Determination of optimal NH4+/K + concentration and corresponding ratio critical for growth of tobacco seedlings in a hydroponic system

Inherently, ammonium (NH4+) is critical for plant growth; however, its toxicity suppresses potassium (K+) uptake and vice-versa. Hence, attaining a nutritional balance between these two ions (NH4+ and K+) becomes imperative for the growth of tobacco seedlings. Therefore, we conducted a 15-day experimental study on tobacco seedlings exposed to different concentrations (47 treatments) of NH4+/K+ at different corresponding 12 ratios simultaneously in a hydroponic system. Our study aimed at establishing the optimal NH4+-K+ concentration and the corresponding ratio required for optimal growth of different tobacco plant organs during the seedling stage. The controls were the baseline for comparison in this study. Plants with low or excessive NH4+-K+ concentration had leaf chlorosis or dark greenish colouration, stunted whole plant part biomass, and thin roots. We found that adequate K+ supply is a pragmatic way to mitigate NH4+-induced toxicity in tobacco plants. The optimal growth for tobacco leaf and root was attained at NH4+-K+ concentrations 2-2 mM (ratio 1:1), whereas stem growth was optimal at NH4+-K+ 1-2 mM (1:2). The study provided an insight into the right combination of NH4+/K+ that could mitigate or prevent NH4+ or K+ stress in the tobacco seedlings

Effect of a family-involvement combined aerobic and resistance exercise protocol on cancer-related fatigue in patients with breast cancer during postoperative chemotherapy: study protocol for a quasi-randomised controlled trial

Introduction Cancer-related fatigue (CRF) is one of the most common and debilitating side effects experienced by patients with breast cancer (BC) during postoperative chemotherapy. Family-involvement combined aerobic and resistance exercise has been introduced as a promising non-pharmacological intervention for CRF symptom relief and improving patients’ muscle strength, exercise completion, family intimacy and adaptability and quality of life. However, evidence for the practice of home participation in combined aerobic and resistance exercise for the management of CRF in patients with BC is lacking.Methods and analysis We present a protocol for a quasi-randomised controlled trial involving an 8-week intervention. Seventy patients with BC will be recruited from a tertiary care centre in China. Participants from the first oncology department will be assigned to the family-involvement combined aerobic and resistance exercise group (n=28), while participants from the second oncology department will be assigned to the control group that will receive standard exercise guidance (n=28). The primary outcome will be the Piper Fatigue Scale-Revised (R-PFS) score. The secondary outcomes will include muscle strength, exercise completion, family intimacy and adaptability and quality of life, which will be evaluated by the stand-up and sit-down chair test, grip test, exercise completion rate, Family Adaptability and Cohesion Scale, Second Edition-Chinese Version (FACESⅡ-CV) and Functional Assessment of Cancer Therapy -Breast (FACT-B) scale. Analysis of covariance will be applied for comparisons between groups, and paired t-tests will be used for comparison of data before and after exercise within a group.Ethics and dissemination This study has been approved by the Ethics Committee of the First Affiliated Hospital of Dalian Medical University (PJ-KS-KY-2021-288). The results of this study will be published via peer-reviewed publications and presentations at conferences.Trail registration number ChiCTR2200055793

Know it to Defeat it: Exploring Health Rumor Characteristics and Debunking Efforts on Chinese Social Media during COVID-19 Crisis

Health-related rumors spreading online during a public crisis may pose a serious threat to people's well-being. Existing crisis informatics research lacks in-depth insights into the characteristics of health rumors and the efforts to debunk them on social media in a pandemic. To fill this gap, we conduct a comprehensive analysis of four months of rumor-related online discussion during COVID-19 on Weibo, a Chinese microblogging site. Results suggest that the dread (cause fear) type of health rumors provoked significantly more discussions and lasted longer than the wish (raise hope) type. We further explore how four kinds of social media users (i.e., government, media, organization, and individual) combat health rumors, and identify their preferred way of sharing debunking information and the key rhetoric strategies used in the process. We examine the relationship between debunking and rumor discussions using a Granger causality approach, and show the efficacy of debunking in suppressing rumor discussions, which is time-sensitive and varies across rumor types and debunkers. Our results can provide insights into crisis informatics and risk management on social media in pandemic settings.Comment: ICWSM 202

Modelling of crack propagation of gravity dams by scaled boundary polygons and cohesive crack model

Crack propagation in concrete gravity dams is investigated using scaled boundary polygons coupled with interface elements. The concrete bulk is assumed to be linear elastic and is modelled by the scaled boundary polygons. The interface elements model the fracture process zone between the crack faces. The cohesive tractions are modelled as side-face tractions in the scaled boundary polygons. The solution of the stress field around the crack tip is expressed semi-analytically as a power series. It reproduces the singular and higher-order terms in an asymptotic solution, such as the William's eigenfunction expansion when the cohesive tractions vanish. Accurate results can be obtained without asymptotic enrichment or local mesh refinement. The stress intensity factors are obtained directly from their definition and provide a convenient and accurate means to assess the zero-K condition, which determines the stability of a cohesive crack. The direction of crack propagation is determined from the maximum circumferential stress criterion. To accommodate crack propagation, a local remeshing algorithm that is applicable to any polygon mesh is augmented by inserting cohesive interface elements between the crack surfaces as the cracks propagate. Three numerical benchmarks involving crack propagation in concrete gravity dams are modelled. The results are compared to the experimental and other numerical simulations reported in the literature. © 2013 Springer Science+Business Media Dordrecht

Research Progress on Plant Shaker K⁺ Channels

Plant growth and development are driven by intricate processes, with the cell membrane serving as a crucial interface between cells and their external environment. Maintaining balance and signal transduction across the cell membrane is essential for cellular stability and a host of life processes. Ion channels play a critical role in regulating intracellular ion concentrations and potentials. Among these, K+ channels on plant cell membranes are of paramount importance. The research of Shaker K+ channels has become a paradigm in the study of plant ion channels. This study offers a comprehensive overview of advancements in Shaker K+ channels, including insights into protein structure, function, regulatory mechanisms, and research techniques. Investigating Shaker K+ channels has enhanced our understanding of the regulatory mechanisms governing ion absorption and transport in plant cells. This knowledge offers invaluable guidance for enhancing crop yields and improving resistance to environmental stressors. Moreover, an extensive review of research methodologies in Shaker K+ channel studies provides essential reference solutions for researchers, promoting further advancements in ion channel research

Farpls:A Feature-Augmented Robot Trajectory Preference Labeling System to Assist Human Labelers' Preference Elicitation

Preference-based learning aims to align robot task objectives with human values. One of the most common methods to infer human preferences is by pairwise comparisons of robot task trajectories. Traditional comparison-based preference labeling systems seldom support labelers to digest and identify critical differences between complex trajectories recorded in videos. Our formative study (N = 12) suggests that individuals may overlook non-salient task features and establish biased preference criteria during their preference elicitation process because of partial observations. In addition, they may experience mental fatigue when given many pairs to compare, causing their label quality to deteriorate. To mitigate these issues, we propose FARPLS, a Feature-Augmented Robot trajectory Preference Labeling System. FARPLS highlights potential outliers in a wide variety of task features that matter to humans and extracts the corresponding video keyframes for easy review and comparison. It also dynamically adjusts the labeling order according to users' familiarities, difficulties of the trajectory pair, and level of disagreements. At the same time, the system monitors labelers' consistency and provides feedback on labeling progress to keep labelers engaged. A between-subjects study (N = 42, 105 pairs of robot pick-and-place trajectories per person) shows that FARPLS can help users establish preference criteria more easily and notice more relevant details in the presented trajectories than the conventional interface. FARPLS also improves labeling consistency and engagement, mitigating challenges in preference elicitation without raising cognitive loads significantl

Prefrontal cortical dopamine deficit may cause impaired glucose metabolism in schizophrenia

Abstract The brain neurotramsmitter dopamine may play an important role in modulating systemic glucose homeostasis. In seven hundred and four drug- naïve patients with first-episode schizophrenia, we provide robust evidence of positive associations between negative symptoms of schizophrenia and high fasting blood glucose. We then show that glucose metabolism and negative symptoms are improved when intermittent theta burst stimulation (iTBS) on prefrontal cortex (PFC) is performed in patients with predominantly negative symptoms of schizophrenia. These findings led us to hypothesize that the prefrontal cortical dopamine deficit, which is known to be associated with negative symptoms, may be responsible for abnormal glucose metabolism in schizophrenia. To explore this, we optogenetically and chemogenetically inhibited the ventral tegmental area (VTA)-medial prefrontal cortex (mPFC) dopamine projection in mice and found both procedures caused glucose intolerance. Moreover, microinjection of dopamine two receptor (D2R) neuron antagonists into mPFC in mice significantly impaired glucose tolerance. Finally, a transgenic mouse model of psychosis named Disc1tr exhibited depressive-like symptoms, impaired glucose homeostasis, and compared to wild type littermates reduced D2R expression in prefrontal cortex

DataSheet_1_Genome-wide identification of Shaker K+ channel family in Nicotiana tabacum and functional analysis of NtSKOR1B in response to salt stress.docx

Soil salinization poses a mounting global ecological and environmental threat. The identification of genes responsible for negative regulation of salt tolerance and their utilization in crop improvement through gene editing technologies emerges as a swift strategy for the effective utilization of saline-alkali lands. One efficient mechanism of plant salt tolerance is maintaining the proper intracellular K+/Na+ ratio. The Shaker K+ channels play a crucial role in potassium absorption, transport, and intracellular potassium homeostasis in plant cells. Here, the study presents the first genome-wide identification of Shaker K+ channels in Nicotiana tabacum L., along with a detailed bioinformatic analysis of the 20 identified members. Transcriptome analysis revealed a significant up-regulation of NtSKOR1B, an outwardly-rectifying member predominantly expressed in the root tissue of tobacco seedlings, in response to salt stress. This finding was then confirmed by GUS staining of ProNtSKOR1B::GUS transgenic lines and RT-qPCR analysis. Subsequently, NtSKOR1B knockout mutants (ntskor1) were then generated and subjected to salt conditions. It was found that ntskor1 mutants exhibit enhanced salt tolerance, characterized by increased biomass, higher K+ content and elevated K+/Na+ ratios in both leaf and root tissues, compared to wild-type plants. These results indicate that NtSKOR1B knockout inhibits K+ efflux in root and leaf tissues of tobacco seedlings under salt stress, thereby maintaining higher K+/Na+ ratios within the cells. Thus, our study identifies NtSKOR1B as a negative regulator of salt tolerance in tobacco seedlings.</p