3,569 research outputs found
Node synchronization schemes for the Big Viterbi Decoder
The Big Viterbi Decoder (BVD), currently under development for the DSN, includes three separate algorithms to acquire and maintain node and frame synchronization. The first measures the number of decoded bits between two consecutive renormalization operations (renorm rate), the second detects the presence of the frame marker in the decoded bit stream (bit correlation), while the third searches for an encoded version of the frame marker in the encoded input stream (symbol correlation). A detailed account of the operation is given, as well as performance comparison, of the three methods
Compressed/reconstructed test images for CRAF/Cassini
A set of compressed, then reconstructed, test images submitted to the Comet Rendezvous Asteroid Flyby (CRAF)/Cassini project is presented as part of its evaluation of near lossless high compression algorithms for representing image data. A total of seven test image files were provided by the project. The seven test images were compressed, then reconstructed with high quality (root mean square error of approximately one or two gray levels on an 8 bit gray scale), using discrete cosine transforms or Hadamard transforms and efficient entropy coders. The resulting compression ratios varied from about 2:1 to about 10:1, depending on the activity or randomness in the source image. This was accomplished without any special effort to optimize the quantizer or to introduce special postprocessing to filter the reconstruction errors. A more complete set of measurements, showing the relative performance of the compression algorithms over a wide range of compression ratios and reconstruction errors, shows that additional compression is possible at a small sacrifice in fidelity
Clumping Factor A Mediates Binding of Staphylococcus aureus to Human Platelets
The direct binding of bacteria to platelets may be an important virulence mechanism in the pathogenesis of infective endocarditis. We have previously described Staphylococcus aureus strain PS12, a Tn551-derived mutant of strain ISP479, with reduced ability to bind human platelets in vitro. When tested in an animal model of endocarditis, the PS12 strain was less virulent than its parental strain, as measured by bacterial densities in endocardial vegetations and incidence of systemic embolization. We have now characterized the gene disrupted in PS12 and its function in platelet binding. DNA sequencing, Southern blotting, and PCR analysis indicate that PS12 contained two Tn551 insertions within the clumping factor A (ClfA) locus (clfA). The first copy was upstream from the clfA start codon and appeared to have no effect on ClfA production. The second insertion was within the region encoding the serine aspartate repeat of ClfA and resulted in the production of a truncated ClfA protein that was secreted from the cell. A purified, recombinant form of the ClfA A region, encompassing amino acids 40 through 559, significantly reduced the binding of ISP479C to human platelets by 44% (P = 0.0001). Immunoprecipitation of recombinant ClfA that had been incubated with solubilized platelet membranes coprecipitated a 118-kDa platelet membrane protein. This protein does not appear to be glycoprotein IIb. These results indicate that platelet binding by S. aureus is mediated in part by the direct binding of ClfA to a novel 118-kDa platelet membrane receptor
Transposon Disruption of the Complex I NADH Oxidoreductase Gene (snoD) in Staphylococcus aureus Is Associated with Reduced Susceptibility to the Microbicidal Activity of Thrombin-Induced Platelet Microbicidal Protein 1
The cationic molecule thrombin-induced platelet microbicidal protein 1 (tPMP-1) exerts potent activity against Staphylococcus aureus. We previously reported that a Tn551 S. aureus transposon mutant, ISP479R, and two bacteriophage back-transductants, TxA and TxB, exhibit reduced in vitro susceptibility to tPMP-1 (tPMP-1(r)) compared to the parental strain, ISP479C (V. Dhawan, M. R. Yeaman, A. L. Cheung, E. Kim, P. M. Sullam, and A. S. Bayer, Infect. Immun. 65:3293-3299, 1997). In the current study, the genetic basis for tPMP-1(r) in these mutants was identified. GenBank homology searches using sequence corresponding to chromosomal DNA flanking Tn551 mutant strains showed that the fourth gene in the staphylococcal mnh operon (mnhABCDEFG) was insertionally inactivated. This operon was previously reported to encode a Na(+)/H(+) antiporter involved in pH tolerance and halotolerance. However, the capacity of ISP479R to grow at pH extremes and in high NaCl concentrations (1 to 3 M), coupled with its loss of transmembrane potential (DeltaPsi) during postexponential growth, suggested that the mnh gene products are not functioning as a secondary (i.e., passive) Na(+)/H(+) antiporter. Moreover, we identified protein homologies between mnhD and the nuo genes of Escherichia coli that encode components of a complex I NADH:ubiquinone oxidoreductase. Consistent with these data, exposures of tPMP-1-susceptible (tPMP-1(s)) parental strains (both clinical and laboratory derived) with either CCCP (a proton ionophore which collapses the proton motive force) or pieracidin A (a specific complex I enzyme inhibitor) significantly reduced tPMP-induced killing to levels seen in the tPMP-1(r) mutants. To reflect the energization of the gene products encoded by the mnh operon, we have renamed the locus sno (S. aureus nuo orthologue). These novel findings indicate that disruption of a complex I enzyme locus can confer reduced in vitro susceptibility to tPMP-1 in S. aureus
Order out of Randomness : Self-Organization Processes in Astrophysics
Self-organization is a property of dissipative nonlinear processes that are
governed by an internal driver and a positive feedback mechanism, which creates
regular geometric and/or temporal patterns and decreases the entropy, in
contrast to random processes. Here we investigate for the first time a
comprehensive number of 16 self-organization processes that operate in
planetary physics, solar physics, stellar physics, galactic physics, and
cosmology. Self-organizing systems create spontaneous {\sl order out of chaos},
during the evolution from an initially disordered system to an ordered
stationary system, via quasi-periodic limit-cycle dynamics, harmonic mechanical
resonances, or gyromagnetic resonances. The internal driver can be gravity,
rotation, thermal pressure, or acceleration of nonthermal particles, while the
positive feedback mechanism is often an instability, such as the
magneto-rotational instability, the Rayleigh-B\'enard convection instability,
turbulence, vortex attraction, magnetic reconnection, plasma condensation, or
loss-cone instability. Physical models of astrophysical self-organization
processes involve hydrodynamic, MHD, and N-body formulations of Lotka-Volterra
equation systems.Comment: 61 pages, 38 Figure
Impact of Vancomycin on sarA-Mediated Biofilm Formation: Role in Persistent Endovascular Infections Due to Methicillin-Resistant Staphylococcus aureus
Background. Staphylococcus aureus is the most common cause of endovascular infections. The staphylococcal accessory regulator A locus (sarA) is a major virulence determinant that may potentially impact methicillin-resistant S. aureus (MRSA) persistence in such infections via its influence on biofilm formation. Methods. Two healthcare-associated MRSA isolates from patients with persistent bacteremia and 2 prototypical community-acquired MRSA strains, as well as their respective isogenic sarA mutants, were studied for in vitro biofilm formation, fibronectin-binding capacity, autolysis, and protease and nuclease activities. These assays were done in the presence or absence of sub-minimum inhibitory concentrations (MICs) of vancomycin. In addition, these strain pairs were compared for intrinsic virulence and responses to vancomycin therapy in experimental infective endocarditis, a prototypical biofilm model. Results. All sarA mutants displayed significantly reduced biofilm formation and binding to fibronectin but increased protease production in vitro, compared with their respective parental strains. Interestingly, exposure to sub-MICs of vancomycin significantly promoted biofilm formation and fibronectin-binding in parental strains but not in sarA mutants. In addition, all sarA mutants became exquisitely susceptible to vancomycin therapy, compared with their respective parental strains, in the infective endocarditis model. Conclusions. These observations suggest that sarA activation is important in persistent MRSA endovascular infection, potentially in the setting of biofilm formatio
Color-Octet-Electroweak-Doublet Scalars and the CDF Dijet Anomaly
We study the phenomenology of color-octet scalars in the (8, 2)1/2
representation in the context of the 3.2\sigma excess, in the dijet invariant
mass spectrum of the W+jj final state, recently observed by the CDF
collaboration. We consider the region of parameter space with a sizable mass
splitting between the charged and neutral color-octet scalars and consistent
with electroweak precision data. We implement the principle of Minimal Flavor
Violation (MFV) in order to suppress FCNC currents and reduce the number of
free parameters. The excess in the W+jj channel corresponds to the charged
current decay of the heavier neutral octet scalar into its lighter charged
partner which decays into the two jets. In the MFV scenario, the production of
the neutral color-octet is dominated by gluon fusion due to the Yukawa
suppression of production via initial state quarks. As a result, no visible
excess is expected in the \gamma+jj channel due to Yukawa and CKM suppression.
Contributions to the Z+jj final state are suppressed for a mass spectrum where
the decay of the heavier color-octet to this final state is mediated by an
off-shell neutral color-octet partner. MFV allows one to control fraction of
bottom quarks in the final state jets by a single ratio of two free parameters.Comment: 14 pages, 6 figures, typos corrected, references added, text and
figures modified in some places for better clarity, version to appear in
Physics Letters
Higgs and Dark Matter Hints of an Oasis in the Desert
Recent LHC results suggest a standard model (SM)-like Higgs boson in the
vicinity of 125 GeV with no clear indications yet of physics beyond the SM. At
the same time, the SM is incomplete, since additional dynamics are required to
accommodate cosmological dark matter (DM). In this paper we show that
interactions between weak scale DM and the Higgs which are strong enough to
yield a thermal relic abundance consistent with observation can easily
destabilize the electroweak vacuum or drive the theory into a non-perturbative
regime at a low scale. As a consequence, new physics--beyond the DM
itself--must enter at a cutoff well below the Planck scale and in some cases as
low as O(10 - 1000 TeV), a range relevant to indirect probes of flavor and CP
violation. In addition, this cutoff is correlated with the DM mass and
scattering cross-section in a parameter space which will be probed
experimentally in the near term. Specifically, we consider the SM plus
additional spin 0 or 1/2 states with singlet, triplet, or doublet electroweak
quantum numbers and quartic or Yukawa couplings to the Higgs boson. We derive
explicit expressions for the full two-loop RGEs and one-loop threshold
corrections for these theories.Comment: 29 pages, 13 figure
Site-Specific Mutation of the Sensor Kinase GraS in Staphylococcus aureus Alters the Adaptive Response to Distinct Cationic Antimicrobial Peptides
The Staphylococcus aureus two-component regulatory system, GraRS, is involved in resistance to killing by distinct host defense cationic antimicrobial peptides (HD-CAPs). It is believed to regulate downstream target genes such as mprF and dltABCD to modify the S. aureus surface charge. However, the detailed mechanism(s) by which the histidine kinase, GraS, senses specific HD-CAPs is not well defined. Here, we studied a well-characterized clinical methicillin-resistant S. aureus (MRSA) strain (MW2), its isogenic graS deletion mutant (ΔgraS strain), a nonameric extracellular loop mutant (ΔEL strain), and four residue-specific ΔEL mutants (D37A, P39A, P39S, and D35G D37G D41G strains). The ΔgraS and ΔEL strains were unable to induce mprF and dltA expression and, in turn, demonstrated significantly increased susceptibilities to daptomycin, polymyxin B, and two prototypical HD-CAPs (hNP-1 and RP-1). Further, P39A, P39S, and D35G-D37G-D41G ΔEL mutations correlated with moderate increases in HD-CAP susceptibility. Reductions of mprF and dltA induction by PMB were also found in the ΔEL mutants, suggesting these residues are pivotal to appropriate activation of the GraS sensor kinase. Importantly, a synthetic exogenous soluble EL mimic of GraS protected the parental MW2 strain against hNP-1- and RP-1-mediated killing, suggesting a direct interaction of the EL with HD-CAPs in GraS activation. In vivo, the ΔgraS and ΔEL strains displayed dramatic reductions in achieved target tissue MRSA counts in an endocarditis model. Taken together, our results provide new insights into potential roles of GraS in S. aureus sensing of HD-CAPs to induce adaptive survival responses to these molecules
The GraS Sensor in Staphylococcus Aureus Mediates Resistance to Host Defense Peptides Differing in Mechanisms of Action
Staphylococcus aureus uses the two-component regulatory system GraRS to sense and respond to host defense peptides (HDPs). However, the mechanistic impact of GraS or its extracellular sensing loop (EL) on HDP resistance is essentially unexplored. Strains with null mutations in the GraS holoprotein (ΔgraS) or its EL (ΔEL) were compared for mechanisms of resistance to HDPs of relevant immune sources: neutrophil α-defensin (human neutrophil peptide 1 [hNP-1]), cutaneous β-defensin (human β-defensin 2 [hBD-2]), or the platelet kinocidin congener RP-1. Actions studied by flow cytometry included energetics (ENR); membrane permeabilization (PRM); annexin V binding (ANX), and cell death protease activation (CDP). Assay conditions simulated bloodstream (pH 7.5) or phagolysosomal (pH 5.5) pH contexts. S. aureus strains were more susceptible to HDPs at pH 7.5 than at pH 5.5, and each HDP exerted a distinct effect signature. The impacts of ΔgraS and ΔΕL on HDP resistance were peptide and pH dependent. Both mutants exhibited defects in ANX response to hNP-1 or hBD-2 at pH 7.5, but only hNP-1 did so at pH 5.5. Both mutants exhibited hyper-PRM, -ANX, and -CDP responses to RP-1 at both pHs and hypo-ENR at pH 5.5. The actions correlated with ΔgraS or ΔΕL hypersusceptibility to hNP-1 or RP-1 (but not hBD-2) at pH 7.5 and to all study HDPs at pH 5.5. An exogenous EL mimic protected mutant strains from hNP-1 and hBD-2 but not RP-1, indicating that GraS and its EL play nonredundant roles in S. aureus survival responses to specific HDPs. These findings suggest that GraS mediates specific resistance countermeasures to HDPs in immune contexts that are highly relevant to S. aureus pathogenesis in humans
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