7 research outputs found
Lipid Nanostructured Carriers Systems For Ivermectin And Methoprene Aiming Parasite Control
The study of characteristics of nanoscale structures and applications now has great interest of researchers from different areas. In this regard, lipid carriers can improve the bioavailability of drugs and the reduction of possible toxicological effects. Thus, the development of nanostructured systems for the agricultural sector, aimed at combating parasites may reduce extensive damage caused to livestock producers and animal health. This study aimed to prepare and characterize lipid carrier systems, such as Solid Lipid Nanoparticles (SLN) and Nanostructured Lipid Carriers (NLC), as well as evaluate the potential of cyto and genotoxicity of these systems in order to improve and produce alternatives to the use targeting these compounds in veterinary applications. Lipid nanoparticles had become spherical with an average size of 250 nm and remained stable over 120 days. The encapsulation efficiency was greater than 99% for both drugs and release tests showed a strong interaction between drugs and nanoparticles. Cyto- and genotoxicity tests show that nanoparticles made possible changes in cellular viability of the tested cells. Thus, the results showed that the nanoparticles had good colloidal characteristics without changes in its characteristics, enabling the development of carrier systems for these drugs , aiming veterinary applications.3991034104
Lipid nanostructured carriers systems for ivermectin and methoprene aiming parasite control
The study of characteristics of nanoscale structures and applications now has great interest of researchers from different areas. In this regard, lipid carriers can improve the bioavailability of drugs and the reduction of possible toxicological effects. Thus, the development of nanostructured systems for the agricultural sector, aimed at combating parasites may reduce extensive damage caused to livestock producers and animal health. This study aimed to prepare and characterize lipid carrier systems, such as Solid Lipid Nanoparticles (SLN) and Nanostructured Lipid Carriers (NLC), as well as evaluate the potential of cyto and genotoxicity of these systems in order to improve and produce alternatives to the use targeting these compounds in veterinary applications. Lipid nanoparticles had become spherical with an average size of 250 nm and remained stable over 120 days. The encapsulation efficiency was greater than 99% for both drugs and release tests showed a strong interaction between drugs and nanoparticles. Cyto- and genotoxicity tests show that nanoparticles made possible changes in cellular viability of the tested cells. Thus, the results showed that the nanoparticles had good colloidal characteristics without changes in its characteristics, enabling the development of carrier systems for these drugs , aiming veterinary applications39910341043FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULO - FAPESP#2013/12322-2; #2015/15617-9; #2013/24788-
Nanopartículas lipídicas como sistemas carreadores para ivermectina e metopreno visando aplicações em veterinária
O estudo de características e aplicações de estruturas em escala nanométrica passou a ter um grande interesse de pesquisadores das áreas médicas (humana e animal), ambiental e agrícola. Entre essas estruturas, destacam-se os nanocarreadores, que permitiram a melhoraria da biodisponibilidade de muitos compostos bioativos e a diminuição de possíveis efeitos toxicológicos. Além disso, evitar a ocorrência de altos prejuízos na agropecuária com o desenvolvimento de novos nanocarreadores voltados ao combate de endo e ectoparasitas causadores de grandes prejuízos aos pecuaristas. Fármacos como a Ivermectina e o Metopreno são utilizados no combate de ectoparasitas, no entanto, estes compostos possuem problemas relacionados à biodisponibilidade. Este trabalho teve como objetivo preparar e caracterizar sistemas carreadores lipídicos, como as Nanopartículas Lipídicas Sólidas e os Carreadores Lipídicos Nanoestruturados a fim de melhorar e produzir alternativas para o uso destes compostos visando aplicações em veterinária. Na primeira etapa do trabalho foram preparadas e caracterizadas as nanopartículas lipídicas sólidas e os carreadores lipídicos nanoestruturados contendo os fármacos ivermectina e metopreno. As propriedades físico-químicas das suspensões coloidais, como, diâmetro, polidispersão, potencial zeta, pH e eficiência de encapsulação foram avaliadas em função do tempo. A eficiência de encapsulação alcançada foi acima de 99% para ambos os fármacos, sendo que as suspensões coloidais apresentaram estabilidade durante o período investigado. Os resultados do ensaio de citotoxicidade e genotoxicidade demonstram que as nanopartículas testadas para a encapsulação possibilitaram um aumento na viabilidade celular das células testadas, onde as nanopartículas lipídicas sólidas apresentaram menor toxicidade do que os carreadores lipídicos nanoestruturados. As nanopartículas lipídicas sólidas apresentaram tamanho médio de 285,6 ± 15,8 nm e os carreadores lipídicos nanoestruturados de 257,5 ± 7,4 nm. Todas os carreadores apresentaram morfologia esférica, sem formação de agregados e sem alterações morfológicas após a encapsulação. Portanto, os resultados deste trabalho demostraram que as nanopartículas apresentaram boas características coloidais e a presença ou ausência de drogas não provocam alterações nas características das nanopartículas, possibilitando o desenvolvimento de sistemas carreadores para estas drogas visando aplicações veterinárias.The study characteristics and structures of applications in nanoscale have a great interest of researchers in medical areas (human and animal), environment and agriculture. Among these, the nanocarriers stand out, that allowed improve the bioavailability of many bioactive compounds and the reduction of potential toxicological effects, and prevent the occurrence of high losses in agriculture with the development of new nanocarriers aimed at fighting and endo ectoparasites causing great harm to farmers. Drugs such as Ivermectin and Methoprene are used to combat ectoparasites, however, these compounds possess bioavailability problems related. This study aimed to prepare and characterize lipid carrier systems, such as Solid Lipid Nanoparticles and Nanostructured Lipid carriers to improve and produce alternatives to the use of these compounds targeting applications in veterinary. In the first stage of the work we have been prepared and characterized the solid lipid nanoparticles and nanostructured lipid carriers containing the drug ivermectin or methoprene. The physico-chemical properties of colloidal suspensions such as, diameter, polydispersity, Zeta potential, pH and encapsulation efficiency were measured as a function of time. The encapsulation efficiency was achieved above 99% for both drugs, and colloidal suspensions were stable during the period investigated. The results of the cytotoxicity and genotoxicity test demonstrate that the tested nanoparticles for encapsulating allowed an increase in cell viability of test cells, where the solid lipid nanoparticles had lower toxicity than the nanostructured lipid carriers. The solid lipid nanoparticles have an average size of 285,6 ± 15,8 nm and nanostructured lipid carriers 257,5 ± 7,4 nm. All the carriers had spherical morphology without forming aggregates and without morphological changes after encapsulation. Therefore, the results of this study demonstrated that the colloidal nanoparticles showed good characteristics and the presence or absence of drug do not cause changes in the characteristics of the nanoparticles, allowing the development of carrier systems for drug targeting these veterinary applications.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP
SISTEMAS CARREADORES LIPÍDICOS NANOESTRUTURADOS PARA IVERMECTINA E METOPRENO VISANDO CONTROLE DE PARASITAS
The study of characteristics of nanoscale structures and applications now has great interest of researchers from different areas. In this regard, lipid carriers can improve the bioavailability of drugs and the reduction of possible toxicological effects. Thus, the development of nanostructured systems for the agricultural sector, aimed at combating parasites may reduce extensive damage caused to livestock producers and animal health. This study aimed to prepare and characterize lipid carrier systems, such as Solid Lipid Nanoparticles (SLN) and Nanostructured Lipid Carriers (NLC), as well as evaluate the potential of cyto and genotoxicity of these systems in order to improve and produce alternatives to the use targeting these compounds in veterinary applications. Lipid nanoparticles had become spherical with an average size of 250 nm and remained stable over 120 days. The encapsulation efficiency was greater than 99% for both drugs and release tests showed a strong interaction between drugs and nanoparticles. Cyto- and genotoxicity tests show that nanoparticles made possible changes in cellular viability of the tested cells. Thus, the results showed that the nanoparticles had good colloidal characteristics without changes in its characteristics, enabling the development of carrier systems for these drugs , aiming veterinary applications