136 research outputs found
Population-based laboratory surveillance of Hafnia alvei isolates in a large Canadian health region
BACKGROUND: Hospital-based series have characterized Hafnia alvei primarily as an infrequent agent of polymicrobial nosocomial infections in males with underlying illness. METHODS: We conducted population-based laboratory surveillance in the Calgary Health Region during 2000–2005 to define the incidence, demographic risk factors for acquisition, and anti-microbial susceptibilities of Hafnia alvei isolates. RESULTS: A total of 138 patients with Hafnia alvei isolates were identified (2.1/100,000/year) and two-thirds were of community onset. Older age and female gender were important risk factors for acquisition. The most common focus of isolation was urine in 112 (81%), followed by lower respiratory tract in 10 (7%), and soft tissue in 5 (4%), and the majority (94; 68%) were mono-microbial. Most isolates were resistant to ampicillin (111;80%), cephalothin (106; 77%), amoxicillin/clavulanate (98; 71%), and cefazolin (95; 69%) but none to imipenem or ciprofloxacin. CONCLUSION: Hafnia alvei was most commonly isolated as a mono-microbial etiology from the urinary tract in women from the community. This study highlights the importance of population-based studies in accurately defining the epidemiology of an infectious disease
Population-based Laboratory Surveillance for AmpC β-Lactamase–producing Escherichia coli, Calgary
AmpC β-lactamase–producing E. coli are commonly isolated from the urinary tract of older women
Tackling antimicrobial resistance in lower urinary tract infections : treatment options
Urinary tract infections (UTIs) are among the most common infectious diseases occurring in
either the community or healthcare settings. A wide variety of bacteria are responsible for
causing UTIs, however extra-intestinal pathogenic E. coli or ExPEC) remains the most common
etiological agent. Since 2000, resistance to antibiotics emerged globally among ExPEC and is
causing delays in appropriate therapy with subsequent increased morbidity and mortality. For
patients with acute uncomplicated lower UTIs, nitrofurantoin, trimethoprim-sulfamethoxazole,
fosfomycin or pivmecillinam should be prescribed for a 1-5 day course depending on the agent
used. Single-dose fosfomycin is an excellent option for uncomplicated lower UTIs and has had
similar clinical and/or bacteriological efficacy for 3- or 7-day regimens for alternate agents (i.e.,
ciprofloxacin, norfloxacin, cotrimoxazole or nitrofurantoin). The aim of this review article is to
provide an overview on the definitions, etiology, treatment guidelines (including agents for infections due to antimicrobial resistant bacteria) of lower UTIs and to highlight recent aspects
on antimicrobial resistance of ExPEC.In part by a research grant from Calgary Laboratory Services
(#10009392).http://www.tandfonline.com/loi/ierz202017-07-30hb2016Medical Microbiolog
Compartmentalization of the gut viral reservoir in HIV-1 infected patients
<p>Abstract</p> <p>Background</p> <p>Recently there has been an increasing interest and appreciation for the gut as both a viral reservoir as well as an important host-pathogen interface in human immunodefiency virus type 1 (HIV-1) infection. The gut associated lymphoid tissue (GALT) is the largest lymphoid organ infected by HIV-1. In this study we examined if different HIV-1 quasispecies are found in different parts of the gut of HIV-1 infected individuals.</p> <p>Results</p> <p>Gut biopsies (esophagus, stomach, duodenum and colorectum) were obtained from eight HIV-1 infected preHAART (highly active antiretroviral therapy) patients. HIV-1 Nef and Reverse transcriptase (RT) encoding sequences were obtained through nested PCR amplification from DNA isolated from the gut biopsy tissues. The PCR fragments were cloned and sequenced. The resulting sequences were subjected to various phylogenetic analyses. Expression of the <it>nef </it>gene and viral RNA in the different gut tissues was determined using real-time RT-PCR. Phylogenetic analysis of the Nef protein-encoding region revealed compartmentalization of viral replication in the gut within patients. Viral diversity in both the Nef and RT encoding region varied in different parts of the gut. Moreover, increased <it>nef </it>gene expression (p < 0.05) and higher levels of viral genome were observed in the colorectum (p < 0.05). These differences could reflect an adaptation of HIV-1 to the various tissues.</p> <p>Conclusion</p> <p>Our results indicated that different HIV-1 quasispecies populate different parts of the gut, and that viral replication in the gut is compartmentalized. These observations underscore the importance of the gut as a host-pathogen interface in HIV-1 infection.</p
Intensive care unit-acquired urinary tract infections in a regional critical care system
INTRODUCTION: Few studies have evaluated urinary tract infections (UTIs) specifically acquired within intensive care units (ICUs), and the effect of such infections on patient outcome is unclear. The objectives of this study were to describe the occurrence, microbiology, and risk factors for acquiring UTIs in the ICU and to determine whether these infections independently increase mortality. METHODS: A surveillance cohort study was conducted among all adults admitted to multi-system and cardiovascular surgery ICUs in the Calgary Health Region (CHR, population about 1 million) between 1 January 2000 and 31 December 2002. RESULTS: During the 3 years, 4465 patients were admitted 4915 times to a CHR ICU for 48 hours or more. A total of 356 ICU-acquired UTIs (defined as at least 10(5 )colony-forming units/ml of one or two organisms 48 hours or more after ICU admission) occurred among 290 (6.5%) patients, yielding an overall incidence density of ICU-acquired UTIs of 9.6 per 1000 ICU days. Four bacteremic/fungemic ICU-acquired UTIs occurred (0.1 per 1000 ICU days). Development of an ICU-acquired UTI was more common in women (relative risk [RR] 1.58; 95% confidence interval [CI] 1.43–1.75; P < 0.0001) and in medical (9%) compared with non-cardiac surgical (6%), and cardiac surgical patients (2%). The most common organisms isolated were Escherichia coli (23%), Candida albicans (20%), and Enterococcus species (15%). Antibiotic-resistant organisms were identified among 14% isolates. Although development of an ICU-acquired UTI was associated with significantly higher crude in-hospital mortality (86/290 [30%] vs. 862/4167 [21%]; RR = 1.43; 95% CI 1.19–1.73; P < 0.001); an ICU-acquired UTI was not an independent predictor for death. CONCLUSIONS: Development of an ICU-acquired UTI is common in critically ill patients. Although a marker of increased morbidity associated with critical illness, it is not a significant attributable cause of mortality
Colistin non-susceptible Pseudomonas aeruginosa ST654 with blaNDM-1 arrives in North America
This study describes 3 different blaNDM-1 genetic platforms in 3 different species obtained from
the same patient who was directly transferred to an institution in Calgary, Canada, following a
prolonged hospital stay in India. The blaNDM-1 in the Escherichia coli was located on a 176kb
IncA/C plasmid contained within an ISCR1 region. The blaNDM-1 in the Providencia rettgeri was
located on a 117kb IncT plasmid contained within Tn3000, while the blaNDM-1 in Pseudomonas
aeruginosa was located on the chromosome within an ISCR3 region. This report highlights the
plasticity of the genetic regions and environments associated with blaNDM-1. To the best of our
knowledge, this is the first report of P. aeruginosa with blaNDM-1 identified in North America and
the first report of blaOXA-181in P. rettgeri. The P. aeruginosa belonged to the international high
risk clone ST654 and was non-susceptible to colistin. This case emphasizes the need for
appropriate infection prevention and control measures and vigilant screening for carbapenem
resistant Gram negative bacteria in patients with a history of travel to endemic areas, such as the
Indian subcontinent.In part by a research grant from the Calgary Laboratory Services (#10009392).http://aac.asm.org2016-09-30hb201
Rationale for and protocol of a multi-national population-based bacteremia surveillance collaborative
<p>Abstract</p> <p>Background</p> <p>Bloodstream infections are frequent causes of human illness and cause major morbidity and death. In order to best define the epidemiology of these infections and to track changes in occurrence, adverse outcome, and resistance rates over time, population based methodologies are optimal. However, few population-based surveillance systems exist worldwide, and because of differences in methodology inter-regional comparisons are limited. In this report we describe the rationale and propose first practical steps for developing an international collaborative approach to the epidemiologic study and surveillance for bacteremia.</p> <p>Findings</p> <p>The founding collaborative participants represent six regions in four countries in three continents with a combined annual surveillance population of more than 8 million residents.</p> <p>Conclusion</p> <p>Future studies from this collaborative should lead to a better understanding of the epidemiology of bloodstream infections.</p
Molecular characterization by using next generation sequencing of plasmids containing blaNDM-7 in Enterobacteriaceae from Calgary, Canada
Enterobacteriaceae with blaNDM-7 is relatively uncommon and had previously been described in
Europe, India, USA and Japan. This study describes the characteristics of Enterobacteriaceae
[Klebsiella pneumoniae (n=2), Escherichia coli (n=2), Serratia marcescens (n=1), Enterobacter
hormaechei (n=1)] with blaNDM-7 obtained in 4 patients from Calgary, Canada during 2013-4.
The 46,161 bp IncX3 plasmids with blaNDM-7 are highly similar to other blaNDM-harboring IncX3
plasmids and interestingly, showed identical structures within the different isolates. This finding
may indicate horizontal transmission within our health region or may indicate contact with
individuals from endemic areas within the hospital setting. Patients infected or colonized with
bacteria containing blaNDM-7 IncX3 plasmids will generate infection control challenges.
Epidemiological and molecular studies are required to better understand the dynamics of
transmission, risk factors and reservoirs for bacteria harboring blaNDM-7. To the best of our
knowledge, this is the first report of S. marcescens, and E. hormaechei with blaNDM-7.Calgary Laboratory Services (#10006465) and in part by a grant (to B.N.K.) from the National Institutes of Health (1R01AI090155).http://aac.asm.org2016-09-30hb201
Human Case of Lobomycosis
We describe a 42-year-old woman with histologically confirmed lobomycosis, a cutaneous fungal infection rarely reported outside of Latin America. Our case represents the first published report of imported human lobomycosis in Canada and the fifth in an industrialized country
Blood cultures in ambulatory outpatients
BACKGROUND: Blood cultures are a gold standard specific test for diagnosing many infections. However, the low yield may limit their usefulness, particularly in low-risk populations. This study was conducted to assess the utility of blood cultures drawn from ambulatory outpatients. METHODS: Blood cultures drawn at community-based collection sites in the Calgary Health Region (population 1 million) in 2001 and 2002 were included in this study. These patients were analyzed by linkages to acute care health care databases for utilization of acute care facilities within 2 weeks of blood culture draw. RESULTS: 3102 sets of cultures were drawn from 1732 ambulatory outpatients (annual rate = 89.4 per 100,000 population). Significant isolates were identified from 73 (2.4%) sets of cultures from 51 patients, including Escherichia coli in 18 (35%) and seven (14%) each of Staphylococcus aureus and Streptococcus pneumoniae. Compared to patients with negative cultures, those with positive cultures were older (mean 49.6 vs. 40.1 years, p < 0.01), and more likely to subsequently receive care at a regional emergency department, outpatient antibiotic clinic, or hospital (35/51 vs. 296/1681, p < 0.0001). Of the 331 (19%) patients who received acute care treatment, those with positive cultures presented sooner after community culture draw (median 2 vs. 3 days, p < 0.01) and had longer median treatment duration (6 vs. 2 days, p < 0.01). CONCLUSION: Blood cultures drawn in outpatient settings are uncommonly positive, but may define patients for increased intensity of therapy. Strategies to reduce utilization without excluding patients with positive cultures need to be developed for this patient population
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