Framing the Genetic Engineering Debate: An Examination of Frames and Sources in Local Newspaper Reporting

The study contributes to understanding of how a scientific controversy – genetic engineering – is treated in news stories in local newspapers. The findings provide quantitative evidence that local newspaper coverage of genetic engineering issues is framed in diverse and complex ways. Additionally, the analyses reveal that oppositional viewpoints exist in some local newspapers, perhaps more so than in national news. In contrast to studies of biotechnology news content in the national, elite press, this study suggests that a range of voices and interpretations about biotechnology do in fact exist in news media coverage of biotechnology in the United States, at least in some local newspapers. The research specifically focuses on news media framing of genetic engineering and how stakeholders in the debate influence those frames. A computer-assisted content analysis was conducted on local newspaper coverage related to agricultural biotechnology. Semi-structured interviews with dominant stakeholders were conducted to augment quantitative evidence of news frames. Methodologically, the dissertation introduces and elaborates the use of computer-assisted content analysis to determine frames related to biotechnology. The WordStat computer program was employed to systematically identify and analyze frames and frame changes over time. Moreover, unlike previous framing studies that have used cluster analysis, this study details the usefulness of factor analysis in statistically validating frames. This study identifies and compares news frames in local newspapers in Northern California and in the St. Louis (Missouri) Post-Dispatch. News articles that contained keywords pertaining to genetically modified organisms (crops and food) from January 1992 to December 2004 were obtained for the analysis from the Lexis-Nexis Academic database. A total of 1,134 news articles from the St. Louis Post-Dispatch was collected; 860 of these news articles were retained for analysis. A total of 508 news articles from four Northern California newspapers was collected; 296 of these news articles were analyzed. Additionally, quantitative analyses of dominant stakeholders mentioned in both the Missouri and Northern California news articles were conducted. To supplement the quantitative findings, interviews with nine of the dominant stakeholders, or news sources, identified in the news articles investigated the stakeholders’ involvement in shaping news media coverage of agri-food biotechnology issues. Substantively, this study offers some understanding of the place of dissenting voices in localized debates on genetic engineering. The discovery that local news frames stories on biotechnology in greater complexity raises larger questions about the importance and value of local and community news. Thus, the study addresses the vital need for investigating news content in local news media

A Phase I/II Study of a 72-h Continuous Infusion of Etoposide in Advanced Soft Tissue Sarcoma

Purpose. The study was performed to assess the antitumour activity and toxicity of a 72-h continuous infusion of single-agent etoposide as second-line treatment for patients with locally advanced or metastatic soft tissue sarcoma (STS), following reports of substantial activity using this schedule of etoposide administration as first-line treatment in combination with ifosfamide

Identifying prognostic indicators for cognitive stimulation therapy for dementia: protocol for a systematic review and individual participant data meta-analysis

BACKGROUND: Cognitive stimulation therapy (CST) is the only non-pharmacological, treatment for dementia recommended by the UK National Institute for Health and Care Excellence, following multiple international trials demonstrating beneficial cognitive outcomes in people with mild-to-moderate dementia. However, there is limited understanding of whether treatment prognosis is influenced by sociodemographic and clinical variables (such as dementia subtype and gender), information which could inform clinical decision-making. AIM: We describe the protocol for a systematic review and individual patient data meta-analysis assessing the prognostic factors related to CST. In publishing this protocol, we hope to increase the transparency of our work, and keep healthcare professionals aware of the latest evidence for effective CST. METHOD: A systematic review will be conducted with searches of the bibliographic databases Medline, EMBASE and PsycINFO, from inception to 7 February 2023. Studies will be included if they are clinical trials of CST, use the Alzheimer's Disease Assessment Scale - Cognitive Subscale (gold-standard measure of cognition in dementia in clinical trials) and include participants with mild-to-moderate dementia. Following harmonisation of the data-set, mixed-effect models will be constructed to explore the relationship between the prognostic indicators and change scores post-treatment. CONCLUSIONS: This is the first individual patient data meta-analyses on CST, and has the potential to significantly optimise patient care. Previous analyses suggest people with advanced dementia could benefit more from CST treatment. Given that CST is currently used post-diagnosis in people with mild-to-moderate dementia, the implications of confirming this finding, among identifying other prognostic indicators, are profound

Validation of a Model for Prediction of Percent Intramuscular Fat on Live Feedlot Cattle

A prediction model from a previous study was utilized to evaluate the degree of fit when this model is applied to an independent data set. The degree of fit was evaluated using means, regression analysis, correlation coefficient, distribution of residuals, and mean square error of prediction (MSEP). The model provided a reasonably accurate prediction of intramuscular fat with a mean bias of 0.13%. For 47.1% of the steers, percent intramuscular fat was predicted within ± 0.5%, and for 77.6% of the steers, prediction of percent intramuscular fat was made within ± 1%. Pearson product moment correlation between predicted and actual percent intramuscular fat was 0.74 (p \u3c .01), and the square root of MSEP indicated a prediction error of 0.9%

Qualitative study of the acceptability and feasibility of acceptance and commitment therapy for adolescents with chronic fatigue syndrome

BACKGROUND: Paediatric chronic fatigue syndrome/myalgic encephalomyelitis (CFS/ME) is disabling and relatively common. Although evidenced-based treatments are available, at least 15% of children remain symptomatic after one year of treatment. Acceptance and commitment therapy (ACT) is an alternative therapy option; however, little is known about whether it is an acceptable treatment approach. Our aim was to find out if adolescents who remain symptomatic with CFS/ME after 12 months of treatment would find ACT acceptable, to inform a randomised controlled trial (RCT) of ACT. METHODS: We recruited adolescents (diagnosed with CFS/ME; not recovered after one year of treatment; aged 11–17 years), their parent/carer and healthcare professionals (HCPs) from one specialist UK paediatric CFS/ME service. We conducted semi-structured interviews to explore barriers to recovery; views on current treatments; acceptability of ACT; and feasibility of an effectiveness RCT. Thematic analysis was used to identify patterns in data. RESULTS: Twelve adolescents, eleven parents and seven HCPs were interviewed. All participants thought ACT was acceptable. Participants identified reasons why ACT might be efficacious: pragmatism, acceptance and compassion are valued in chronic illness; values-focussed treatment provides motivation and direction; psychological and physical needs are addressed; normalising difficulties is a useful life-skill. Some adolescents preferred ACT to cognitive behavioural therapy as it encouraged accepting (rather than challenging) thoughts. Most adolescents would consent to an RCT of ACT but a barrier to recruitment was reluctance to randomisation. All HCPs deemed ACT feasible to deliver. Some were concerned patients might confuse ‘acceptance’ with ‘giving up’ and called for clear explanations. All participants thought the timing of ACT should be individualised. CONCLUSIONS: All adolescents with CFS/ME, parents and HCPs thought ACT was acceptable, and most adolescents were willing to try ACT. An RCT needs to solve issues around randomisation and timing of the intervention

Evaluation of Ultrasound Measurement of Fat Thickness and Ribeye Area, II. Repeatability of Measurements.

Data from two feeding trials were used to evaluate repeatability of ultrasound measurements of fat thickness and ribeye area. In each trial, steers were scanned three or four times by one technician. Two beef improvement federation (BIF)-certified technicians with different levels of experience interpreted images from the last scan. Each technician interpreted the image of an individual steer twice on two different days. Repeatability was evaluated as an intra-class correlation. Additional statistics used to evaluate repeatability were the slope and intercepts from a regression analysis, RMSE, and ESD. Ultrasound measurements of fat thickness and ribeye area were repeatable both within and across technicians. The only exception was the across-technician measurements of ribeye area, where an apparent difference in variances of measurements was observed

Evaluation of Ultrasound Measurements of Fat Thickness and Ribeye Area, I. Assessment of Technician Effect on Accuracy

Data from two feeding trials were used to estimate accuracy of ultrasound measurements of fat thickness and ribeye area. In each trial, steers were scanned three or four times by one technician. Two beef improvement federation (BIF)-certified technicians with different levels of experience interpreted images from the last scan taken just before slaughter. Each technician interpreted the image of an individual steer twice on two different days. Accuracy of interpretation was evaluated using simple statistical measures, including means, standard deviations, regression and correlation coefficients, RMSE, and ESD. The overall technician biases for ultrasound measurements of fat thickness and ribeye area were -0.17 cm and 0.63 cm2, respectively. Mean bias by technician indicated a similar direction and amount of bias (-0.14 vs -0.20 cm). However, bias in the measurement of ribeye area by the two technicians took an opposite direction ( -1.28 vs 2.54 cm2). In all cases, technician bias was within the acceptable range for BIF certification. Pearson product moment correlations between carcass and ultrasound measurements of fat thickness and ribeye area were 0.70 and 0.40, respectively. In general, fat thickness for 52% of the steers was measured within ±0.254 cm and for 85.2 % of the steers, fat thickness was measured within ±0.508 cm. For ribeye area, ±51.2 % and ±71.4 % of the steers had measurements within ±6.65 cm2 and ±12.99 cm2, respectively