Influence of sweeteners in the biodistribution of radiopharmaceutical and laboratory tests in rats

This study aimed to evaluate the effect of supplementation of sucralose and fructose on the metabolism of adolescent rats. Eighteen male Wistar rats were divided into 3 groups: control group (GC), fructose group (GF) treated with 50 mg/kg of fructose, and sucralose group (GS) receiving 50 mg/kg of sucralose for 24 days. The weight and feed intake were measured weekly. At the end of the experiment, some biochemical parameters, histopathology of the liver and biodistribution of the radiotracer 99mTc-sodium phytate in liver and blood were analyzed. The GF showed higher body weight only in the first week compared with GS and GC (p<0.05). Histopathology and % ATI/g radiotracer 99mTc-sodium phytate in liver and blood were not different between the groups. The GF showed higher values of aspartate aminotransferase activity, bilirubin, alkaline phosphatase activity and gamma glutamyl transferase activity, compared with the other groups (GC and GS) (p<0.05). Activity of alanine aminotransferase and albumin level of GF were higher than GS (p<0.05). For other parameters, no statistical difference was observed. It was concluded that the use of fructose during the experiment was able to alter hepatic enzymes, but on the other hands, the use of sucralose caused no change.Keywords: Sucralose, fructose, adolescent rats, radiopharmaceutica

Erratum to: The study of cardiovascular risk in adolescents – ERICA: rationale, design and sample characteristics of a national survey examining cardiovascular risk factor profile in Brazilian adolescents

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Papillary Adenocarcinoma of Nasal Sinus in a Dog: Approach Histomorphologic, Immunophenotypic, and Therapeutic

Damasceno, Karine Araújo. “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2018-06-08T12:55:36Z No. of bitstreams: 1 Campos LC Adenocarcinoma....pdf: 498800 bytes, checksum: 12b846e5c3471481ee9a668af22df5de (MD5)Approved for entry into archive by Ana Maria Fiscina Sampaio ([email protected]) on 2018-06-08T13:12:24Z (GMT) No. of bitstreams: 1 Campos LC Adenocarcinoma....pdf: 498800 bytes, checksum: 12b846e5c3471481ee9a668af22df5de (MD5)Made available in DSpace on 2018-06-08T13:12:24Z (GMT). No. of bitstreams: 1 Campos LC Adenocarcinoma....pdf: 498800 bytes, checksum: 12b846e5c3471481ee9a668af22df5de (MD5) Previous issue date: 2013Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Laboratório de Patologia Comparada. Departamento de Biologia Geral. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Laboratório de Patologia Comparada. Departamento de Biologia Geral. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Laboratório de Patologia Comparada. Departamento de Biologia Geral. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Laboratório de Patologia Comparada. Departamento de Biologia Geral. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Laboratório de Patologia Comparada. Departamento de Biologia Geral. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Laboratório de Patologia Comparada. Departamento de Biologia Geral. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Escola de Veterinária. Departamento de Clínica e Cirurgia Veterinária. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Escola de Veterinária. Departamento de Clínica e Cirurgia Veterinária. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Laboratório de Patologia Comparada. Departamento de Biologia Geral. Belo Horizonte, MG, BrasilSinus adenocarcinomas of papillary pattern are neoplasms rarely described in dogs and most cases are characterized by slow growth, local invasion and rare cases of metastasis. Their characteristics are presence of glandular structures that may have papillary pattern, tubulo-papillary, acinar and mixed pattern. Approximately 80% of primary nasal tumors are malignant and adenocarcinomas are the most frequently histology type. This paper reports a case of papillary adenocarcinoma of nasal sinus in the dog, emphasizing the use of histochemical and immunohistochemical techniques to aiding for appropriate therapy. Case: A 14-year-old male Poodle dog, presenting seropurulent nasal discharge, frequent sneezing and an enlargement in the nasal region, was submitted to surgical excision of a tumor. Macroscopically, was observed fragment coated with soft hairy skin and shear blackish color, and some fragments associated with more fi rm tissues like cartilage and bone tissue. Tumor specimens were collected, fi xed in 10% neutral buffered formalin solution and embedded in paraffi n. Afterwards, 4 μm histological sections were obtained and stained with HE. For immunohistochemical analysis a biotin-peroxidase system was used and secondary antibodies were identifi ed using Advance HRP. CKAE1/AE3, CK34βE12, SMA, E-cadherin, Cox-2, Ki-67 and Her-2 expression were evaluated. For CK AE1AE3 antibody a qualitative method was used and neoplasia were classifi ed as negative (-) or positive (+) and for CK34βE12 was used semiquantitative evaluation and neoplasms were classifi ed as negative (-), positive with focal staining (+) and positive with diffuse staining (++). The graduation for HER-2 expression was (+) for 4 (++++), were 4 (++++) was the most intensity membrane staining. For Cox-2 the system used was semiquantitative and the distribution was scored by estimating the percentage of tumor cells staining. Intensity was estimated on a scale from 0 (absent) to 12 (strong). The proliferative index was calculated by counting nuclei positive for Ki-67 (anti-MIB-1) in a total of 1000 neoplastic cells. Microscopically, was observed a proliferation of epithelial cells in papillary arrangement with delicate connective axis of support and areas of stromal invasion, and the tumor received the diagnosis of papillary adenocarcinoma of nasal sinus. Immunohistochemical analysis revealed strong staining for Cox-2 (score 12), CKAE1/AE3 positivity and CK34βE12 (+ + +) and negative for Her-2 (+). Loss of E-cadherin expression was observed and SMA allowed the visualization of areas of basal membrane rupture. The rate of proliferation was evaluated by means of the expression of MIB-1 was high (16%). Chemotherapy was performed with carboplatin and gemcitabine

Physical localization of the retrotransposons Boudicca and Perere 03 in Schistosoma mansoni.

Schistosoma mansoni is 1 of the causative agents of schistosomiasis, an endemic disease in 76 countries of the world. The study of its genome, estimated to be 270 Mb, is very important to understanding schistosome biology, the mechanisms of drug resistance, and immune evasion. Repetitive elements constitute more than 40% of the S. mansoni genome and may play a role in the parasite evolution. The retrotransposons Boudicca, a long terminal repeat (LTR), and Perere 03, a non-LTR, are present in a high number in the S. mansoni genome and were localized with the use of fluorescence in situ hybridization (FISH) and primed in situ labeling (PRINS). Bacterial artificial chromosomes (BAC) clones containing the retrotransposons Boudicca and Perere 03 were selected by bioinformatic analysis and used as probes in FISH. Using metaphase chromosomes from sporocysts and the FISH and PRINS techniques, we were able to map these retrotransposons. Perere 03 was localized in the euchromatic regions of the short arm of chromosome 2 and Boudicca i