Clouds

Non-fiction by Jane Co

THE ACOUSTIC EMISSIONS PRODUCED BY ESCHERICHIA COLI DURING THE GROWTH CYCLE

The objective of this study was to determine if acoustic emissions (AE) generated by three strains of Escherichia Coli (5024-parent strain, 8279-mutant strain and 8279-random/unrelated strain) could be used to differentiate each strain during their growth cycle. An acoustic sensor with an operating range of 35 kHz-100 kHz was inserted into the growth vessel and attached to a selected channel to capture AE data. The growth vessel was loaded with 60 ml of tryptic soy broth (TSB) (0.25% fructose) media with alginate (1.1%) or without alginate and inoculated with 1% (108 CFU/ml) of an E. coli strain. The growth vessel was placed in a monitoring chamber and incubated at 32°C for 8-9 h. The AE’s generated by each strain were collected throughout the growth cycle. All strains grown in media with and without alginate generated AE’s within 5 min post inoculation. Strains grown in media without alginate generated stronger (P \u3c 0.0001) absolute energy (ABSE) and higher peak frequencies (PFRQ’s), than in media with alginate. The AE’s generated by strains 5024 and 8237 were stronger and easily distinguished from those generated by strain 8279. Strain 8237 generated 12% stronger ABSE from the 3rd to 8th h and 51% stronger PFRQ intensities than strain 5024 during 0-8 h. However, strain 5024 generated 15% stronger ABSE and 31% higher PFRQ’s during the final hour of growth. Strain 5024 generated the highest PFRQ’s from 5-50 kHz, while strain 8237 generated higher frequencies from 100-500 kHz. Fourteen distinguishable differences (P\u3c 0.05) in generated PFRQ’s, between strains 5024 and 8237, were also observed in every 5 kHz increments from 100-500 kHz. Of these differences, strain 8237 generated higher frequencies within eight of the kHz ranges, while strain 5024 generated higher frequencies within six other kHz ranges. These data suggests that all bacteria may generate different AE’s, thus producing a unique “fingerprint” of sound that will allow for its identification

Action Research: A Clinical Case in Remedial Reading

In setting up the problem, it was necessary to propose a plan of action, try the plan or plans, gain evidence, and write the results. The report would be anecdotal, descriptive, and so organized as to give, as much as possible, a lucid and over-all picture of what had taken place. To maintain anonymity, the child would be given another name and the terms examiner, researcher, and other similar general references would be used. The problem then became the factual account of an ongoing project in remedial instruction under competent guidance in the hope of arriving at assumptions and recommendations that would prove of value to others interested in a similar activity

Analysis and critique of ‘Transforming children and young people’s mental health provision: A green paper’: Some implications for refugee children and young people

Adopting a children’s rights perspective, a critique and analysis underpinned by documentary research methodology was undertaken in order to assess the extent to which the government’s Green Paper (Department of Health and Social Care and Department of Education, 2017. Transforming children and young people’s mental health provision: a green paper. Available at: https://www.gov.uk/government/consultations/transforming-children-and-young-peoples-mental-health-provision-a-green-paper (accessed 7 December 2017)) addresses the mental health and well-being needs of refugee children and young people in England and Wales, identifying strengths, limitations and challenges for future policy and practice. Findings suggest that there is much of potential benefit to refugee children and young people’s future mental health and well-being. However, a paradigm shift, explicit in implications, scale and time frame, will be required, if the Green Paper is to achieve those changes in attitudes, practice and service delivery which it anticipates. We argue that this Green Paper’s overarching challenge is that it is premised on Western-centric models in its understanding of the experiences of refugee children and young people, and management of trauma and mental health. It fails to recognize the meanings and significance of culture, and of diversity and difference, and the need to invest in all communities in facilitating engagement and support for children and young people’s mental health issues

Prospects and challenges in the development of universal influenza vaccines

Current influenza vaccines offer suboptimal protection and depend on annual reformulation and yearly administration. Vaccine technology has rapidly advanced during the last decade, facilitating development of next-generation influenza vaccines that can target a broader range of influenza viruses. The development and licensure of a universal influenza vaccine could provide a game changing option for the control of influenza by protecting against all influenza A and B viruses. Here we review important findings and considerations regarding the development of universal influenza vaccines and what we can learn from this moving forward with a SARS-CoV-2 vaccine design.publishedVersio

Embedding the provision of information and consultation in the workplace: a longitudinal analysis of employee outcomes in 1998 and 2004

Based on an analysis of the Workplace Employment Relations Survey, this report finds strong positive links between the ‘breadth’ and ‘depth’ of some information and consultation practices and employee commitment. Employee ratings of the helpfulness of some consultation and communication methods are positively linked to job satisfaction and commitment. Employee ratings of managers’ effectiveness in consulting employees and employees’ satisfaction with their involvement in decision-making are also positively linked with job satisfaction and commitment, suggesting that the way in which information and consultation methods are implemented is just as important as the type of practices used.This report was commissioned by DTI under the Workplace Employment Relations Survey (WERS) 2004 Grants Fund. The Fund is a Department of Trade and Industry initiative to develop the evidence base in areas of policy interest, raise awareness of this survey and encourage advanced data analysis based on the WERS 2004 datasets

Preservice Elementary Teachers’ Beliefs about the Role of Definition in the Learning of Mathematics

Answering a call to emphasize the act of defining over the learning of definitions, we have shifted the content of a geometry course for preservice elementary teachers (PSETs) away from comparing and applying pre-written classification structures to classroom episodes centered on authoring definitions for special quadrilaterals. PSETs complete activities using geometry software, and collaboratively create definitions for specific quadrilaterals. In order to fully understand the potential of this curricular shift, we asked preservice elementary mathematics teachers’ to share their perceptions of the process of writing mathematical definitions. Data from participant reflections were analyzed for themes related to mathematical definition and the act of defining. The framework that resulted from iterative discussions by the researchers examined beliefs about the nature of definition and mathematical empathy (Araki, 2015). Findings also suggest that beliefs about authority and positioning students as authors of mathematics are associated with mathematical empathy. Experiences related to the process of defining enabled PSETs to see far greater subjectivity in the discipline of mathematics and to consider, perhaps for the first time, that they, too, were both able and deserving of becoming authors of mathematical ideas