Characterization of two isolates of Trypanosoma cruzi obtained from the patient Berenice, the first human case of Chagas’ disease described by Carlos Chagas in 1909

Submitted by sandra infurna ([email protected]) on 2016-06-29T16:46:02Z No. of bitstreams: 1 carlos22_morel_etal_IOC_1996.pdf: 246408 bytes, checksum: 8cfbf156c135a4c79323e614791b9036 (MD5)Approved for entry into archive by sandra infurna ([email protected]) on 2016-06-29T16:55:46Z (GMT) No. of bitstreams: 1 carlos22_morel_etal_IOC_1996.pdf: 246408 bytes, checksum: 8cfbf156c135a4c79323e614791b9036 (MD5)Made available in DSpace on 2016-06-29T16:55:46Z (GMT). No. of bitstreams: 1 carlos22_morel_etal_IOC_1996.pdf: 246408 bytes, checksum: 8cfbf156c135a4c79323e614791b9036 (MD5) Previous issue date: 1996Submitted by Angelo Silva ([email protected]) on 2016-07-07T11:16:54Z No. of bitstreams: 3 carlos22_morel_etal_IOC_1996.pdf.txt: 17230 bytes, checksum: 8f5b2bfe8f41a8edc21225d08ba10da6 (MD5) carlos22_morel_etal_IOC_1996.pdf: 246408 bytes, checksum: 8cfbf156c135a4c79323e614791b9036 (MD5) license.txt: 2991 bytes, checksum: 5a560609d32a3863062d77ff32785d58 (MD5)Approved for entry into archive by sandra infurna ([email protected]) on 2016-07-07T12:16:21Z (GMT) No. of bitstreams: 3 license.txt: 2991 bytes, checksum: 5a560609d32a3863062d77ff32785d58 (MD5) carlos22_morel_etal_IOC_1996.pdf: 246408 bytes, checksum: 8cfbf156c135a4c79323e614791b9036 (MD5) carlos22_morel_etal_IOC_1996.pdf.txt: 17230 bytes, checksum: 8f5b2bfe8f41a8edc21225d08ba10da6 (MD5)Made available in DSpace on 2016-07-07T12:16:21Z (GMT). No. of bitstreams: 3 license.txt: 2991 bytes, checksum: 5a560609d32a3863062d77ff32785d58 (MD5) carlos22_morel_etal_IOC_1996.pdf: 246408 bytes, checksum: 8cfbf156c135a4c79323e614791b9036 (MD5) carlos22_morel_etal_IOC_1996.pdf.txt: 17230 bytes, checksum: 8f5b2bfe8f41a8edc21225d08ba10da6 (MD5) Previous issue date: 1996Universidade Federal de Ouro Preto. Escola de Farmácia. Departamento de Análises Clínicas. Ouro Preto, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Parasitologia Celular e Molecular. Belo Horizonte, MG, Brasil.Two isolates of Trypanosoma cruzi were obtained from the patient Berenice, the first human case of Chagas’ disease (Chagas 1909), when she was 55 and 71 years old, respectively. The isolates were characterized on the basis of their epimastigote-trypomastigote differentiation in liquid media and of the electrophoretic pattern of EcoR1 digestion products of kinetoplast DNA (kDNA) minicircles (schizodeme) and isoenzyme patterns (zymodeme). Clear differences were found between the isolates, suggesting the occurrence of a heterogeneous population of T. cruzi in the infection of this patient

Toxoplasmosis and mental retardation: report of a case-control study

A case-control study evaluating the association between mental retardation and toxoplasmosis was conducted among 845 school children in Belo Horizonte, MG, Brazil. Cases (450) were mentally retarded children attending a public school for special education. Controls (395) were children from the regular public school system. Clinical and anthropometric examinations and interviews were carried out to determine risk factors for toxoplasmosis and mental retardation. Diagnosis of Toxoplasma gondii infection was based upon an indirect immunofluorescent test (IFA); 55% of cases and 29% of controls were positive. The Relative Odds of mental retardation in children with positive serology was 3.0 (95% CI 2.2-4.0). Maternal exposure to cats and contact with soil were associated with an increased risk of mental retardation. Retinochoroiditis was fourfold more prevalent among cases than controls and was only diagnosed in T. gondii IFA positive participants. Congenital toxoplasmosis, in its subclinical form, appears to be an important component in the etiology of mental retardation, especially in high risk (lower socio-economic) groups. The population attributable risk was estimated as 6.0 - 9.0%, suggesting the amount of mental retardation associated with this infection

Epidemiologia da infecção pelo Schistosoma mansoni em área de baixa endemicidade no Brasil: características clínicas e nutricionais

Um estudo epidemiológico seccional, tipo caso-controle, planejado para avaliar o papel desempenhado pelo estado nutricional do paciente na associação entre a intensidade da infecção pelo Schistosoma mansoni e as manifestações clínicas da esquistossomose, foi conduzido em uma área do Estado de Minas Gerais que apresentava uma baixa freqüência desta parasitose e uma baixa morbidade devido a esta infecção. Casos (256) foram definidos como participantes que apresentavam exame de fezes positivo para S. mansoni, com uma média geométrica de 90 ovos/grama de fezes. Controles (256) foram definidos como uma amostra aleatória dos pacientes com exames de fezes negativo, pareados aos casos por idade, sexo e tempo de residência na área. Os sinais e sintomas clínicos assinalados como associados à infecção pelo S. mansoni, comparando-se casos e controles, foram a presença de sangue nas fezes e de fígado palpável. Foi detectada uma tendência linear nas odds relativas destes sinais e sintomas e níveis crescentes de infecção. Ajustando pelo numera de ovos de S. mansoni excretados, a existência de uma interação entre grupo étnico (brancos) e fígado palpável foi sugerida. Não foram encontradas diferenças significativas no estado nutricional dos casos e dos controles.A cross-sectional case-control study designed to evaluate the role of malnutrition in the association between the intensity of Schistosoma mansoni infection and clinical schistosomiasis, was conducted in an area with both low frequency of infection and low morbidity of schistosomiasis in Brazil. Cases (256) were patients with a positive stool examination for S. mansoni; their geometrical mean number of eggs/gram of feces was 90. Controls (256) were a random sample of the negative participants paired to the cases by age, sex and length of residence in the area. The clinical signs and symptoms found to be associated with S. mansoni infection, comparing cases and controls, were blood in stools and presence of a palpable liver. A linear trend in the relative odds of these signs and symptoms with increasing levels of infection was detected. Adjusting by the level of egg excretion, the existence of an interaction between palpable liver and ethnic group (white) was suggested. No differences in the nutritional status of infected and non-infected participants were found