RNA aptamers generated against oligomeric Abeta40 recognize common amyloid aptatopes with low specificity but high sensitivity.

Aptamers are useful molecular recognition tools in research, diagnostics, and therapy. Despite promising results in other fields, aptamer use has remained scarce in amyloid research, including Alzheimer's disease (AD). AD is a progressive neurodegenerative disease believed to be caused by neurotoxic amyloid beta-protein (Abeta) oligomers. Abeta oligomers therefore are an attractive target for development of diagnostic and therapeutic reagents. We used covalently-stabilized oligomers of the 40-residue form of Abeta (Abeta40) for aptamer selection. Despite gradually increasing the stringency of selection conditions, the selected aptamers did not recognize Abeta40 oligomers but reacted with fibrils of Abeta40, Abeta42, and several other amyloidogenic proteins. Aptamer reactivity with amyloid fibrils showed some degree of protein-sequence dependency. Significant fibril binding also was found for the naïve library and could not be eliminated by counter-selection using Abeta40 fibrils, suggesting that aptamer binding to amyloid fibrils was RNA-sequence-independent. Aptamer binding depended on fibrillogenesis and showed a lag phase. Interestingly, aptamers detected fibril formation with > or =15-fold higher sensitivity than thioflavin T (ThT), revealing substantial beta-sheet and fibril formation undetected by ThT. The data suggest that under physiologic conditions, aptamers for oligomeric forms of amyloidogenic proteins cannot be selected due to high, non-specific affinity of oligonucleotides for amyloid fibrils. Nevertheless, the high sensitivity, whereby aptamers detect beta-sheet formation, suggests that they can serve as superior amyloid recognition tools

GaN and InN Hexagonal Microdisks

The high-quality GaN microdisks with InGaN/GaN quantum wells (QWs) and InN microdisks were grown on γ-LiAlO2 substrates by plasma-assisted molecular beam epitaxy (PA-MBE). The samples were analysed using scanning electron microscopy, X-ray diffraction, photoluminescence, cathodoluminescence and high-resolution transmission electron microscope. The characteristics of the GaN microdisks and InN microdisks were studied and the effect of growth temperature was evaluated

Correlation between Pineal Activation and Religious Meditation Observed by Functional Magnetic Resonance Imaging

The human brain possesses plenty of functions but little is known about its scientific relationship with mind and spirit. Conferences^1,2^ focused on the connection between science and religion were held very recently in which neuroscientists, Buddhist scholars and Dalai Lama discussed attention, mental imagery, emotion, mind, brain functions and meditation, suggesting religious meditation offers an effective means to investigate the mystery of mind and spirit. In the past decade, scientists struggled to obtain brain mappings for various meditation styles using different brain imaging techniques and stimulating results have been observed^3-17^. In this letter we report that, together with other brain regions, pineal body exhibit significant activation during meditation process, supporting the long lasting speculation that pineal plays an important role in the intrinsic awareness which might concern spirit or soul. Pineal is known as an endocrine organ which produces substrates including melatonin and has been ascribed numerous even mysterious functions but its activation during meditation has never been observed by brain imaging technique. In seventeenth century, based on anatomic observation, Descartes ventured to suggest that pineal serves as the principal seat of the soul^18-20^. Inspired by its geometric center in the brain, physiologists, psychologists, philosophers and religionists have been speculating for centuries about pineal's function relevant to spirit and soul. In this study, we chose Chinese Original Quiet Sitting, one style of meditation, to explore this long lasting speculation by functional magnetic resonance imaging technique. Our results demonstrate a correlation between pineal activation and religious meditation which might have profound implications in physiological understanding of the intrinsic awareness

Rapid Growth of Galactic Supermassive Black Holes through Accreting Giant Molecular Clouds during Major Mergers of their Host Galaxies

Understanding the formation of the supermassive black holes (SMBHs) present in the centers of galaxies is a crucial topic in modern astrophysics. Observations have detected the SMBHs with mass $M$ of $10^{9}\, \rm M_\odot$ in the high-redshift galaxies with $\rm z\sim7$. However, how SMBHs grew to such huge masses within the first billion years after the big bang remains elusive. One possible explanation is that SMBHs grow quickly through the frequent mergers of galaxies, which provides sustainable gas to maintain rapid growth. This study presents the hydrodynamics simulations of the SMBHs' growth with their host galaxies using the GIZMO code. In contrast to previous simulations, we have developed a giant molecular cloud (GMC) model by separating molecular-gas particles from the atomic-gas particles and then evolving them independently. During major mergers, we show that the more massive molecular gas particles cloud bear stronger dynamical friction. Consequently, GMCs are substantially accreted onto the galactic centers that grow SMBHs from $\sim 10^{7}$ $\rm M_\odot$ to $\sim 10^{9}\, \rm M_\odot$ within $300$ Myr, explaining the rapid growth of SMBHs, and this accretion also triggers a violent starburst at the galactic center. Furthermore, we examine the impact of minor mergers on the bulge of a Milky-Way-like galaxy and find that the size and mass of the bulge can increase from $0.92$ kpc to $1.9$ kpc and from $4.7\times 10^{10}\, \rm M_\odot$ to $7\times 10^{10}\, \rm M_\odot$.Comment: 17 pages, 9 figures. Accepted for publication in Ap