Effect of oxytocin infusion on luteal blood flow and progesterone secretion in dairy cattle

The objective of this study was to investigate the effects of oxytocin infusion on corpus luteum (CL) function during early to mid-diestrus by measuring luteal size (LS) and luteal blood flow (LBF) along with plasma levels of progesterone (P4) and prostaglandin metabolites (13,14-dihydro-15-keto-prostaglandin F2α, PGFM). On day (D) 7 of the estrus cycle (D1 = ovulation), seven cows received 100 IU of oxytocin (OXY) or placebo (PL) following a Latin square design. LS and LBF increased in both groups over time and no differences were observed between the groups. PGFM did not differ either within the groups over time or between the groups at any time point. P4 of the OXY group was higher compared to that of the the PL group 360 min after the infusion (p = 0.01) and tended to be higher at the time points 450 min, 48 h, and 72 h (all p = 0.08). Results from this study support the hypothesis that OXY is not directly involved in the mechanism(s) governing blood flow of the CL and has no remarkable effects either on luteal size or P4 and PGFM plasma levels. Further investigation is needed to elucidate the role of OXY in CL blood flow during early and late luteal phases

Energy Conservation in a Livestock Building Combined with a Renewable Energy Heating System towards CO2 Emission Reduction: The Case Study of a Sheep Barn in North Greece

Cold stress in sheep is usually overlooked, even though the animals’ welfare and productivity are affected by low temperatures. The aim of this research was to find out if and to what extent the temperature inside a sheep barn could be maintained within the range of the thermoneutral zone during winter, primarily to increase feed conversion and to reduce GHG emissions. For this reason, an automation system was installed at a sheep barn in northern Greece, and heat losses from the building were calculated. The biogas potential of the sheep barn waste was examined in the laboratory via the BMP method. The results showed that the installation of an automation system together with a hypothetical biogas heating system could maintain the barn’s temperature in the range of a sheep’s thermoneutral zone during winter for the 94% of the scenarios examined if the total energy of the biogas was utilized, while heating energy that was instantly and continuously used succeeded in 48% of the investigated cases. The surplus of energy produced by biogas could potentially raise the water temperature that animals drink up to 2.9 °C. The absence of cold stress decreases the dry matter intake and the CH4 produced by ruminal fermentation. Moreover, lower GHG emissions are achieved as waste is treated through anaerobic digestion, which would likely be released into the environment if left untreated

Evidence of Schmallenberg virus circulation in ruminants in Greece

International audienceDuring March 2013, we investigated the presence and the levels of Schmallenberg virus (SBV) circulation in three dairy cow herds and three sheep flocks in Central Macedonia, Greece. In two cow herds, a high number of abortions had been observed during the winter. Six bulk-tank milk samples and 147 individual sera were screened for SBV-specific antibodies by ELISA. Positive reactions were obtained from 5 out of 6 bulk-tank milk samples, 58 out of 90 sera from the 3 cow herds, and 2 sera from 2 of the 3 sheep flocks. Twenty-two ELISA-positive sera were tested by serum neutralization test (SNT). SNT confirmed the presence of neutralizing antibodies against SBV in all samples tested, with titers ranging between 1:32 and a parts per thousand yen1:256. No neutralizing antibodies against Akabane virus (AKAV) or Shamonda virus (SHAV) were detected, indicating that neutralizing antibodies against SBV do not cross react with AKAV or SHAV in SNT. ELISA testing of bulk-tank milk samples proved to be convenient and reliable. None of the tested sera was found positive for SBV by real-time RT-PCR, indicating that the sampling was conducted past the viremia stage. This is the first report of SBV circulation in Greece