Exercise Outcomes in Childhood Obesity-Related Inflammation and Oxidative Status

Childhood obesity is identified as one of the major public health issues to increase the risk for cardiometabolic diseases and related complications in adulthood. The literature has supported inflammation and oxidative stress as the primary underlying mechanisms involved in the pathogenesis of obesity-related diseases. Epidemiological evidence consistently shows the benefits of physical activity in the improvement of obesity-mediated inflammation and oxidative stress status. In this narrative mini-review, the available scientific evidence on the potential effects of exercise in alleviating these susceptibilities in childhood obesity will be assessed.S

Influence of 12-week concurrent training on exosome cargo and its relationship with cardiometabolic health parameters in men with obesity

[EN] Exosome release varies depending on the physiological state of the cell, so they could play a fundamental role in obesity, the biggest pandemic in today's societies. The beneficial effects that physical activity has both on weight and cardiovascular parameters may be mediated by exosomes released in response to exercise. Thus, we aimed (I) to study the influence of a 12-week CT intervention on exosome cargo modifications in men with obesity and (II) to determine whether changes in exosomes after the intervention were related to changes in cardiometabolic health parameters in our cohorts. An experimental, controlled design was performed in twelve (nine with valid data) adult male obese patients (mean values: 41.6 years old, 97.6 kg and 32.4 kg/m2) who were randomly divided into a control group (n = 4) and a training group (n = 5), which completed 36 sessions of CT (concurrent training) for 12 weeks. Before and after the training period, cardiometabolic health parameters were evaluated and blood samples to measure exosomes and proteins were drawn. No changes were observed in the levels of any exosomal markers and proteins; however, associations of changes between CD81 and both fat mass and weight, Flot-1 and VO2max, HSP70 and both CRP and left ventricle diastolic diameter or CD14 and leptin were found (all p ≤ 0.05). Although the current CT was not able to clearly modify the exosome cargo, a certain medium to large clinical effect was manifested considering the nature of this study. Moreover, the associations found between the promoted changes in cardiometabolic parameters and exosome-carried proteins could indicate a relationship to be considered for future treatments in patients with obesity.SICurrent research activities of D.J.-P. are supported by a grant from the Spanish Ministry of Science and Innovation—MINECO (RYC-2014-16938) and the EXERNET Research Network on Exercise and Health in Special Populations (DEP2005-00046/ACTI). Technology Fund—FEDER funds (R&D&I Operating Program) was used by and for the benefit of companies (Technology Fund) INNTERCONECTA (BRISA Contract-University of Cádiz). The results of the study are presented clearly, honestly and without fabrication, falsification or inappropriate data manipulation.Biomedical Research Networking Center on Frailty and Healthy Aging (CIBERFES) and FEDER funds from the European Union (CB16/10/00477)

Effects of exercise on exosome release and cargo in in vivo and ex vivo models: A systematic review

[EN] Exercise-released exosomes have been identified as novel players to mediate cell-to-cell communication in promoting systemic beneficial effects. This review aimed to systematically investigate the effects of exercise on exosome release and cargo, as well as provide an overview of their physiological implications. Among the 436 articles obtained in the database search (WOS, Scopus, and PubMed), 19 articles were included based on eligibility criteria. Results indicate that exercise promotes the release of exosomes without modification of its vesicle size. The literature has primarily shown an exercise-driven increase in exosome markers (Alix, CD63, CD81, and Flot-1), along with other exosome-carried proteins, into circulation. However, exosome isolation, characterization, and phenotyping methodology, as well as timing of sample recovery following exercise can influence the analysis and interpretation of findings. Moreover, a large number of exosome-carried microRNAs (miRNAs), including miR-1, miR-133a, miR-133b, miR-206, and miR-486, in response to exercise are involved in the modulation of proliferation and differentiation of skeletal muscle tissue, although antigen-presenting cells, leukocytes, endothelial cells, and platelets are the main sources of exosome release into the circulation. Collectively, with the physiological implications as evidenced by the ex vivo trials, the release of exercise-promoted exosomes and their cargo could provide the potential therapeutic applications via the role of intercellular communication.SIBrisamar Estébanez was supported by the Ministry of Education of Spain (FPU15/05051). David Jiménez‐Pathe IN MOTU SALUS Research Network on Molecular and Cellular Basis of Physical Exercise for Health and Performance (14/UPB/20)vón was supported by a grant from the Spanish Ministry of Science and Innovation ‐ MINECO (RYC‐2014‐16938). Supported by grants from the EXERNET Research Network on Physical Exercise and Health (09/UPB/20) an

Large-Scale Analysis of User Exposure to Online Advertising on Facebook

Online advertising is the major source of income for a large portion of the Internet Services. There exists a body of literature aiming at optimizing ads engagement, understanding the privacy and ethical implications of online advertising, and so on. However, to the best of our knowledge, no previous work analyzes, at large scale, the exposure of real users to online advertising. This paper performs a comprehensive analysis of the exposure of users to ads and advertisers using a dataset, including more than 7M ads from 140k unique advertisers delivered to more than 5k users, which was collected between October 2016 and May 2018. This paper focuses on Facebook, which is the second largest advertising platform next only to Google in terms of revenue and accounts for more than 2.2B monthly active users. Our analysis reveals that the Facebook users are exposed (in median) to 70 ads per week, which comes from 12 advertisers. Ads represent between 10% and 15% of all the information received in the users' newsfeed. A small increment of 1% in the portion of ads in the newsfeed could roughly represent a revenue increase of 8.17M USD per week for Facebook. Finally, we also reveal that the Facebook users are overprofiled since, in the best case, only 23% of the active interests, Facebook assigns to users for the advertising purpose, are actually related to the ads these users receive.The work of A. A. Galán and R. C. Rumin was supported by the European H2020 Project SMOOTH under Grant 786741. The work of J. G. Cabañas was supported in part by the Ministerio de Economía, Industria y Competitividad, Spain, through the Project TEXEO, under Grant TEC2016-80339-R, and in part by the Ministerio de Educación, Cultura y Deporte, Spain, through FPU, under Grant FPU16/05852. The work of A. Cuevas was supported in part by the Ministerio de Economía, Industria y Competitividad, Spain, and in part by the European Social Fund (EU), through the Ramón Y Cajal, under Grant RyC-2015-17732. The work of M. Calderón was supported by the European H2020 Project TYPES under Grant 653449.Publicad

Mitochondrial function and mitophagy in the elderly: effects of exercise

[EN] Aging is a natural, multifactorial and multiorganic phenomenon wherein there are gradual physiological and pathological changes over time. Aging has been associated with a decrease of autophagy capacity and mitochondrial functions, such as biogenesis, dynamics, and mitophagy. These processes are essential for the maintenance of mitochondrial structural integrity and, therefore, for cell life, since mitochondrial dysfunction leads to an impairment of energy metabolism and increased production of reactive oxygen species, which consequently trigger mechanisms of cellular senescence and apoptotic cell death. Moreover, reduced mitochondrial function can contribute to age-associated disease phenotypes in model organisms and humans. Literature data show beneficial effects of exercise on the impairment of mitochondrial biogenesis and dynamics and on the decrease in the mitophagic capacity associated to aging. Thus, exercise could have effects on the major cell signaling pathways that are involved in the mitochondria quality and quantity control in the elderly. Although it is known that several exercise protocols are able to modify the activity and turnover of mitochondria, further studies are necessary in order to better identify the mechanisms of interaction between mitochondrial functions, aging, and physical activity, as well as to analyze possible factors influencing these processes.S

Biological and psychological monitoring of training status during an entire season in top kayakers

[EN] Aim. The aim of this study was to analyze changes in selected biological and psychological variables in a group of top level kayakers along a 42-week training season. Methods. Eight top junior sprint kayakers (age=16.8±2.1) (5 men and 3 women) with international competitive experience participated in the research. During the 42-wk season the subjects were tested in three occasions: (T1) in the second week of the general training period, (T2) at the beginning of the specific training period, (T3) at the beginning of the competitive training period. Firstly, subjects were asked to complete the Recovery-Stress Questionnaire for Athletes (RESTQ-Sport) and the Profile of Mood States (POMS) questionnaires, and Borg's rate of perceived exertion scale (RPE). Immediately after, blood samples were collected and white blood cells, creatine kinase (CK), C-reactive protein (CRP), myeloperoxidase protein levels (MPO) and glutathione status were determined. ANOVA with repeated measures was used to determine the differences between tests. Results. From the hematological and biochemical measures only total leukocytes changed significantly, increasing at T3 when compared to T1. There were no differences along the entire season in both RESTQ-Sport and POMS scores or indices. Concerning performance, the group improved their maximal strength (+17.4% in bench-press IRM) and their specific-distance time (+9.8%). The main finding of the present study was that training was well-balanced between stress and recovery because while specific performance increased, signs of overtraining were not found. Conclusion. Training monitoring in athletes should be per-formed in a multilevel approach using measurements of performance as well as biological or psychological parameters.S

Resistance training diminishes the expression of exosome CD63 protein without modification of plasma miR-146a-5p and cfDNA in the elderly

[EN] Aging-associated inflammation is characterized by senescent cell-mediated secretion of high levels of inflammatory mediators, such as microRNA (miR)-146a. Moreover, a rise of circulating cell-free DNA (cfDNA) is also related to systemic inflammation and frailty in the elderly. Exosome-mediated cell-to-cell communication is fundamental in cellular senescence and aging. The plasma changes in exercise-promoted miR-146a-5p, cfDNA, and exosome release could be the key to facilitate intercellular communication and systemic adaptations to exercise in aging. Thirty-eight elderly subjects (28 trained and 10 controls) volunteered in an 8-week resistance training protocol. The levels of plasma miR-146a-5p, cfDNA, and exosome markers (CD9, CD14, CD63, CD81, Flotillin [Flot]-1, and VDAC1) were measured prior to and following training. Results showed no changes in plasma miR-146a-5p and cfDNA levels with training. The levels of exosome markers (Flot-1, CD9, and CD81) as well as exosome-carried proteins (CD14 and VDAC1) remained unchanged, whereas an attenuated CD63 response was found in the trained group compared to the controls. These findings might partially support the anti-inflammatory effect of resistance training in the elderly as evidenced by the diminishment of exosome CD63 protein expression, without modification of plasma miR-146a-5p and cfDNA.SIFunding for this project was provided by the Department of Exercise Science and Health Promotion at Florida Atlantic University and supported by grants from the EXERNET Research Network on Physical Exercise and Health (09/UPB/19 and 45/UPB/20) and the IN MOTU SALUS Research Network on Molecular and Cellular Basis of Physical Exercise for Health and Performance (14/UPB/19 and 09/UPB/20). B. Estébanez was supported by a fellowship from the Ministry of Science, Innovation and Universities, Government of Spain (FPU fellowship, reference FPU15/05051; EST18/0025

Aerobic training down-regulates pentraxin 3 and pentraxin 3/toll-like receptor 4 ratio, irrespective of oxidative stress response, in elderly subjects

[EN] Reactive oxygen and nitrogen species-mediated cellular aging has been linked to diseases such as atherothrombosis and cancer. Although pentraxin 3 (PTX3) is associated with aging-related diseases via TLR4-dependent anti-inflammatory effects, its relationship with oxidative stress in aging remains to be elucidated. Exercise is proposed as the key intervention for health maintenance in the elderly. This study aimed to examine the association of PTX3 levels with changes in oxidative stress in both plasma and peripheral blood mononuclear cells (PBMCs), following aerobic training in elderly adults. Nine trained and five controls participated in an eight-week aerobic training protocol. Enzyme-linked immunosorbent assay (ELISA) and Western blot analyses were used to determine PTX3, toll-like receptor 4 (TLR4), and levels of oxidative stress biomarkers [3-nitrotyrosine (3NT), 4-hydroxynonenal (4-HNE), reduced glutathione (GSH), protein carbonyl (PC), reactive oxygen/ nitrogen species (ROS/RNS), trolox equivalent antioxidant capacity (TEAC)] in plasma and/or PBMCs. Results showed a down-regulation of PTX3 expression in PBMCs following aerobic training, along with decreased PTX3/TLR4 ratios. Oxidative stress responses in PBMCs remained unchanged with the exercise protocol. Comparable levels of plasma PTX3 and oxidative stress biomarkers were observed in trained vs. control groups. No correlation was found between PTX3 and any oxidative stress biomarkers following training. These findings demonstrated the down-regulation of PTX3 and PTX3/TLR4 ratio, irrespective of oxidative stress response, in elderly adults following eight weeks of aerobic training.SIFunding for this project was provided by the Department of Exercise Science and Health Promotion at Florida Atlantic University. B. Estébanez was supported by a fellowship from the Ministry of Education of Spain (FPU fellowship, reference FPU15/05051)