internal nanostructure and leads to the formation of a highly swollen Im3m phase

Raman spectroscopy studies of liquids confined to porous silica glasses

Raman spectroscopic experiments are presented to investigate the dynamics and structure of liquids confined to porous silica glasses. Using dimethyl sulfoxide (DMSO) as an illustrative example, it is reported that the vibrational correlation time of the SO stretching vibration in DMSO is decreased by confinement and scales with the reciprocal pore radius. This result is interpreted on the basis of a two-state model for surface and bulk molecules. The different contributions to the SO vibrational relaxation are discussed. Furthermore, using the noncoincidence of the isotropic and anisotropic Raman frequencies of the SO vibrational mode, which is a result of an intermolecular vibrational coupling, the surface layer structure of DMSO in pores of silica glass is derived