Analysis of foetal expression sites of human type II DNA topoisomerase α and β mRNAs by in situ hybridisation

We present the first analysis of the sites of expression of DNA topoisomerase IIα and IIβ mRNAs in human foetal tissues by in situ hybridisation, using 35S-radiolabelled probes. This revealed differential localisation of topoisomerase IIα and IIβ mRNAs in a range of foetal organs, including foetal kidney (developing structures within the neogenic zone), brain (cortical layers), small intestine (crypt epithelium and muscle), liver (hepatocytes), lung (smooth muscle, and epithelium in the lining of primitive lung buds) and placenta (trophoblastic epithelium). The intensity of expression of topoisomerase IIα mRNA appeared higher than that of topoisomerase IIβ, although topoisomerase IIβ was expressed in a broader range of cell types. The distinct patterns of expression of topoisomerase IIα and β mRNAs indicate differential regulation of these genes, suggesting that the two isoforms may play important but different roles in foetal development, with topoisomerase IIα being expressed most strongly in zones of proliferation

PyOmeroUpload: A Python toolkit for uploading images and metadata to OMERO

A Python toolkit for automatically extracting metadata from experiment logs and text files, processing images and uploading these payloads to OMERO servers to create fully annotated, multidimensional datasets. The toolkit comes packaged in portable, platform-independent Docker images that enable users to deploy and run the utilities easily, regardless of Operating System constraints. A selection of use cases is provided, illustrating the primary capabilities and flexibility offered with the toolkit, along with a discussion of limitations and potential future extensions. PyOmeroUpload is available from: https://github.com/SynthSys/pyOmeroUpload.Hay J, Troup E, Clark I et al. PyOmeroUpload: A Python toolkit for uploading images and metadata to OMERO. PyOmeroUpload is available from: https://github.com/SynthSys/pyOmeroUpload. Archived source code at time of publication: https://doi.org/10.5281/zenodo.374648640