Genome Sequencing of the Sweetpotato Whitefly \u3cem\u3eBemisia tabaci\u3c/em\u3e MED/Q

The sweetpotato whitefly Bemisia tabaci is a highly destructive agricultural and ornamental crop pest. It damages host plants through both phloem feeding and vectoring plant pathogens. Introductions of B. tabaci are difficult to quarantine and eradicate because of its high reproductive rates, broad host plant range, and insecticide resistance. A total of 791 Gb of raw DNA sequence from whole genome shotgun sequencing, and 13 BAC pooling libraries were generated by Illumina sequencing using different combinations of mate-pair and pair-end libraries. Assembly gave a final genome with a scaffold N50 of 437 kb, and a total length of 658 Mb. Annotation of repetitive elements and coding regions resulted in 265.0 Mb TEs (40.3%) and 20 786 protein-coding genes with putative gene family expansions, respectively. Phylogenetic analysis based on orthologs across 14 arthropod taxa suggested that MED/Q is clustered into a hemipteran clade containing A. pisum and is a sister lineage to a clade containing both R. prolixus and N. lugens. Genome completeness, as estimated using the CEGMA and Benchmarking Universal Single-Copy Orthologs pipelines, reached 96% and 79%. These MED/Q genomic resources lay a foundation for future \u27pan-genomic\u27 comparisons of invasive vs. noninvasive, invasive vs. invasive, and native vs. exotic Bemisia, which, in return, will open up new avenues of investigation into whitefly biology, evolution, and management

Genomic, Pathway Network, and Immunologic Features Distinguishing Squamous Carcinomas

This integrated, multiplatform PanCancer Atlas study co-mapped and identified distinguishing molecular features of squamous cell carcinomas (SCCs) from five sites associated with smokin

Finishing the euchromatic sequence of the human genome

The sequence of the human genome encodes the genetic instructions for human physiology, as well as rich information about human evolution. In 2001, the International Human Genome Sequencing Consortium reported a draft sequence of the euchromatic portion of the human genome. Since then, the international collaboration has worked to convert this draft into a genome sequence with high accuracy and nearly complete coverage. Here, we report the result of this finishing process. The current genome sequence (Build 35) contains 2.85 billion nucleotides interrupted by only 341 gaps. It covers ∼99% of the euchromatic genome and is accurate to an error rate of ∼1 event per 100,000 bases. Many of the remaining euchromatic gaps are associated with segmental duplications and will require focused work with new methods. The near-complete sequence, the first for a vertebrate, greatly improves the precision of biological analyses of the human genome including studies of gene number, birth and death. Notably, the human enome seems to encode only 20,000-25,000 protein-coding genes. The genome sequence reported here should serve as a firm foundation for biomedical research in the decades ahead

Analysis of the Factors Affecting China’s Manufacturing Servitization from the Perspective of the Ecological Environment

Manufacturing servitization (MS) can effectively alleviate the contradiction between economic growth and ecological carrying capacity and can promote energy conservation and emission reduction in the manufacturing industry (MI). However, China’s MI is in the primary stage of servitization and lacks sufficient ability to provide services; thus, the environmental benefits of MS are not obvious. Therefore, in the context of current pressure to normalize environmental protection, how servitization can drive low-carbon development in MI while taking into account economic development has become an important topic at present. Thus, this study constructs an evaluation index system of factors based on a driver–pressure–state–impact–response (DPSIR) model, and uses the decision-making trial and evaluation laboratory (DEMATEL) and interpretive structural modeling (ISM) model to analyze the causal relationship and key elements among the influencing factors. The results show that from the perspective of the ecological environment, many factors affect MS, although to varying degrees. Among them, the proportion of R&D personnel, input intensity, and the proportion of clean energy are the main factors. Based on the transmission mechanism among these factors, we propose two paths to realizing the service-oriented, low-carbon development of China’s MI

Exploring the Rheological and Structural Characteristics of Novel Pectin-Salecan Gels

The hydrogels based on natural polysaccharide offers high hydrophilicity and excellent biocompatibility while exhibiting soft physical properties related to texture and tissues, making them ideal candidates for food and biomedical applications. Herein, a new gel system composed of pectin and salecan (PS) was designed and prepared, and its structural and functional characteristics were further explored by scanning electron microscopy and rheological testing. Data fitting based on Herschel–Bulkley (HB) and Power-Law models enable in-depth comparisons and elucidations of the PS gels’ flow behavior. The cyclic strain time scanning test gave an interesting maximum strain recovery rate of about 70%; meanwhile, the creep data reported an adjustable creep compliance of 0.0146 to 0.1802. The comprehensive analysis of the structure and rheological exploration of the novel pectin-salecan hydrogels demonstrated their potential advantages over pectin and broader applicability in different food or biomedical fields

Assessing the Link between Human Modification and Changes in Land Surface Temperature in Hainan, China Using Image Archives from Google Earth Engine

In many areas of the world, population growth and land development have increased demand for land and other natural resources. Coastal areas are particularly susceptible since they are conducive for marine transportation, energy production, aquaculture, marine tourism and other activities. Anthropogenic activities in the coastal areas have triggered unprecedented land use change, depletion of coastal wetlands, loss of biodiversity, and degradation of other vital ecosystem services. The changes can be particularly drastic for small coastal islands with rich biodiversity. In this study, the influence of human modification on land surface temperature (LST) for the coastal island Hainan in Southern China was investigated. We hypothesize that for this island, footprints of human activities are linked to the variation of land surface temperature, which could indicate environmental degradation. To test this hypothesis, we estimated LST changes between 2000 and 2016 and computed the spatio-temporal correlation between LST and human modification. Specifically, we classified temperature data for the four years 2000, 2006, 2012 and 2016 into 5 temperature zones based on their respective mean and standard deviation values. We then assessed the correlation between each temperature zone and a human modification index computed for the year 2016. Apart from this, we estimated mean, maximum and the standard deviation of annual temperature for each pixel in the 17 years to assess the links with human modification. The results showed that: (1) The mean LST temperature in Hainan Island increased with fluctuations from 2000 to 2016. (2) The moderate temperature zones were dominant in the island during the four years included in this study. (3) A strong positive correlation of 0.72 between human modification index and mean and maximum LST temperature indicated a potential link between human modification and mean and maximum LST temperatures over the 17 years of analysis. (4) The mean value of human modification index in the temperature zones in 2016 showed a progressive rise with 0.24 in the low temperature zone, 0.33 in the secondary moderate, 0.45 in the moderate, 0.54 in the secondary high and 0.61 in the high temperature zones. This work highlighted the potential value of using large and multi-temporal earth observation datasets from cloud platforms to assess the influence of human activities in sensitive ecosystems. The results could contribute to the development of sustainable management and coastal ecosystems conservation plans

Connecting Customers and Merchants Offline: Experimental Evidence from the Commercialization of Last-mile Stations at Alibaba

Many e-commerce platforms have established extensive networks of stations as their last-mile logistics infrastructure. This study investigates how this last-mile infrastructure may serve as an offline platform to connect customers and merchants in the physical world by leveraging walk-in traffic (organic interaction) and prompting interested customers through online intervention (induced interaction). Using free sample distribution as an example, we designed two large-scale studies in collaboration with Alibaba—an observational study across 1,032 stations and a randomized field experiment among 189,019 customers—to examine the causal effects of organic and induced interactions on customers’ subsequent online purchases from the focal brands, respectively. We found that induced interaction drives significantly more online sales than organic interaction. Under induced interaction, the online intervention effectively increases the number of free samples distributed. Nevertheless, the more significant increase in online sales for induced claimers is not simply due to more free samples distributed but because the induced customers are more interested and more likely to purchase. We identified this phenomenon as a screening mechanism that facilitates an advantageous selection of customers claiming the samples. Customers willing to pay the additional travel cost to claim samples are more likely to purchase the focal brands afterward. Finally, we developed a customized targeting framework using a generalized random forest model to enhance the effectiveness of induced interaction at the last-mile stations. Our study raises a key insight that the “foot-in-the-door” traffic in an omnichannel environment can be fundamentally different depending on whether the offline customers are driven from the online channel