Regulation of intracellular cyclic GMP concentration by light and calcium in electropermeabilized rod photoreceptors.

Abstract This study examines the regulation of cGMP by illumination and by calcium during signal transduction in vertebrate retinal photoreceptor cells. We employed an electropermeabilized rod outer segment (EP-ROS) preparation which permits perfusion of low molecular weight compounds into the cytosol while retaining many of the features of physiologically competent, intact rod outer segments (ROS). When nucleotide-depleted EP-ROS were incubated with MgGTP, time- and dose-dependent increases in intracellular cGMP levels were observed. The steady state cGMP concentration in EP-ROS (0.007 mol cGMP per mol rhodopsin) approached the cGMP concentration in intact ROS. Flash illumination of EP-ROS in a 250-nM free calcium medium resulted in a transient decrease in cGMP levels; this occurred in the absence of changes in calcium concentration. The kinetics of the cGMP response to flash illumination of EP-ROS were similar to that of intact ROS. To further examine the effects of calcium on cGMP metabolism, dark-adapted EP-ROS were incubated with MgGTP containing various concentrations of calcium. We observed a twofold increase in cGMP steady state levels as the free calcium was lowered from 1 μM to 20 nM; this increase was comparable to the behavior of intact ROS. Measurements of guanylate cyclase activity in EP-ROS showed a 3.5-fold increase in activity over this range of calcium concentrations, indicating a retention of calcium regulation of guanylate cyclase in EP-ROS preparations. Flash illumination of EP-ROS in either a 50- or 250-nM free calcium medium revealed a slowing of the recovery time course at the lower calcium concentration. This observation conflicts with any hypothesis whereby a reduction in free calcium concentration hastens the recovery of cytoplasmic cGMP levels, either by stimulating guanylate cyclase activity or by inhibiting phosphodiesterase activity. We conclude that changes in the intracellular calcium concentration during visual transduction may have more complex effects on the recovery of the photoresponse than can be accounted for solely by guanylate cyclase activation

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The Formation of Giant Elliptical Galaxies and Their Globular Cluster Systems

The bimodal globular cluster (GC) metallicity distributions of many giant elliptical galaxies are often cited as evidence for the formation of such galaxies through mergers involving gas-rich spirals. In such models, the metal- rich GCs are assumed to have formed during the merger process. We explore an alternative possibility: that these metal-rich clusters represent the galaxy's intrinsic GC population and that the metal-poor component of the observed GC metallicity distribution arises from the capture of GCs from other galaxies, either through mergers or through tidal stripping. Starting with plausible assumptions for the initial galaxy luminosity function and for the dependence of GC metallicity on parent galaxy luminosity, we show that the growth of a pre-existing seed galaxy through mergers and tidal stripping is accompanied by the capture of metal-poor GCs whose properties are similar to those which are observed to surround giant ellipticals. We describe a method of using the observed number of metal-poor and metal-rich GCs to infer the merger histories of individual elliptical galaxies, and use this technique to derive limits on the number of galaxies and total luminosity accreted to date by M49. We argue that although GC specific frequency is conserved in galaxy mergers, the same may not be true of tidal stripping by the mean field of the host galaxy cluster. Comparisons of model GC metallicity distributions and specific frequencies to those observed for the well-studied galaxies M49 and M87 show that it is possible to explain their bimodal GC metallicity distributions and discordant specific frequencies without resorting to the formation of new GCs in mergers or by invoking multiple bursts of GC formation.Comment: 39 pages AAS Latex and 10 postscript figures. Also available at http://astro.caltech.edu/~pc. Accepted for publication in the Astrophysical Journa

Alien Registration- Cote, Andre J. (Sanford, York County)

https://digitalmaine.com/alien_docs/3137/thumbnail.jp

Alien Registration- Cote, Lawrence J. (Anson, Somerset County)

https://digitalmaine.com/alien_docs/9138/thumbnail.jp