A pyrene-armed hexahomotrioxacalix[3]arene as a multi-sensor via synergistic and demetallation effects

A new pyrene-armed hexahomotrioxacalix[3]arene L has been synthesized, which exhibits a pronounced fluorescence enhancement response toward Cu²⁺ ions via a Zn²⁺ or Cd²⁺ triggered synergistic effect. Additionally, the L·Cu²⁺+ complex can subsequently serve as a sensor for F⁻ via anion-induced demetallation. The fluorescence responses by the input of Cu²⁺, Zn²⁺/Cd²⁺ and F⁻ can be constructed as a combinational logic gate which mimics a set of molecular traffic signals

Synthesis and evaluation of a novel fluorescent sensor based on hexahomotrioxacalix[3]arene for Zn²+ and Cd²+

A novel type of selective and sensitive fluorescent sensor having triazole rings as the binding sites on the lower rim of a hexahomotrioxacalix[3]arene scaffold in a cone conformation is reported. This sensor has desirable properties for practical applications, including selectivity for detecting Zn²⁺ and Cd²⁺ in the presence of excess competing metal ions at low ion concentration or as a fluorescence enhancement type chemosensor due to the cavity of calixarene changing from a ‘flattened-cone’ to a more-upright form and inhibition of PET. In contrast, the results suggested that receptor 1 is highly sensitive and selective for Cu²⁺ and Fe³⁺ as a fluorescence quenching type chemosensor due to the photoinduced electron transfer (PET) or heavy atom effect

Hepatitis B virus induces G1 phase arrest by regulating cell cycle genes in HepG2.2.15 cells

<p>Abstract</p> <p>Background</p> <p>To investigate the effect of HBV on the proliferative ability of host cells and explore the potential mechanism.</p> <p>Methods</p> <p>MTT, colony formation assay and tumourigenicity in nude mice were performed to investigate the effect of HBV on the proliferative capability of host cells. In order to explore the potential mechanism, cell cycle and apoptosis were analysed. The cell cycle genes controlling the G1/S phase transition were detected by immunohistochemistry, westernblot and RT-PCR.</p> <p>Results</p> <p>HepG2.2.15 cells showed decreased proliferation ability compared to HepG2 cells. G1 phase arrest was the main cause but was not associated with apoptosis. p53, p21 and total retinoblastoma (Rb) were determined to be up-regulated, whereas cyclinE was down-regulated at both the protein and mRNA levels in HepG2.2.15 cells. The phosphorylated Rb in HepG2.2.15 cells was decreased.</p> <p>Conclusions</p> <p>Our results suggested that HBV inhibited the capability of proliferation of HepG2.2.15 cells by regulating cell cycle genes expression and inducing G1 arrest.</p

Hepatitis B virus induces G1 phase arrest by regulating cell cycle genes in HepG2.2.15 cells

<p>Abstract</p> <p>Background</p> <p>To investigate the effect of HBV on the proliferative ability of host cells and explore the potential mechanism.</p> <p>Methods</p> <p>MTT, colony formation assay and tumourigenicity in nude mice were performed to investigate the effect of HBV on the proliferative capability of host cells. In order to explore the potential mechanism, cell cycle and apoptosis were analysed. The cell cycle genes controlling the G1/S phase transition were detected by immunohistochemistry, westernblot and RT-PCR.</p> <p>Results</p> <p>HepG2.2.15 cells showed decreased proliferation ability compared to HepG2 cells. G1 phase arrest was the main cause but was not associated with apoptosis. p53, p21 and total retinoblastoma (Rb) were determined to be up-regulated, whereas cyclinE was down-regulated at both the protein and mRNA levels in HepG2.2.15 cells. The phosphorylated Rb in HepG2.2.15 cells was decreased.</p> <p>Conclusions</p> <p>Our results suggested that HBV inhibited the capability of proliferation of HepG2.2.15 cells by regulating cell cycle genes expression and inducing G1 arrest.</p

Formation of Lower Mass-gap Black Hole--Neutron Star Binary Mergers through Super-Eddington Stable Mass Transfer

Super-Eddington accretion of neutron stars (NSs) has been suggested both observationally and theoretically. In this paper, we propose that NSs in close-orbit binary systems with companions of helium (He) stars, most of which systems form after the common-envelope phase, could experience super-Eddington stable Case BB/BC mass transfer (MT), and can sometimes occur accretion-induced collapses (AICs) to form lower mass-gap black holes (mgBHs). Our detailed binary evolution simulations reveal that AIC events tend to happen if the primaries NS have an initial mass $\gtrsim1.7\,M_\odot$ with an accretion rate of $\gtrsim300$ times the Eddington limit. These mgBHs would have a mass nearly equal to or slightly higher than the NS maximum mass. The remnant mgBH--NS binaries after the core collapses of He stars are potential progenitors of gravitational-wave (GW) source. Multimessenger observation between GW and kilonova signals from a population of high-mass binary NS and mgBH--NS mergers formed through super-Eddington stable MT are helpful in constraining the maximum mass and equation of state of NSs. S230529ay, a mgBH--NS merger candidate recently detected in the fourth observing run of the LIGO-Virgo-KAGRA Collaboration, could possibly originate from this formation scenario.Comment: Submitted to MNRAS on September 29th, 10 pages, 5 figures, comments are welcom

Non-coding RNAs participate in the regulatory network of CLDN4 via ceRNA mediated miRNA evasion

AbstractThousands of genes have been well demonstrated to play important roles in cancer progression. As genes do not function in isolation, they can be grouped into “networks” based on their interactions. In this study, we discover a network regulating Claudin-4 in gastric cancer. We observe that Claudin-4 is up-regulated in gastric cancer and is associated with poor prognosis. Claudin-4 reinforce proliferation, invasion, and EMT in AGS, HGC-27, and SGC-7901 cells, which could be reversed by miR-596 and miR-3620-3p. In addition, lncRNA-KRTAP5-AS1 and lncRNA-TUBB2A could act as competing endogenous RNAs to affect the function of Claudin-4. Our results suggest that non-coding RNAs play important roles in the regulatory network of Claudin-4. As such, non-coding RNAs should be considered as potential biomarkers and therapeutic targets against gastric cancer.</jats:p

Prognostic significance of peripheral and tumor-infiltrating lymphocytes in newly diagnosed stage III/IV non-small-cell lung cancer

Background and aimLymphocytes are effector cells that fight cancer by killing tumor cells. Here, we aim to explore the prognostic significance of both peripheral and tumor-infiltrating lymphocytes (TILs) in newly diagnosed stage III/IV non-small-cell lung cancer (NSCLC).Materials and methodsIn total, 105 cases of newly diagnosed stage III/IV NSCLC from July 2017 to October 2022 at the Tianjin Beichen Hospital were retrospectively investigated. Peripheral blood samples at the time of diagnosis and tumor tissue slices from these patients were collected. General peripheral blood cell composition and TILs were measured and analyzed via an automatic blood analyzer and immunofluorescence staining analysis. The overall survival (OS) time of all patients was also obtained and analyzed.ResultsThe median overall survival (mOS) of all patients is 12 months. The 1-, 2-, and 3-year overall survival rates were 60.5, 28.4, and 18.6%, respectively. Peripheral lymphocyte and neutrophil percentages, serum C-reactive protein (CRP) expression, tumor size, and tumor pathology are the prognostic factors of OS for newly diagnosed stage III/IV NSCLC patients. Moreover, patients with high tumor CD4+ and CD8+ T cell infiltration survived significantly longer compared to patients with low tumor CD4+ and CD8+ T cell infiltration (p &lt; 0.0001 and p = 0.011, respectively). Compared to low tumor CD33+ cell infiltration, high tumor CD33+ cell infiltration was associated with worse OS (p = 0.018). High tumor CD8+ T cell infiltration was associated with lower peripheral lymphocyte number, lower serum CRP expression, smaller tumor size, and better tumor pathology (p = 0.012, p = 0.040, p = 0.012, and p = 0.029, respectively).ConclusionIncreased numbers of peripheral lymphocytes, CD33+ cells, CD4+ TILs, and CD8+ TILs were significantly associated with OS in newly diagnosed stage III/IV NSCLC patients, which were positively associated with several basic clinical factors

Vortex Dynamics in Rotating Rayleigh-B\'enard Convection

We investigate the spatial distribution and dynamics of the vortices in rotating Rayleigh-B\'enard convection in a reduced Rayleigh-number range $1.3{\le}Ra/Ra_{c}{\le}166$. Under slow rotations ($Ra{\gtrsim}10Ra_{c}$), the vortices are randomly distributed. The size-distribution of the Voronoi cells of the vortex centers is well described by the standard $\Gamma$ distribution. In this flow regime the vortices exhibit Brownian-type horizontal motion. The probability density functions of the vortex displacements are, however, non-Gaussian at short time scales. At modest rotating rates ($4Ra_{c}{\le}Ra{\lesssim}10Ra_{c}$) the centrifugal force leads to radial vortex motions, i.e., warm cyclones (cold anticyclones) moving towards (outward from) the rotation axis. The mean-square-displacements of the vortices increase faster than linearly at large time. This super-diffusive behavior can be satisfactorily explained by a Langevin model incorporating the centrifugal force. In the rapidly rotating regime ($1.6Ra_{c}{\le}Ra{\le}4Ra_{c}$) the vortices are densely distributed, with the size-distribution of their Voronoi cells differing significantly from the standard $\Gamma$ distribution. The hydrodynamic interaction of neighboring vortices results in formation of vortex clusters. Inside clusters the correlation of the vortex velocity fluctuations is scale free, with the correlation length being approximately $30\%$ of the cluster length. We examine the influence of cluster forming on the dynamics of individual vortex. Within clusters, cyclones exhibit inverse-centrifugal motion as they submit to the motion of strong anticyclones, while the velocity for outward motion of the anticyclones is increased. Our analysis show that the mobility of isolated vortices, scaled by their vorticity strength, is a simple power function of the Froude number

Demonstration of Adiabatic Variational Quantum Computing with a Superconducting Quantum Coprocessor

Adiabatic quantum computing enables the preparation of many-body ground states. This is key for applications in chemistry, materials science, and beyond. Realisation poses major experimental challenges: Direct analog implementation requires complex Hamiltonian engineering, while the digitised version needs deep quantum gate circuits. To bypass these obstacles, we suggest an adiabatic variational hybrid algorithm, which employs short quantum circuits and provides a systematic quantum adiabatic optimisation of the circuit parameters. The quantum adiabatic theorem promises not only the ground state but also that the excited eigenstates can be found. We report the first experimental demonstration that many-body eigenstates can be efficiently prepared by an adiabatic variational algorithm assisted with a multi-qubit superconducting coprocessor. We track the real-time evolution of the ground and exited states of transverse-field Ising spins with a fidelity up that can reach about 99%.Comment: 12 pages, 4 figure