3 research outputs found
The role of Xpert MTB/RIF in diagnosing pulmonary tuberculosis in post-mortem tissues
The extent to which the Xpert MTB/RIF (Gene Xpert) contributes
to tuberculosis (TB) diagnosis in samples other than sputum and
cerebrospinal fluid remains uncertain. We aimed to assess the
role of Xpert MTB/RIF for detecting M. tuberculosis in
post-mortem tissues. We conducted a study among 30 complete
diagnostic autopsies (CDA) performed at the Maputo Central
Hospital (Mozambique). Lung tissues were screened for TB in all
cases. In addition other tissues were tested when compatible
lesions were identified in the histological exam. We used
in-house real time PCR and LAMP assays to confirm the presence
of M. tuberculosis DNA. The diagnosis of tuberculosis at death
was established based on microbiological and histopathological
results. Eight out of 30 cases (26.7%) were diagnosed of
tuberculosis. Xpert had a sensitivity to detect TB in lung
tissue of 87.5% (95% CI 47.3-99.7) and a specificity of 95.7%
(95% CI: 78.1-99.9). In-house DNA amplification methods and
Xpert showed 93.6% concordance for lung tissue and 100%
concordance for brain and liver tissues. The final cause of
death was attributable to tuberculosis in four cases. Xpert
MTB/RIF may represent a valuable, easy-to perform technique for
post-mortem TB diagnosis
The role of Xpert MTB/RIF in diagnosing pulmonary tuberculosis in post-mortem tissues
The extent to which the Xpert MTB/RIF (Gene Xpert) contributes
to tuberculosis (TB) diagnosis in samples other than sputum and
cerebrospinal fluid remains uncertain. We aimed to assess the
role of Xpert MTB/RIF for detecting M. tuberculosis in
post-mortem tissues. We conducted a study among 30 complete
diagnostic autopsies (CDA) performed at the Maputo Central
Hospital (Mozambique). Lung tissues were screened for TB in all
cases. In addition other tissues were tested when compatible
lesions were identified in the histological exam. We used
in-house real time PCR and LAMP assays to confirm the presence
of M. tuberculosis DNA. The diagnosis of tuberculosis at death
was established based on microbiological and histopathological
results. Eight out of 30 cases (26.7%) were diagnosed of
tuberculosis. Xpert had a sensitivity to detect TB in lung
tissue of 87.5% (95% CI 47.3-99.7) and a specificity of 95.7%
(95% CI: 78.1-99.9). In-house DNA amplification methods and
Xpert showed 93.6% concordance for lung tissue and 100%
concordance for brain and liver tissues. The final cause of
death was attributable to tuberculosis in four cases. Xpert
MTB/RIF may represent a valuable, easy-to perform technique for
post-mortem TB diagnosis
The role of Xpert MTB/RIF in diagnosing pulmonary tuberculosis in post-mortem tissues
The extent to which the Xpert MTB/RIF (Gene Xpert) contributes
to tuberculosis (TB) diagnosis in samples other than sputum and
cerebrospinal fluid remains uncertain. We aimed to assess the
role of Xpert MTB/RIF for detecting M. tuberculosis in
post-mortem tissues. We conducted a study among 30 complete
diagnostic autopsies (CDA) performed at the Maputo Central
Hospital (Mozambique). Lung tissues were screened for TB in all
cases. In addition other tissues were tested when compatible
lesions were identified in the histological exam. We used
in-house real time PCR and LAMP assays to confirm the presence
of M. tuberculosis DNA. The diagnosis of tuberculosis at death
was established based on microbiological and histopathological
results. Eight out of 30 cases (26.7%) were diagnosed of
tuberculosis. Xpert had a sensitivity to detect TB in lung
tissue of 87.5% (95% CI 47.3-99.7) and a specificity of 95.7%
(95% CI: 78.1-99.9). In-house DNA amplification methods and
Xpert showed 93.6% concordance for lung tissue and 100%
concordance for brain and liver tissues. The final cause of
death was attributable to tuberculosis in four cases. Xpert
MTB/RIF may represent a valuable, easy-to perform technique for
post-mortem TB diagnosis