DMBA induced mammary tumor study of Phyto-fabricated sliver nanoparticles from Russelia Equisetiformis flower extract in rat

The mammary tumor is the world's most frequent ailment, and it is substantially more common in women. There are several forms of treatment and care available in the globe for tumors, the most common of which being chemotherapy, radiation therapy, and surgery with lots of harmful responses. Cancer has been treated since ancient times by herbs with various herbs and metals. synthesized AgNPs-REEE were characterized by Analytical methods such as dynamic light scattering (DLS), Zeta potential, scanning electron microscopy (SEM), energy dispersive spectroscopy (EDX) and Dynamic Light Scattering (DLS).All of the animals in this study had tumors caused by DMBA Apart from the vehicle control group, and they were divided into different groups such as only DMBA(7,12-dimethylbenz[a]anthracene) induced group, treatment group of ethanolic extract of Russelia equisetiformis (REEE) flower and sliver nanoparticles of ethanolic extract of Russelia equisetiformis (REEE) flower and sliver nanoparticles of ethanolic extract (AgNPs -REEE).the dose were decide by acute toxicity study. After compaction treatment time all animal were sacrifices and evaluate parameter like tumor weight, Tumor volume, biochemical parameter, hormonal assay and histopathology of breast tumor. It is observed that the size distribution of AgNPs ranges from 50 to 950 nm.&nbsp

Hepatoprotective Effects of Thespesia lampas Dalz & Gibs in CCl4 Induced Liver Injury in Rats

ABSTRACT: The extracts of the roots of Thespesia lampas (Malvaceae) were evaluated for hepatoprotective activity in rats by inducing chronic liver damage by subcutaneous injection of 50 % v/v carbon tetrachloride in Tween 80 at a dose of 3ml/kg for a period of 4 weeks. The biochemical parameters like serum glutamate pyruvate transaminase (SGPT), serum glutamate oxaloacetate transaminase (SGOT), alkaline phosphatase (ALP), serum bilirubin and total proteins were estimated to assess the liver function. Hepatic steatosis, centrilobular necrosis, and often swelling of the hepatic cytoplasm were observed in carbontetra chloride treated group, while these were completely absent in the extracts of T. lampas (300 mg/kg b.wt) treated groups (p<0.01). The present investigation established pharmacological evidence to support the folkloric claim of hepatoprotective activity of T. lampas

Synthesis, analgesic and anti-inflammatory activities of some pyrazolo[3,4-c]pyrazole derivatives

Historically, heterocyclic compounds containing Nitrogen, and their derivatives have been invaluable as a source of therapeutic agents. Pyrazole, with two nitrogen atoms and aromatic character, provides diverse functionality and stereochemical complexity in a five-membered ring structure. In Knorr pyrazole synthesis, diimine compound gets deprotonated to regenerate the acid catalyst and provide the final pyrazole product. Formation of pyrazole derivatives from hydrazines, hydrazides, semicarbazides, thiosemicarbazide and aminoguanidines by condensation with 1,3-dicarbonyl compounds is possible. As fused pyrazoles are reported to be well known pharmacophores, this has motivated to synthesize some of the pyrazolopyrazole derivatives by using hydrazine hydrate, thiosemicarbazide and semicarbazide. A series of 3-(aryl)-4-methyl-3a,6-dihydropyrazolo[3,4-c]pyrazole-2(3H)-carboxamides (IVa3-e3), 3-(aryl)-4-methyl-3a,6-dihydropyrazolo[3,4-c]pyrazole-2(3H)-carbothioamide (IVa2-e2) and 4-(aryl)-3-methyl-1,3a,4,5-tetrahydropyrazolo[3,4-c]pyrazoles (IVa1-e1) were synthesized by conventional method where fused pyrazopyrazoles were prepared.  All the compounds were synthesized with good yield (56-81 %) and characterized by IR, 1H NMR spectral data and C, H, N elemental analysis. All the synthesized compounds exhibited analgesic and anti-inflammatory activities

Development and validation of stability indicating UV-visible spectrophotometric method for simultaneous determination of salbutamol sulphate and ambroxol hydrochloridein liquid dosage form

Purpose: A new, simple, precise and validated UV spectrophotometric method has been developed for simultaneous estimation of salbutamol sulphate and ambroxol hydrochloride in liquid dosage form. Method: The method employed was simultaneous equation method which involves solving simultaneous equations based on measurement of absorbance at two wavelengths 224 nm and 244 nm, the λmax of salbutamol sulphate and ambroxol hydrochloride, respectively. Specificity of the method was determined by subjecting the drugs to various stress conditions like acid, alkali, thermal and photolytic degradation. Results: The linearity was obtained in the concentration range of 0.1-0.6 μg/mL for salbutamol sulphateand 1.5-09 μg/mL for ambroxol hydrochloride. The proposed method was effectively applied to liquid dosage form for estimation of both drugs. The accuracy and reproducibility results are close to 100% with ≤2% RSD. The results of proposed method have been validated as per ICH guidelines. Conclusion: A novel, precise and accurate stability indicating spectrophotometric method has been developed for the estimation of salbutamol sulphate and ambroxol hydrochloride in pharmaceutical syrup formulation

Development and Validation of Stability Indicating UV-visible Spectrophotometric Method for Simultaneous Determination of Salbutamol Sulphate and Ambroxol Hydrochloridein Liquid Dosage Form

Purpose: A new, simple, precise and validated UV spectrophotometric method has been developed for simultaneous estimation of salbutamol sulphate and ambroxol hydrochloride in liquid dosage form. Method: The method employed was simultaneous equation method which involves solving simultaneous equations based on measurement of absorbance at two wavelengths 224 nm and 244 nm, the λmax of salbutamol sulphate and ambroxol hydrochloride, respectively. Specificity of the method was determined by subjecting the drugs to various stress conditions like acid, alkali, thermal and photolytic degradation. Results: The linearity was obtained in the concentration range of 0.1-0.6 μg/mL for salbutamol sulphateand 1.5-09 μg/mL for ambroxol hydrochloride. The proposed method was effectively applied to liquid dosage form for estimation of both drugs. The accuracy and reproducibility results are close to 100% with ≤2% RSD. The results of proposed method have been validated as per ICH guidelines. Conclusion: A novel, precise and accurate stability indicating spectrophotometric method has been developed for the estimation of salbutamol sulphate and ambroxol hydrochloride in pharmaceutical syrup formulation