Does prior experience really matter for disaster preparedness?

Climate change and transition toward urbanization had resulted in more frequent and severe disasters in the last decade. While urbanization is potential driver of change and economic growth, climate change had exacerbated resource scarcity and exposed local communities to greater and more frequent disasters. The local communities in Malaysia are of no exception. The most common types of disasters in Malaysia are landslide, flash flood and haze. This study explores the extent to which prior experience affects the level of preparedness among Malaysian communities before occurrence of natural disasters. Although previous studies have proven prior experience aided better handling of preparedness, yet some researchers have not found significant correlation between prior experience and preparedness. Having inconclusive results and with the rise of unexpected disaster events, has lead this present study to investigate the link in the Malaysian context. Questionnaire surveys were distributed to more than 1,000 respondents and face-to-face interviews were conducted with 12 local communities from Klang Valley, Malaysia. The results indicated that prior experience does contribute towards the level of disaster preparedness among the local communities in Malaysia. Hence, the findings of this present study may reduce the impact of disasters and thus could help local communities to effectively cope with the events of disasters

Multiple Events of Allopolyploidy in the Evolution of the Racemose Lineages in Prunus (Rosaceae) Based on Integrated Evidence from Nuclear and Plastid Data.

Prunus is an economically important genus well-known for cherries, plums, almonds, and peaches. The genus can be divided into three major groups based on inflorescence structure and ploidy levels: (1) the diploid solitary-flower group (subg. Prunus, Amygdalus and Emplectocladus); (2) the diploid corymbose group (subg. Cerasus); and (3) the polyploid racemose group (subg. Padus, subg. Laurocerasus, and the Maddenia group). The plastid phylogeny suggests three major clades within Prunus: Prunus-Amygdalus-Emplectocladus, Cerasus, and Laurocerasus-Padus-Maddenia, while nuclear ITS trees resolve Laurocerasus-Padus-Maddenia as a paraphyletic group. In this study, we employed sequences of the nuclear loci At103, ITS and s6pdh to explore the origins and evolution of the racemose group. Two copies of the At103 gene were identified in Prunus. One copy is found in Prunus species with solitary and corymbose inflorescences as well as those with racemose inflorescences, while the second copy (II) is present only in taxa with racemose inflorescences. The copy I sequences suggest that all racemose species form a paraphyletic group composed of four clades, each of which is definable by morphology and geography. The tree from the combined At103 and ITS sequences and the tree based on the single gene s6pdh had similar general topologies to the tree based on the copy I sequences of At103, with the combined At103-ITS tree showing stronger support in most clades. The nuclear At103, ITS and s6pdh data in conjunction with the plastid data are consistent with the hypothesis that multiple independent allopolyploidy events contributed to the origins of the racemose group. A widespread species or lineage may have served as the maternal parent for multiple hybridizations involving several paternal lineages. This hypothesis of the complex evolutionary history of the racemose group in Prunus reflects a major step forward in our understanding of diversification of the genus and has important implications for the interpretation of its phylogeny, evolution, and classification

Spatiotemporal dipole source localization of face processing ERPs in adolescents: a preliminary study

<p>Abstract</p> <p>Background</p> <p>Despite extensive investigation of the neural systems for face perception and emotion recognition in adults and young children in the past, the precise temporal activation of brain sources specific to the processing of emotional facial expressions in older children and adolescents is not well known. This preliminary study aims to trace the spatiotemporal dynamics of facial emotion processing during adolescence and provide a basis for future developmental studies and comparisons with patient populations that have social-emotional deficits such as autism.</p> <p>Methods</p> <p>We presented pictures showing happy, angry, fearful, or neutral facial expressions to healthy adolescents (aged 10–16 years) and recorded 128-channel event-related potentials (ERPs) while they performed an emotion discrimination task. ERP components were analyzed for effects of age and emotion on amplitude and latency. The underlying cortical sources of scalp ERP activity were modeled as multiple equivalent current dipoles using Brain Electrical Source Analysis (BESA).</p> <p>Results</p> <p>Initial global/holistic processing of faces (P1) took place in the visual association cortex (lingual gyrus) around 120 ms post-stimulus. Next, structural encoding of facial features (N170) occurred between 160–200 ms in the inferior temporal/fusiform region, and perhaps early emotion processing (Vertex Positive Potential or VPP) in the amygdala and orbitofrontal cortex. Finally, cognitive analysis of facial expressions (P2) in the prefrontal cortex and emotional reactions in somatosensory areas were observed from about 230 ms onwards. The temporal sequence of cortical source activation in response to facial emotion processing was occipital, prefrontal, fusiform, parietal for young adolescents and occipital, limbic, inferior temporal, and prefrontal for older adolescents.</p> <p>Conclusion</p> <p>This is a first report of high-density ERP dipole source analysis in healthy adolescents which traces the sequence of neural activity within the first 500 ms of categorizing emotion from faces. Our spatio-temporal brain source models showed the presence of adult-like cortical networks for face processing in adolescents, whose functional specificity to different emotions appear to be not yet fully mature. Age-related differences in brain activation patterns illustrate the continued development and maturation of distinct neural systems for processing facial expressions during adolescence and possible changes in emotion perception, experience, and reaction with age.</p

Removal of nutrients and heavy metals from domestic and industry using botryococcus sp.

Microphytes or microalgae are the most basic food source of many types of organisms on earth and blooms during the presence of dissolved inorganic phosphorus. Wastewater is a body of water that is dangerous to organic life forms when consumed or used. It contains many pollutants that can cause health problems and also affect the ecosystem of an environment. This study aims to improve the water quality of wastewaters using phycoremediation process. The objectives of this study are to determine the growth of Botryococcus sp. in different types of wastewater in terms of resistance and survival of Botryococcus sp. in phycoremediation performance, to measure the environmental factor effecting the growth of Botryococcus sp. of phycoremediation process, to optimize the physiochemical and heavy metal removal in different types of wastewaters and to evaluate the effectiveness of Botryococcus sp. to remove the pollutants in wastewaters. Phycoremediation or bioremediation process is using macroalgae or microalgae for removing pollutants, nutrients, xenobiotics and heavy metals from wastewater. This research was done by collecting microalgae sample, isolating and culturing the required Botryococcus sp. Growth optimization and followed by phycoremediation process is done to remove unwanted elements from wastewaters. The optimum growth rate of algae is achieved when salinity is at 0M, temperature at 330C, photoperiod at 12:12 and light intensity of 18000 Lux. Result shows that the highest nitrate removal percentage occurs in semiconductor (100%), followed by palm oil mill effluent (97.29%), textile wastewater (98.04%) and domestic wastewater (85.43%). Total Phosphorus removal indicates the highest percentage for domestic wastewater (100%), palm oil effluent (99.2%), textile wastewater (98.44%) and semiconductor (50.39%). From this research, it is found that the best overall removal of physiochemical and heavy metal content occurs in palm oil mill effluent followed by domestic wastewater, semiconductor wastewater and textile wastewater

Application of an evolutionary algorithm-based ensemble model to job-shop scheduling

In this paper, a novel evolutionary algorithm is applied to tackle job-shop scheduling tasks in manufacturing environments. Specifically, a modified micro genetic algorithm (MmGA) is used as the building block to formulate an ensemble model to undertake multi-objective optimisation problems in job-shop scheduling. The MmGA ensemble is able to approximate the optimal solution under the Pareto optimality principle. To evaluate the effectiveness of the MmGA ensemble, a case study based on real requirements is conducted. The results positively indicate the effectiveness of the MmGA ensemble in undertaking job-shop scheduling problems

Malaysian Healthy Diet Online Survey (MHDOS): Study rationale and methodology

Introduction: Access to accurate and timely dietary information is of paramount importance in evaluating and developing well-targeted public health nutrition interventions. However, nationwide nutrition surveys are conducted infrequently because they are very costly to design, conduct and analyse. Dietary assessment tools, which are quick and cost- effective, are needed for population research and regular monitoring of Malaysians’ dietary habits. This paper describes the rationale and methodology of the Malaysian Healthy Diet Online Survey (MHDOS) project, which aims to bridge this knowledge gap on dietary intake of Malaysian adults. The main objective of the two-year project is to develop MHDOS as a valid tool to measure compliance with the Malaysian Dietary Guidelines 2020. Methods: The MHDOS project has three study phases, namely (i) adaptation of an online survey and established diet quality scoring system for Malaysia, (ii) usability, validity and reliability testing of the online survey; and (iii) online survey administration in a nationwide study. The survey will be administered to approximately 10,000 Malaysian adults aged 18-59 years. Discussion: MHDOS consists of 38 questions that measures the quantity, quality and variety of foods consumed. Individuals will receive a diet quality score that reflects their overall compliance with the Malaysian Dietary Guidelines and feedback on how to improve their scores. The findings of the online survey, which serve to complement information between larger surveys, will be useful to measure compliance of Malaysians to national dietary guidelines and inform public health interventions

Does the Axillary Lymph Node Ratio Have Any Added Prognostic Value over pN Staging for South East Asian Breast Cancer Patients?

10.1371/journal.pone.0045809PLoS ONE79

In vitro growth of human keratinocytes and oral cancer cells into microtissues: an aerosol-based microencapsulation technique

Cells encapsulation is a micro-technology widely applied in cell and tissue research, tissue transplantation, and regenerative medicine. In this paper, we proposed a growth of microtissue model for the human keratinocytes (HaCaT) cell line and an oral squamous cell carcinoma (OSCC) cell line (ORL-48) based on a simple aerosol microencapsulation technique. At an extrusion rate of 20 µL/min and air flow rate of 0.3 L/min programmed in the aerosol system, HaCaT and ORL-48 cells in alginate microcapsules were encapsulated in microcapsules with a diameter ranging from 200 to 300 µm. Both cell lines were successfully grown into microtissues in the microcapsules of alginate within 16 days of culture. The microtissues were characterized by using a live/dead cell viability assay, field emission-scanning electron microscopy (FE-SEM), fluorescence staining, and cell re-plating experiments. The microtissues of both cell types were viable after being extracted from the alginate membrane using alginate lyase. However, the microtissues of HaCaT and ORL-48 demonstrated differences in both nucleus size and morphology. The microtissues with re-associated cells in spheroids are potentially useful as a cell model for pharmacological studies

In Vitro Growth of Human Keratinocytes and Oral Cancer Cells into Microtissues: An Aerosol-Based Microencapsulation Technique

Cells encapsulation is a micro-technology widely applied in cell and tissue research, tissue transplantation, and regenerative medicine. In this paper, we proposed a growth of microtissue model for the human keratinocytes (HaCaT) cell line and an oral squamous cell carcinoma (OSCC) cell line (ORL-48) based on a simple aerosol microencapsulation technique. At an extrusion rate of 20 �L/min and air flow rate of 0.3 L/min programmed in the aerosol system, HaCaT and ORL-48 cells in alginate microcapsules were encapsulated in microcapsules with a diameter ranging from 200 to 300 �m. Both cell lines were successfully grown into microtissues in the microcapsules of alginate within 16 days of culture. The microtissues were characterized by using a live/dead cell viability assay, field emission-scanning electron microscopy (FE-SEM), fluorescence staining, and cell re-plating experiments. The microtissues of both cell types were viable after being extracted from the alginate membrane using alginate lyase. However, the microtissues of HaCaT and ORL-48 demonstrated differences in both nucleus size and morphology. The microtissues with re-associated cells in spheroids are potentially useful as a cell model for pharmacological studies

Union commitment, perceived behavioral control and union participation

Research Paper Series (National University of Singapore. Faculty of Business Administration); 2003-0121-2