Des panoramas de gâchis au nom de la mode: biographie mercantile, géographie du parcellement et gaspillage de vêtements à l’amont du consommateur au Cambodge

This paper advances existing research on both the geographies of fashion and the geographies of waste, utilising their shared interests in commodity biography. Empirically, it documents the use of textile waste from export-oriented garment factories in the peri-urban areas of Phnom Penh, Cambodia as fuel for nearby brick-kilns supplying the city’s booming construction sector. Interviews and documentary photography present the forms of living, labour and harm at both the brick-kilns and the garment dump from which the textile waste is sourced. Substantively, the paper argues for the consideration of preconsumer garment waste to complement the dominant preoccupation with postconsumer waste and reuse. Conceptually, the paper argues for geographical biographies of commodity culture that are attentive to things’ material dynamics of becoming, unbecoming and transformation across spaces, sectors and material forms: that is, to their ‘patch geographies’.Este artículo contribuye con la investigación existente sobre las geografías de la moda y las geografías de los residuos, utilizando sus intereses compartidos en la biografía de las materias primas. Empíricamente, documenta el uso de desechos textiles de fábricas de ropa orientadas a la exportación en las áreas periurbanas de Phnom Penh, Camboya, como combustible para los hornos de ladrillos cercanos que abastecen al sector de la construcción en auge de la ciudad. Las entrevistas y la fotografía documental presentan las formas de vida, trabajo y daños tanto en los hornos de ladrillos como en el basurero de donde provienen los desechos textiles. Sustancialmente, el artículo aboga por la consideración del desperdicio de ropa antes de su consumo para complementar la preocupación dominante con el desperdicio y reutilización post-consumo. Conceptualmente, el artículo aboga por biografías geográficas de la cultura de las materias primas que estén atentas a las dinámicas materiales de convertirse, descomponerse y la transformación a través de los espacios, sectores y formas materiales: es decir, a sus ‘geografías parchadas’ (Tsing, Citation2015).Cette communication joint les courants de recherche existant à la fois dans la géographie de la mode et celle du gaspillage, en utilisant leurs intérêts communs dans la biographie mercantile. Du point de vue empirique, elle documente la manière dont les restes de textiles venant des usines de vêtements pour l’exportation qu’on trouve dans les zones périurbaines de Phnom Penh, au Cambodge, sont utilisés comme combustible pour les usines de briques avoisinantes qui approvisionnent le secteur florissant de la construction de la ville. Des entretiens et des photos-documentaires présentent les formes de vie, de travail et de dangers dans les usines de brique et dans la décharge de vêtements d’où viennent les déchets textiles. Du point de vue substantiel, la communication soutient qu’on devrait prendre en compte ce gaspillage textile à l’amont du consommateur en complément de la préoccupation dominante concernant le gaspillage textile et la réutilisation à l’aval du consommateur. Du point de vue conceptuel, elle se prononce en faveur d’une culture géographique de la biographie mercantile qui se concentrerait sur les dynamiques matérielles des choses qui concernent le devenir, le non-devenir et la transformation à travers les espaces, secteurs et formes matérielles: c’est à dire, à leur « géographie du parcellement » (Tsing, Citation2015)

T Cell Leukemia/Lymphoma 1A is essential for mouse epidermal keratinocytes proliferation promoted by insulin-like growth factor 1

T Cell Leukemia/Lymphoma 1A is expressed during B-cell differentiation and, when overexpressed, acts as an oncogene in mouse (Tcl1a) and human (TCL1A) B-cell chronic lymphocytic leukemia (B-CLL) and T-cell prolymphocytic leukemia (T-PLL). Furthermore, in the murine system Tcl1a is expressed in the ovary, testis and in pre-implantation embryos, where it plays an important role in blastomere proliferation and in embryonic stem cell (ESC) proliferation and self-renewal. We have also observed that Tcl1-/-adult mice exhibit alopecia and deep ulcerations. This finding has led us to investigate the role of TCL1 in mouse skin and hair follicles. We have found that TCL1 is expressed in the proliferative structure (i.e.The secondary hair germ) and in the stem cell niche (i.e.The bulge) of the hair follicle during regeneration phase and it is constitutively expressed in the basal layer of epidermis where it is required for the correct proliferative-differentiation program of the keratinocytes (KCs). Taking advantage of the murine models we have generated, including the Tcl1-/-and the K14-TCL1 transgenic mouse, we have analysed the function of TCL1 in mouse KCs and the molecular pathways involved. We provide evidence that in the epidermal compartment TCL1 has a role in the regulation of KC proliferation, differentiation, and apoptosis. In particular, the colony-forming efficiency (CFE) and the insulin-like growth factor 1 (IGF1)-induced proliferation are dramatically impaired, while apoptosis is increased, in KCs from Tcl1-/-mice when compared to WT. Moreover, the expression of differentiation markers such as cytokeratin 6 (KRT6), filaggrin (FLG) and involucrin (IVL) are profoundly altered in mutant mice (Tcl1-/-). Importantly, by over-expressing TCL1A in basal KCs of the K14-TCL1 transgenic mouse model, we observed a significant rescue of cell proliferation, differentiation and apoptosis of the mutant phenotype. Finally, we found TCL1 to act, at least in part, via increasing phospho-ERK1/2 and decreasing phospho-P38 MAPK. Hence, our data demonstrate that regulated levels of Tcl1a are necessary for the correct proliferation and differentiation of the interfollicular KC

Diversity in Protein Glycosylation among Insect Species

status: publishe

Expression of taste receptors in Solitary Chemosensory Cells of rodent airways

<p>Abstract</p> <p>Background</p> <p>Chemical irritation of airway mucosa elicits a variety of reflex responses such as coughing, apnea, and laryngeal closure. Inhaled irritants can activate either chemosensitive free nerve endings, laryngeal taste buds or solitary chemosensory cells (SCCs). The SCC population lies in the nasal respiratory epithelium, vomeronasal organ, and larynx, as well as deeper in the airway. The objective of this study is to map the distribution of SCCs within the airways and to determine the elements of the chemosensory transduction cascade expressed in these SCCs.</p> <p>Methods</p> <p>We utilized a combination of immunohistochemistry and molecular techniques (rtPCR and in situ hybridization) on rats and transgenic mice where the Tas1R3 or TRPM5 promoter drives expression of green fluorescent protein (GFP).</p> <p>Results</p> <p>Epithelial SCCs specialized for chemoreception are distributed throughout much of the respiratory tree of rodents. These cells express elements of the taste transduction cascade, including Tas1R and Tas2R receptor molecules, α-gustducin, PLCβ2 and TrpM5. The Tas2R bitter taste receptors are present throughout the entire respiratory tract. In contrast, the Tas1R sweet/umami taste receptors are expressed by numerous SCCs in the nasal cavity, but decrease in prevalence in the trachea, and are absent in the lower airways.</p> <p>Conclusions</p> <p>Elements of the taste transduction cascade including taste receptors are expressed by SCCs distributed throughout the airways. In the nasal cavity, SCCs, expressing Tas1R and Tas2R taste receptors, mediate detection of irritants and foreign substances which trigger trigeminally-mediated protective airway reflexes. Lower in the respiratory tract, similar chemosensory cells are not related to the trigeminal nerve but may still trigger local epithelial responses to irritants. In total, SCCs should be considered chemoreceptor cells that help in preventing damage to the respiratory tract caused by inhaled irritants and pathogens.</p

Biopharmaceutical considerations in paediatrics with a view to the evaluation of orally administered drug products – a PEARRL review.

Objectives: In this review, the current biopharmaceutical approaches for evaluation of oral formulation performance in paediatrics are discussed. Key findings: The paediatric gastrointestinal (GI) tract undergoes numerous morphological and physiological changes throughout its development and growth. Some physiological parameters are yet to be investigated, limiting the use of the existing in vitro biopharmaceutical tools to predict the in vivo performance of paediatric formulations. Meals and frequencies of their administration evolve during childhood and affect oral drug absorption. Furthermore, the establishment of a paediatric Biopharmaceutics Classification System (pBCS), based on the adult Biopharmaceutics Classification System (BCS), requires criteria adjustments. The usefulness of computational simulation and modeling for extrapolation of adult data to paediatrics has been confirmed as a tool for predicting drug formulation performance. Despite the great number of successful physiologically based pharmacokinetic models to simulate drug disposition, the simulation of drug absorption from the GI tract is a complicating issue in paediatric populations. Summary: The biopharmaceutics tools for investigation of oral drug absorption in paediatrics need further development, refinement and validation. A combination of in vitro and in silico methods could compensate for the uncertainties accompanying each method on its own

Biopharmaceutics classification system: importance and inclusion in biowaiver guidance

Pharmacological therapy is essential in many diseases treatment and it is important that the medicine policy is intended to offering safe and effective treatment with affordable price to the population. One way to achieve this is through biowaiver, defined as the replacement of in vivo bioequivalence studies by in vitro studies. For biowaiver of new immediate release solid oral dosage forms, data such as intestinal permeability and solubility of the drug are required, as well as the product dissolution. The Biopharmaceutics Classification System (BCS) is a scientific scheme that divides drugs according to their solubility and permeability and has been used by various guides as a criterion for biowaiver. This paper evaluates biowaiver application, addressing the general concepts and parameters used by BCS, making a historical account of its use, the requirements pertaining to the current legislation, the benefits and risks associated with this decision. The results revealed that the use of BCS as a biowaiver criterion greatly expands the therapeutics options, contributing to greater therapy access of the general population with drug efficacy and safety guaranteed associated to low cost

Properties and compartmentalization of digestive carbohydrases and proteases in Scaptotrigona bipunctata (Apidae: Meliponinae) larvae

Aminopeptidase (pH optimum, pHo, 7.5; enzyme relative molecular weights, Mr values: 1, 110 000; 2, 190 000; 3, 300 000), amylase (pHo 5.5, Mr values: 1, 21 000; 2, 68 000); cellobiase (pHo 5.5) and maltase (pHo 5.0, Mr values: 1, 75 000; 2, 110 000; 3, 200 000) are found in the anterior (60-80%) and posterior (20-35%) midgut contents, with minor amounts occurring in midgut cells (2-5%). Trypsin (pHo 7.0, Mr 38 000) occurs mainly in the posterior (62%) rather than in the anterior (37%) midgut contents. Maltase 1 is more active on sucrose than on maltose, the reverse being true for the other maltases. A cysteine-proteinase (pHo 5.6, Mr 79 000) was found in major amounts in the pollen grains ingested by the larvae. The results suggest that, except for a cysteine-proteinase derived from ingested pollen, all digestive enzymes originate in the midgut tissue and are most active in the luminal contents. Evidence is presented supporting the hypothesis that enzymes and nutrients diffusing through the peritrophic membrane are translocated forward by a countercurrent flux. The absence of a midgut differentiation of midgut luminal pH in S bipunctata larvae is though to be derived from putative Hymenopteran ancestors

The Aedes aegypti larval transcriptome: a comparative perspective with emphasis on trypsins and the domain structure of peritrophins

The genome sequence of Aedes aegypti was recently reported. A significant amount of Expressed Sequence Tags (ESTs) were sequenced to aid in the gene prediction process. In the present work we describe an integrated analysis of the genomic and EST data, focusing on genes with preferential expression in larvae (LG), adults (AG) and in both stages (SG). A total of 913 genes (5.4% of the transcript complement) are LG, including ion transporters and cuticle proteins that are important for ion homeostasis and defense. From a starting set of 245 genes encoding the trypsin domain, we identified 66 putative LG, AG, and SG trypsins by manual curation. Phylogenetic analyses showed that AG trypsins are divergent from their larval counterparts (LG), grouping with blood-induced trypsins from Anopheles gambiae and Simulium vittatum. These results support the hypothesis that blood-feeding arose only once, in the ancestral Culicomorpha. Peritrophins are proteins that interlock chitin fibrils to form the peritrophic membrane (PM) that compartmentalizes the food in the midgut. These proteins are recognized by having chitin-binding domains with 6 conserved Cys and may also present mucin-like domains (regions expected to be highly O-glycosylated). PM may be formed by a ring of cells (type 2, seen in Ae. aegypti larvae and Drosophila melanogaster) or by most midgut cells (type 1, found in Ae. aegypti adult and Tribolium castaneum). LG and D. melanogaster peritrophins have more complex domain structures than AG and T. castaneum peritrophins. Furthermore, mucin-like domains of peritrophins from T. castaneum (feeding on rough food) are lengthier than those of adult Ae. aegypti (blood-feeding). This suggests, for the first time, that type 1 and type 2 PM may have variable molecular architectures determined by different peritrophins and/or ancillary proteins, which may be partly modulated by diet.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)FAPESP Fundacao de Amparo a Pesquisa do Estado de Sao PauloFAPESPFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)CNPq Conselho Nacional de Desenvolvimento Cientifico e TecnologicoConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)CNP