1,331 research outputs found

    Incorporate Credibility into Context for the Best Social Media Answers

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    A lost decade for third-wave democracies?

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    By comparing results based on expert and popular surveys, this article finds that popular support for democracy was dwindling even in the decade before expert surveys (such as Freedom in the World and V-Dem) began to show the reality of democratic retreat. Decreasing support for democracy is tied to low satisfaction with how democracy works in practice, and it contributes to the rise of populist leaders who concentrate power in the executive branch. These phenomena reflect the waning capacity of the state to improve governance across all countries. As a result, democracy is likely to face another lackluster decade if both structural and institutional problems are left unsolved

    An efficient DNA isolation method for tropical plants

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    Due to interfering components such as polysacharrides, polyphenols, etc, DNA isolation from tropical plants had been challenging. We developed a safe, universal and efficient DNA extraction method, which yielded high-quality DNA from 10 tropical plants including cassava, rubber tree, banana, etc. In the extraction buffer, 2 M NaCl was used to provide a high ionic strength reaction environment, ethylenediaminetetraacetic acid (EDTA), lauroyl sarcosine (LSS) and cetyl trimethyl ammonium bromide (CTAB) could inhibit DNase activity effectively, polyvinylpolypyrrolidone (PVPP) produced a deoxidized reaction environment, and borax enhanced the precipitation of interfering compounds. Ordinary reagents like β-mercaptoethanol, chloroform and phenol were unnecessary in this protocol, which made it safe and friendly to use. PCR and EcoR I enzyme restriction digestion results show that the obtained DNA is good enough for downstream analysis. In conclusion, this protocol is expected to be a preferable DNA extraction protocol for tropical plants.Keywords: DNA extraction, tropical plants, cetyl trimethyl ammonium bromide (CTAB)African Journal of Biotechnology Vol. 12(19), pp. 2727-273

    Does PGA external stenting reduce compliance mismatch in venous grafts?

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    BACKGROUND: Autogenous vein grafting is widely used in regular bypassing procedures. Due to its mismatch with the host artery in both mechanical property and geometry, the graft often over expands under high arterial blood pressure and forms a step-depth where eddy flow develops, thus causing restenosis, fibrous graft wall, etc. External stents, such as sheaths being used to cuff the graft, have been introduced to eliminate these mismatches and increase the patency. Although histological and immunochemical studies have shown some positive effects of the external stent, the mechanical mismatch under the protection of an external stent remains poorly analyzed. METHODS: In this study, the jugular veins taken from hypercholesterolemic rabbits were transplanted into the carotid arteries, and non-woven polyglycolic acid (PGA) fabric was used to fabricate the external stents to study the effect of the biodegradable external stent. Eight weeks after the operation, the grafts were harvested to perform mechanical tests and histological examinations. An arc tangent function was suggested to describe the relationship between pressure and cross-sectional area to analyse the compliance of the graft. RESULTS: The results from the mechanical tests indicated that grafts either with or without external stents displayed large compliance in the low-pressure range and were almost inextensible in the high-pressure range. This was very different from the behavior of the arteries or veins in vivo. The data from histological tests showed that, with external stents, collagen fibers were more compact, whilst those in the graft without protection were looser and thicker. No elastic fiber was found in either kind of grafts. Furthermore, grafts without protection were over-expanded which resulted in much bigger cross-sectional areas. CONCLUSION: The PGA external extent contributes little to the reduction of the mechanical mismatch between the graft and its host artery while remodeling develops. For the geometric mismatch, it reduces the cross-section area, therefore matching with the host artery much better. Although there are some positive effects, conclusively the PGA is not an ideal material for external stent.RIGHTS : This article is licensed under the BioMed Central licence at http://www.biomedcentral.com/about/license which is similar to the 'Creative Commons Attribution Licence'. In brief you may : copy, distribute, and display the work; make derivative works; or make commercial use of the work - under the following conditions: the original author must be given credit; for any reuse or distribution, it must be made clear to others what the license terms of this work are

    Observational connection of non-thermal X-ray emission from pulsars with their timing properties and thermal emission

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    The origin and radiation mechanisms of high energy emissions from pulsars have remained mysterious since their discovery. Here we report, based on a sample of 68 pulsars, observational connection of non-thermal X-ray emissions from pulsars with their timing properties and thermal emissions, which may provide some constraints on theoretical modeling. Besides strong correlations with the spin-down power E˙\dot{E} and the magnetic field strength at the light cylinder BlcB_{\rm lc}, the non-thermal X-ray luminosity in 0.5 - 8 keV, LpL_{\rm p}, represented by the power-law component in the spectral model, is found to be strongly correlated with the highest possible electric field strength in the polar gap, EpcE_{\rm pc}, of the pulsar. The spectral power index Γp\Gamma_{\rm p} of that power-law component is also found, for the first time in the literature, to strongly correlate with E˙\dot{E}, BlcB_{\rm lc} and EpcE_{\rm pc}, thanks to the large sample. In addition, we found that LpL_{\rm p} can be well described by LpT5.96±0.64R2.24±0.18L_{\rm p}\propto T^{5.96\pm 0.64}R^{2.24\pm 0.18}, where TT and RR are the surface temperature and the emitting-region radius of the surface thermal emission, represented by the black-body component in the spectral model. Γp\Gamma_{\rm p}, on the other hand, can be well described only when timing variables are included, and the relation is Γp=log(T5.8±1.93R2.29±0.85P1.19±0.88P˙0.94±0.44)\Gamma_{\rm p} = \log(T^{-5.8\pm 1.93}R^{-2.29\pm 0.85}P^{-1.19\pm 0.88}\dot{P}^{0.94\pm 0.44}) plus a constant. These relations strongly suggest the existence of connections between surface thermal emission and electron-positron pair production in pulsar magnetospheres.Comment: 13 pages, 11 figures, accepted by MNRA
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