3D HDO-CLEM: cellular compartment analysis by correlative light-electron microscopy on cryosections.

Fundamental to obtaining a depth-understanding of the function and structure of cells is the ability to study and correlate their molecular topography with the ultrastructural morphology, for example, to visualize the position of a given protein relative to a given cell compartment and its morphology. Standard fluorescence light microscopy (FLM) relies on simple sample preparations, and localizes proteins in living or fixed cells with a resolution in the range of few hundred nanometers, allowing large field of view. However, FLM is unable to visualize the unlabeled cellular context. On the other hand, electron microscopy (EM) techniques reveal protein topology with the resolution in a range of a few tens of nanometer, retains the cellular context, but can only be applied on a limited field of view. Therefore, both approaches present shortcomings, in terms of field of view, statistical output, resolution, sample preparation, and context analysis, that can likely complement each other. To bridge the gap between FLM imaging and EM, several laboratories have developed methods for correlative light-electron microscopy (CLEM). In a nutshell, CLEM enables one to investigate the same exact region of interest utilizing the two microscope platforms, and thereby virtually combine their capabilities. We describe a protocol based on immunolabeling of Tokuyasu cryosections that allows correlation of LM and EM images with excellent preservation of cellular ultrastructure. We will refer to this method as high-data-output CLEM (HDO-CLEM). The major benefits of HDO-CLEM are the possibility to (1) correlate several hundreds of events at the same time, (2) perform three-dimensional (3D) correlation, (3) immunolabel both endogenous and recombinantly tagged proteins at the same time, and (4) combine the high data analysis capability of FLM with the high precision of transmission EM in a CLEM hybrid morphometric analysis. We have identified and optimized critical steps in sample preparation, defined routines for sample analysis and retracing of regions of interest, developed software for semi/fully automatic 3D FLM reconstruction and set the basis for a hybrid light/EM morphometry approach

Booze and butts: A content analysis of the presence of alcohol in tobacco industry lifestyle magazines

published_or_final_versio

Deriving a multivariate CO-to-H2_2 conversion function using the [CII]/CO(1-0) ratio and its application to molecular gas scaling relations

We present Herschel PACS observations of the [CII] 158 micron emission line in a sample of 24 intermediate mass (9<logM$_\ast$/M$_\odot$<10) and low metallicity (0.4< Z/Z$_\odot$<1.0) galaxies from the xCOLD GASS survey. Combining them with IRAM CO(1-0) measurements, we establish scaling relations between integrated and molecular region [CII]/CO(1-0) luminosity ratios as a function of integrated galaxy properties. A Bayesian analysis reveals that only two parameters, metallicity and offset from the star formation main sequence, $\Delta$MS, are needed to quantify variations in the luminosity ratio; metallicity describes the total dust content available to shield CO from UV radiation, while $\Delta$MS describes the strength of this radiation field. We connect the [CII]/CO luminosity ratio to the CO-to-H$_2$ conversion factor and find a multivariate conversion function $\alpha_{CO}$, which can be used up to z~2.5. This function depends primarily on metallicity, with a second order dependence on $\Delta$MS. We apply this to the full xCOLD GASS and PHIBSS1 surveys and investigate molecular gas scaling relations. We find a flattening of the relation between gas mass fraction and stellar mass at logM$_\ast$/M$_\odot$<10. While the molecular gas depletion time varies with sSFR, it is mostly independent of mass, indicating that the low L$_{CO}$/SFR ratios long observed in low mass galaxies are entirely due to photodissociation of CO, and not to an enhanced star formation efficiency.Comment: Submitted to MNRAS, this version after referee comments. 21 page

The selective effect of environment on the atomic and molecular gas-to-dust ratio of nearby galaxies in the Herschel Reference Survey

We combine dust, atomic (HI) and molecular (H$_{2}$) hydrogen mass measurements for 176 galaxies in the Herschel Reference Survey to investigate the effect of environment on the gas-to-dust mass ($M_{\rm gas}/M_{\rm dust}$) ratio of nearby galaxies. We find that, at fixed stellar mass, the average $M_{\rm gas}/M_{\rm dust}$ ratio varies by no more than a factor of $\sim$2 when moving from field to cluster galaxies, with Virgo galaxies being slightly more dust rich (per unit of gas) than isolated systems. Remarkably, once the molecular and atomic hydrogen phases are investigated separately, we find that \hi-deficient galaxies have at the same time lower $M_{\rm HI}/M_{\rm dust}$ ratio but higher $M_{\rm H_{2}}/M_{\rm dust}$ ratio than \hi-normal systems. In other words, they are poorer in atomic but richer in molecular hydrogen if normalized to their dust content. By comparing our findings with the predictions of theoretical models, we show that the opposite behavior observed in the $M_{\rm HI}/M_{\rm dust}$ and $M_{\rm H_{2}}/M_{\rm dust}$ ratios is fully consistent with outside-in stripping of the interstellar medium (ISM), and is simply a consequence of the different distribution of dust, \hi\ and H$_{2}$ across the disk. Our results demonstrate that the small environmental variations in the total $M_{\rm gas}/M_{\rm dust}$ ratio, as well as in the gas-phase metallicity, do not automatically imply that environmental mechanisms are not able to affect the dust and metal content of the ISM in galaxies.Comment: 11 pages, 6 figures, 2 tables. Accepted for publication in MNRA

Witnessing galaxy preprocessing in the local Universe: the case of a star-bursting group falling into Abell 1367

We present a multiwavelength analysis of a compact group of galaxies infalling at high speed into the dynamically young cluster Abell 1367. Peculiar morphologies and unusually high Halpha emission are associated with two giant galaxies and at least ten dwarfs/extragalactic HII regions, making this group the region with the highest density of star formation activity ever observed in the local clusters. Moreover Halpha imaging observations reveal extraordinary complex trails of ionized gas behind the galaxies, with projected lengths exceeding 150 kpc. These unique cometary trails mark the gaseous trajectory of galaxies, witnessing their dive into the hot cluster intergalactic medium. Under the combined action of tidal forces among group members and the ram-pressure by the cluster ambient medium, the group galaxies were fragmented and the ionized gas was blown out. The properties of this group suggest that environmental effects within infalling groups may have represented a preprocessing step of the galaxy evolution during the high redshift cluster assembly phase.Comment: 23 pages, 13 figures, 5 table. Accepted for publication in Astronomy & Astrophysics main journal. Version with high-resolution images available at http://goldmine.mib.infn.it/papers/preprocessing.htm

The Arecibo Galaxy Environment Survey: II. A HI view of the Abell cluster 1367 and its outskirts

We present 21 cm HI line observations of 5x1 square degrees centered on the local Abell cluster 1367 obtained as part of the Arecibo Galaxy Environment Survey. One hundred sources are detected (79 new HI measurements and 50 new redshifts), more than half belonging to the cluster core and its infalling region. Combining the HI data with SDSS optical imaging we show that our HI selected sample follows scaling relations similar to the ones usually observed in optically selected samples. Interestingly all galaxies in our sample appear to have nearly the same baryon fraction independently of their size, surface brightness and luminosity. The most striking difference between HI and optically selected samples resides in their large scale distribution: whereas optical and X-ray observations trace the cluster core very well, in HI there is almost no evidence of the presence of the cluster. Some implications on the determination of the cluster luminosity function and HI distribution for samples selected at different wavelength are also discussed.Comment: 22 pages, 15 figures, 4 tables. Accepted for publication on MNRAS Main Journal. High resolution version of this paper can be downloaded at http://www.astro.cf.ac.uk/pub/Luca.Cortese/papers/ages_a1367.pdf . Datacubes and catalogs can be downloaded at http://www.naic.edu/~ages/public_data.htm