107 research outputs found
Functional modes of proteins are among the most robust ones
It is shown that a small subset of modes which are likely to be involved in
protein functional motions of large amplitude can be determined by retaining
the most robust normal modes obtained using different protein models. This
result should prove helpful in the context of several applications proposed
recently, like for solving difficult molecular replacement problems or for
fitting atomic structures into low-resolution electron density maps. Moreover,
it may also pave the way for the development of methods allowing to predict
such motions accurately.Comment: 4 pages, 5 figure
Markov propagation of allosteric effects in biomolecular systems: application to GroEL–GroES
We introduce a novel approach for elucidating the potential pathways of allosteric communication in biomolecular systems. The methodology, based on Markov propagation of ‘information' across the structure, permits us to partition the network of interactions into soft clusters distinguished by their coherent stochastics. Probabilistic participation of residues in these clusters defines the communication patterns inherent to the network architecture. Application to bacterial chaperonin complex GroEL–GroES, an allostery-driven structure, identifies residues engaged in intra- and inter-subunit communication, including those acting as hubs and messengers. A number of residues are distinguished by their high potentials to transmit allosteric signals, including Pro33 and Thr90 at the nucleotide-binding site and Glu461 and Arg197 mediating inter- and intra-ring communication, respectively. We propose two most likely pathways of signal transmission, between nucleotide- and GroES-binding sites across the cis and trans rings, which involve several conserved residues. A striking observation is the opposite direction of information flow within cis and trans rings, consistent with negative inter-ring cooperativity. Comparison with collective modes deduced from normal mode analysis reveals the propensity of global hinge regions to act as messengers in the transmission of allosteric signals
Crafts and gear used for marine fishing along the Andhra Pradesh coast
Until the middle of nineteen sixties fishing
for marine finfishes and shellfishes along the
Andhra Pradesh coast used to be carried out
employing indigenous non-mechanised crafts.
Subsequently trawlers and later mechanised
vessels operating gillnets came into use which
resulted in the increased fish production. Use
of outboard engine on indigenous crafts for
reaching fishing grounds is a recent feature of
near the coast fishing. The different kinds of
non-mechanised and mechanised crafts and
gear employed in the region are dealt with in
this article
Seaweed resources off Tamil Nadu coast: Sector III. Valinokkam - Kilakkarai
Survey of seaweed resources in deep water was carried out in the area between Valinokkam and Kilakkarai during January 1989 and March,l990 respectively. In the survey from Valinokkam to Kilakkarai 33 species of marine algae were recorded of which 8 species belong to Chlorophyta, 8 to Phaeophyta and 17 to Rhodophyta. Only one species of seagrass Halophila ovalis was recorded. Among the 200 sq Icm area surveyed, vegetation occurred only in 27.5 sq.km with a total standing crop of 2962.5 tonnes (wet wt)
Seaweed resources off Tamil nadu coast, Sector - IV Kilakkarai - Rameswaram island (Dhanushkodi
Survey of the deep water area from Kilakkarai (Appa Tivu to Rameswaram Island
(Dhanushkodi) was undertaken from December 1990 to January 1991 in the IV Sector survey
of Deep water Seaweed Resources off Tamil Nadu coast, which formed the last phase of the
survey from Rameswaram to Kanyakumari carried out during 1986-91. An area of 417 .5 sq.km.
was surveyed yielding a total biomass of 18,162.5 tons (wet) seaweeds. Out of 167 stations
surveyed in 13 transects, vegetation occurred only in 12 stations. Of the 29 species of marine
algae recorded 8 belonged to Chlorophyta, 8 to Phaeophyta, 12 to Rhodophyta, and I to
Cyanophyta, One species of seagrass Cymodocea serrulata was also recorded. Twenty species
were found in estimable quantities, of which the following 8 species were abundant: Halimeda
macr%ba, Spatog/ossum asperum, Zonaria crenata, Sargassum ilicijolium, Amphiroa
jragilissima, Hypnea muscijormis, Botryocladia /eplOpoda and Lyngbya majuscula with a biomass
of 1325, 9775, 650, 1550, 1925,300,862.5 and 1012.5 tons (wet) respectively. The species of
Hypnea and Sargassum could be exploited for the manufacture of phytochemicals
On the conservation of the slow conformational dynamics within the amino acid kinase family: NAGK the paradigm
N-Acetyl-L-Glutamate Kinase (NAGK) is the structural paradigm for examining the catalytic mechanisms and dynamics of amino acid kinase family members. Given that the slow conformational dynamics of the NAGK (at the microseconds time scale or slower) may be rate-limiting, it is of importance to assess the mechanisms of the most cooperative modes of motion intrinsically accessible to this enzyme. Here, we present the results from normal mode analysis using an elastic network model representation, which shows that the conformational mechanisms for substrate binding by NAGK strongly correlate with the intrinsic dynamics of the enzyme in the unbound form. We further analyzed the potential mechanisms of allosteric signalling within NAGK using a Markov model for network communication. Comparative analysis of the dynamics of family members strongly suggests that the low-frequency modes of motion and the associated intramolecular couplings that establish signal transduction are highly conserved among family members, in support of the paradigm sequence→structure→dynamics→function © 2010 Marcos et al
Signal Transduction Pathways in the Pentameric Ligand-Gated Ion Channels
The mechanisms of allosteric action within pentameric ligand-gated ion channels (pLGICs) remain to be determined. Using crystallography, site-directed mutagenesis, and two-electrode voltage clamp measurements, we identified two functionally relevant sites in the extracellular (EC) domain of the bacterial pLGIC from Gloeobacter violaceus (GLIC). One site is at the C-loop region, where the NQN mutation (D91N, E177Q, and D178N) eliminated inter-subunit salt bridges in the open-channel GLIC structure and thereby shifted the channel activation to a higher agonist concentration. The other site is below the C-loop, where binding of the anesthetic ketamine inhibited GLIC currents in a concentration dependent manner. To understand how a perturbation signal in the EC domain, either resulting from the NQN mutation or ketamine binding, is transduced to the channel gate, we have used the Perturbation-based Markovian Transmission (PMT) model to determine dynamic responses of the GLIC channel and signaling pathways upon initial perturbations in the EC domain of GLIC. Despite the existence of many possible routes for the initial perturbation signal to reach the channel gate, the PMT model in combination with Yen's algorithm revealed that perturbation signals with the highest probability flow travel either via the β1-β2 loop or through pre-TM1. The β1-β2 loop occurs in either intra- or inter-subunit pathways, while pre-TM1 occurs exclusively in inter-subunit pathways. Residues involved in both types of pathways are well supported by previous experimental data on nAChR. The direct coupling between pre-TM1 and TM2 of the adjacent subunit adds new insight into the allosteric signaling mechanism in pLGICs. © 2013 Mowrey et al
Scaling of mean first-passage time as efficiency measure of nodes sending information on scale-free Koch networks
A lot of previous work showed that the sectional mean first-passage time
(SMFPT), i.e., the average of mean first-passage time (MFPT) for random walks
to a given hub node (node with maximum degree) averaged over all starting
points in scale-free small-world networks exhibits a sublinear or linear
dependence on network order (number of nodes), which indicates that hub
nodes are very efficient in receiving information if one looks upon the random
walker as an information messenger. Thus far, the efficiency of a hub node
sending information on scale-free small-world networks has not been addressed
yet. In this paper, we study random walks on the class of Koch networks with
scale-free behavior and small-world effect. We derive some basic properties for
random walks on the Koch network family, based on which we calculate
analytically the partial mean first-passage time (PMFPT) defined as the average
of MFPTs from a hub node to all other nodes, excluding the hub itself. The
obtained closed-form expression displays that in large networks the PMFPT grows
with network order as , which is larger than the linear scaling of
SMFPT to the hub from other nodes. On the other hand, we also address the case
with the information sender distributed uniformly among the Koch networks, and
derive analytically the entire mean first-passage time (EMFPT), namely, the
average of MFPTs between all couples of nodes, the leading scaling of which is
identical to that of PMFPT. From the obtained results, we present that although
hub nodes are more efficient for receiving information than other nodes, they
display a qualitatively similar speed for sending information as non-hub nodes.
Moreover, we show that the location of information sender has little effect on
the transmission efficiency. The present findings are helpful for better
understanding random walks performed on scale-free small-world networks.Comment: Definitive version published in European Physical Journal
Major and minor fisheries harbours of India. 8. Fisherries harbours along the coasts of North Andhra Pradesh, Orissa and West Bengal
The present account gives information about the three major and five minor fisheries harbours along the coasts of north Andhra Pradesh, Orissa and West Benga
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