ABO Blood Groups in Systemic Sclerosis: Distribution and Association with This Disease’s Characteristics

Systemic sclerosis (SSc) is an autoimmune disease associated with endothelial activation and fibrosis. Non-O blood group patients carry an increased risk of thrombosis, fibrosis and autoimmune diseases. The aim of our work was to evaluate the distribution of ABO groups in SSc patients and their association with the disease’s characteristics. ABO groups were determined in 504 SSc patients (with 131 completed by a genotypic analysis). The distribution of ABO groups and their diplotypes in SSc patients was comparable to that of the general population, except for haplotypes O1 and B (65.6% vs. 61.6% and 8.8% vs. 5.8% in SSc patients vs. the general population, respectively, p = 0.01). The frequency of interstitial lung disease, pulmonary hypertension, calcinosis, digital ulcers, digestive diseases and venous thrombosis, and the Medsger score, were higher in non-O than in O-SSc patients, although they did not display statistical significance. Patients in the non-O group had higher levels of inflammation and endothelial activation biomarkers. In conclusion, the ABO blood group distribution of SSc patients did not differ significantly from that of the general population, but non-O blood groups were associated with inflammation and endothelial activation, and with a non-significant higher frequency of pulmonary and vascular complications in SSc

Improvement of Autobiographical Memory in Schizophrenia Patients Following a Cognitive Intervention: A Preliminary Study.

Background: Schizophrenia is associated with a reduction in accessing specific autobiographical information. This is consistent with the abnormal development of the personal identity characterizing this mental disorder. The present study evaluates the effect of a cognitive intervention on autobiographical memory and on the capacity to project oneself in the future in people suffering from schizophrenia. Sampling and Method: The intervention consisted of group sessions during which participants were trained to recollect specific events reported in their diary. Further, exercises to stimulate their thoughts on their personal identity were proposed. An AM test was administrated before and after the intervention, as well as at three months follow-up. Further, neuropsychological and affective assessments were conducted before and after treatments. Patients’ performances were compared to those from the control group Results: The capacities to generate specific events are improved by the cognitive intervention, and that the benefits are preserved 3 months later. However, no neuropsychological or affective benefit was found. Conclusion: Despite positive results on specific memory, the significant benefits have to be extended to other clinical variables such as symptom reduction, neuropsychological and social functioning. Nevertheless, the results revealed that CRT on AM could be a useful additional intervention for schizophrenia patients

Aeolian bedforms formed by ice sublimation and vapor condensation on Louth crater ice, Mars.

International audience<p><strong>Introduction:</strong> Louth Crater is a 36 km diameter located at 70 °N, 103.2 °E (Fig. 1) less than 1000 km from the Martian North Polar Cap.  At the center of Louth crater a perennial water ice cap ~10 km in diameter, ~250 m in width (Fig. 1) that undergoes phase changes (condensation / sublimation cycles) during the Martian year [1 - 4]. Some periodic structure at the surface of its perennial water ice cap have been observed [2]. The presence of smaller ice undulations superimposed on this periodic structure, comparable to the sublimation waves on the Martian North Polar Cap [5] could be formed during sublimation and condensation period of the water ice.  The characterization of this ice waves could make it possible to specify environmental conditions favorable to their formation. The first part of the study consists of identifying these ice waves using orbital topography and imaging data, and then associating these results with data from the Martian Database [6-7] and with the scaling laws inherent to the formation of sublimation and condensation waves.<strong> </strong></p> <p><img src="" alt="" /></p> <p><strong>Fig. 1:</strong> Louth crater in summer. CTX product J02_045439_2504_XN_70N256W Ls = 133.2°</p> <p><strong>Geomorphological analysis:</strong></p> <p><em>Methods:</em> Digital Elevation Model (DEM) at ~100 m/pixel and MOLA elevation data at ~128 m/pixel have been coupled with imagery data from HRSC ~10 m/pixel, CTX at ~6 m/pixel and HiRISE images for more precise areas for up to 25 cm/pixel. Data were georeferenced in ESRI’s ArcMap GIS software to produce geomorphological map and analyzed to evaluate ice waves shape and spatial organization.</p> <p><em>Observations:</em> Louth presents two units (Fig. 2). (a) Lower unit with dark stucco texture and stratifications (Fig. 3a). (b) Fresh ice overlies this older, stratified structure (Fig 3.a). This fresh ice is distributed in a non-uniform manner as shown by the kilometrics waves of about 560 m wavelength on which are superimposed decametrics waves of about 55 m wavelength which are both perpendicular to the prevailing wind (Fig 3.b). The crests of both wave populations show similar NW-SE. The main wind direction was assessed from barkhane field on the sand mound.</p> <p><img src="" alt="" /></p> <p><strong>Fig. 2:</strong> Louth’s geomorphological map in summer. CTX product J02_045439_2504_XN_70N256W Ls = 133.2°.</p> <p> </p> <p><img src="" alt="" /></p> <p> </p> <p><strong>Fig. 3:</strong>  Boxes from Fig. 2. (a) East side of the Louth crater ice cap. Green lines represents stratifications, HiRISE product EPS_045439_2505, Ls = 133.2°. (b) decametric waves on the ice waves. Black dashes represent leeside foot of each kilometric waves. Blue lines emphasize decametric waves, same HiRISE product from (a).</p> <p><strong>Transport hypothesis:</strong> Preliminary experiments studied the threshold velocity necessary to initiate a transport of ice grains in Martian Simulation Wind Tunnel [8]. These experiments were carried out for grains from a few hundred micrometers to 2 mm in diameter under atmospheric pressures of 40 to 1000 mbar. To transport ice particles under near-Martian condition is hard because the threshold velocity is ten times higher than Earth. The wind speeds estimated from the Martian Database [6-7] in Louth crater are much lower than those needed to transport ice grains at Martian pressure. We support the hypothesis of diffusion by sublimation/condensation rather than the transport of icy particles by the wind.</p> <p><strong>Mass transfer hypothesis: </strong>In a recent theoretical model [5] scaling laws explain the formation of sublimation waves and have been validated on terrestrial bedforms and on the Martian North Polar Cap. These waves are periodic and oriented in accordance with a turbulent boundary layer flow that diffuse the sublimated vapor. These laws, which are superimposed on the two ice waves studied, make these waves suitable geomorphological markers for climatic predictions. This model has been adapted to study similar waves created by condensation [9] and results indicate that condensation waves would be larger with one order gap than sublimation waves.</p> <p><strong>Discussion: </strong>From the wind tunnel experimentation of ice particles transport in Martian-Like environment [8], wind necessary to initiate transport of ice particles is unlikely in Louth. Friction velocities of 3 m.s<sup>-1</sup> would be required, compared to 0.6 m.s<sup>-1</sup> deduced from scaling laws, to transport the most mobilizable 0.3 mm ice particles [8]. Wind speed in Louth is too weak for initiating particles transport. From the scaling laws, we extract values of friction velocity and flow velocity at a given altitude that can be compared with those extracted from the Martian Data Base over two Martian periods: a period of sublimation (during the summer in the northern hemisphere) at the origin of the formation of decametric waves and a period of condensation at the end of the summer, beginning of the autumn favorable for the kilometric waves formation. Scaling laws are in agreement with the predicted ratio velocity between sublimation and condensation period from the Martian Data Base values. From the Martian Data Base, independent velocity values are larger. This can be explained by the gridding of the database which takes place on a global scale larger than the crater.</p> <p><strong>Conclusion:</strong> The observations indicate kilometric bedforms on the ice cap of Louth Crater, on which decametric bedforms appear with an order of magnitude gap. Comparison with numerical results suggests that the kilometric bedforms are formed by condensation and the smaller ones by sublimation. These condensation and sublimation waves are suitable markers to constrain mesoscale climate modelling in small, complex regions such as this type of crater, where topographic and/or seasonal effects can affect climate data. These waves can also be used as geomarkers on other planetary bodies where climatic conditions are not well constrained. </p> <p><strong>Acknowledgments:</strong> We acknowledge PNP (Plan National de Planétologie) for founding the project “sublimation and condensation waves”. We used Planetary Data System (PDS) for observational data.</p> <p><strong>References:</strong> [1] Conway et al. (2012) <em>Icarus</em>. [2] Brown et al. (2008) <em>Icarus</em>. [3] Hofstader & Murray (1990) <em>Icarus</em>. [4] Appéré et al. (2011) <em>Journal of Geophysical Research.</em> [5] Bordiec M. et al. (2020) <em>Earth-Science Reviews, 211,</em> 103350.[6] Forget et al. (1999). [7] Millour et al. (2018). [8] Herny et al. (2020). <em>7th Mars Polar Science Conf</em>.  Abstract #2099. [9] Carpy S. et al. (2022). <em>EGU</em>, Abstract #5998.</p&gt

ABO Blood Groups in Systemic Sclerosis: Distribution and Association with This Disease’s Characteristics

Systemic sclerosis (SSc) is an autoimmune disease associated with endothelial activation and fibrosis. Non-O blood group patients carry an increased risk of thrombosis, fibrosis and autoimmune diseases. The aim of our work was to evaluate the distribution of ABO groups in SSc patients and their association with the disease’s characteristics. ABO groups were determined in 504 SSc patients (with 131 completed by a genotypic analysis). The distribution of ABO groups and their diplotypes in SSc patients was comparable to that of the general population, except for haplotypes O1 and B (65.6% vs. 61.6% and 8.8% vs. 5.8% in SSc patients vs. the general population, respectively, p = 0.01). The frequency of interstitial lung disease, pulmonary hypertension, calcinosis, digital ulcers, digestive diseases and venous thrombosis, and the Medsger score, were higher in non-O than in O-SSc patients, although they did not display statistical significance. Patients in the non-O group had higher levels of inflammation and endothelial activation biomarkers. In conclusion, the ABO blood group distribution of SSc patients did not differ significantly from that of the general population, but non-O blood groups were associated with inflammation and endothelial activation, and with a non-significant higher frequency of pulmonary and vascular complications in SSc

Crystal structure determination of Pseudomonas stutzeri A1501 endoglucanase Cel5A: the search for a molecular basis for glycosynthesis in GH5_5 enzymes.

The discovery of new glycoside hydrolases that can be utilized in the chemoenzymatic synthesis of carbohydrates has emerged as a promising approach for various biotechnological processes. In this study, recombinant Ps_Cel5A from Pseudomonas stutzeri A1501, a novel member of the GH5_5 subfamily, was expressed, purified and crystallized. Preliminary experiments confirmed the ability of Ps_Cel5A to catalyze transglycosylation with cellotriose as a substrate. The crystal structure revealed several structural determinants in and around the positive subsites, providing a molecular basis for a better understanding of the mechanisms that promote and favour synthesis rather than hydrolysis. In the positive subsites, two nonconserved positively charged residues (Arg178 and Lys216) were found to interact with cellobiose. This adaptation has also been reported for transglycosylating β-mannanases of the GH5_7 subfamily.SCOPUS: ar.jinfo:eu-repo/semantics/publishe

Intravenous immunoglobulins improve skin fibrosis in experimental models of systemic sclerosis

Abstract Systemic sclerosis (SSc) is the most severe systemic autoimmune disease with currently no cure. Intravenous immunoglobulins (IVIg) are an attractive candidate in this disease to counteract inflammation and fibrosis but data are scarce and conflicting. This study, assessed the effects of IVIg in a murine HOCl-induced model of SSc. We showed that IVIg prevented skin inflammation and fibrosis, by mitigating the immune cell infiltration (p = 0.04), proinflammatory cytokines gene overexpression (IL1β, p = 0.04; TNFα, p = 0.04; IL6, p = 0.05), skin and dermal thickening (p = 0.003 at d21 and p = 0.0003 at d42), the expression markers of fibrosis, such as αSMA (p = 0.031 for mRNA and p = 0.05 for protein), collagen (p = 0.05 for mRNA and p = 0.04 for protein, p = 0.05 for the hydroxyproline content) and fibronectin (p = 0.033 for mRNA). Moreover, IVIg prevented HOCl-induced alterations in splenic cell homeostasis. When administered in curative mode, despite their ability to reduce skin and dermal thickness (p < 0.0001 and p = 0.0002), IVIg showed partial or more mixed effects on skin inflammation and established fibrosis. These data favor further clinical trials in SSc patients on the potential efficiency of early and/or repeated IVIg administration

Simple gene signature to assess murine fibroblast polarization

International audienceAbstract We provide an original multi-stage approach identifying a gene signature to assess murine fibroblast polarization. Prototypic polarizations (inflammatory/fibrotic) were induced by seeded mouse embryonic fibroblasts (MEFs) with TNFα or TGFß1, respectively. The transcriptomic and proteomic profiles were obtained by RNA microarray and LC-MS/MS. Gene Ontology and pathways analysis were performed among the differentially expressed genes (DEGs) and proteins (DEPs). Balb/c mice underwent daily intradermal injections of HOCl (or PBS) as an experimental murine model of inflammation-mediated fibrosis in a time-dependent manner. As results, 1456 and 2215 DEGs, and 289 and 233 DEPs were respectively found in MEFs in response to TNFα or TGFß1, respectively . Among the most significant pathways, we combined 26 representative genes to encompass the proinflammatory and profibrotic polarizations of fibroblasts. Based on principal component analysis, this signature deciphered baseline state, proinflammatory polarization, and profibrotic polarization as accurately as RNA microarray and LC-MS/MS did. Then, we assessed the gene signature on dermal fibroblasts isolated from the experimental murine model. We observed a proinflammatory polarization at day 7, and a mixture of a proinflammatory and profibrotic polarizations at day 42 in line with histological findings. Our approach provides a small-size and convenient gene signature to assess murine fibroblast polarization

Development and Validation of a Cytogenetic Prognostic Index Predicting Survival in Multiple Myeloma

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Improved survival in multiple myeloma during the 2005-2009 and 2010-2014 periods

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