Bosea psychrotolerans sp. nov., a psychrotrophic alphaproteobacterium isolated from Lake Michigan water

Albert, Richard A., McGuine, Molly, Pavlons, Shawn C., Roecker, Jon, Bruess, Jennifer, Mossman, Shane, Sun, Sona, King, Mike, Hong, Sunhee, Farrance, Christine E., Danner, Joseph, Joung, Yochan, Shapiro, Nicole, Whitman, William B., Busse, Hans-Jürgen (2019): Bosea psychrotolerans sp. nov., a psychrotrophic alphaproteobacterium isolated from Lake Michigan water. International Journal of Systematic and Evolutionary Microbiology 69 (5): 1376-1383, DOI: 10.1099/ijsem.0.003319, URL: http://dx.doi.org/10.1099/ijsem.0.00331

Bosea psychrotolerans Albert & McGuine & Pavlons & Roecker & Bruess & Mossman & Sun & King & Hong & Farrance & Danner & Joung & Shapiro & Whitman & Busse 2019, SP. NOV.

DESCRIPTION OF BOSEA PSYCHROTOLERANS SP. NOV. Bosea psychrotolerans (psy.chro.to′ le.rans. Gr. adj. psychros, cold; L. pres. part. tolerans, tolerating; N.L. part. adj. psychrotolerans, cold-tolerating). Cells are short rods, 2–3 µm long and 0.4–0.5 µm in diameter that occur singly or in pairs and form rosettes. Gramstain-negative. Isolated colonies on PCA after 48 h incubation at 25 Ǫ C range from 0.5 to 1 mm in diameter with an entire edge, flat and circular colony shape. Colonies have a tan colour and produce copious amount of extracellular material. Produces water soluble brown pigment when grown on PCA. The temperature growth range is 5–35 Ǫ C, the pH growth range is 5.5 to 8.0, while the NaCl tolerance for growth was 0.0 to 1.4 % (w/v). Aerobic, motile, reduces nitrate, catalase-negative and oxidase-negative. Capsules not produced. Positive for use of D- glucose, D- fructose, cellobiose, D- xylose, D- galactose, D- arabinose, D- ribose, sodium gluconate, Tween 20, Tween 80, sodium citrate, leucine and valine as growth substrates, but not lactose, maltose, D- mannitol, D- rhamnose, raffinose, D- glycerol, sucrose, trehalose, D- inositol, sodium acetate, sodium propionate, L- lysine, L- histidine and L- threonine. Casein, starch and gelatin are not hydrolysed. The polyamine pattern contains predominantly sym -homospermidine and ubiquinone Q-10 is the major compound in the quinone system. The polar lipid profile is composed of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine as major lipids, with moderate to minor amounts of phosphatidylmonomethylethanolamine, two unidentified aminolipids (AL1, AL2), one unidentified glycolipid (GL1) and two unidentified polar lipids (L1, L3) lacking a functional group. The organism was isolated from a Lake Michigan potable water sample in south-eastern Wisconsin. Type strain 1131 T has been deposited as LMG 30034 and as NRRL B- 65405. The GenBank accession number for the 16S rRNA gene sequence of strain 1131 T is MG687375. The GenBank accession number for the entire genome of strain 1131 T is PQFZ00000000. The DNA G+C content of strain 1131 T is 66.65 mol%.Published as part of Albert, Richard A., McGuine, Molly, Pavlons, Shawn C., Roecker, Jon, Bruess, Jennifer, Mossman, Shane, Sun, Sona, King, Mike, Hong, Sunhee, Farrance, Christine E., Danner, Joseph, Joung, Yochan, Shapiro, Nicole, Whitman, William B. & Busse, Hans-Jürgen, 2019, Bosea psychrotolerans sp. nov., a psychrotrophic alphaproteobacterium isolated from Lake Michigan water, pp. 1376-1383 in International Journal of Systematic and Evolutionary Microbiology 69 (5) on page 1382, DOI: 10.1099/ijsem.0.003319, http://zenodo.org/record/622385

A Plant-Produced Pfs230 Vaccine Candidate Blocks Transmission of Plasmodium falciparum▿†

Plasmodium falciparum is transmitted to a new host after completing its sexual cycle within a mosquito. Developing vaccines against the parasite sexual stages is a critical component in the fight against malaria. We are targeting multiple proteins of P. falciparum which are found only on the surfaces of the sexual forms of the parasite and where antibodies against these proteins have been shown to block the progression of the parasite's life cycle in the mosquito and thus block transmission to the next human host. We have successfully produced a region of the Pfs230 antigen in our plant-based transient-expression system and evaluated this vaccine candidate in an animal model. This plant-produced protein, 230CMB, is expressed at approximately 800 mg/kg in fresh whole leaf tissue and is 100% soluble. Administration of 230CMB with >90% purity induces strong immune responses in rabbits with high titers of transmission-blocking antibodies, resulting in a greater than 99% reduction in oocyst counts in the presence of complement, as determined by a standard membrane feeding assay. Our data provide a clear perspective on the clinical development of a Pfs230-based transmission-blocking malaria vaccine