Age-associated microbiome shows the giant panda lives on hemicelluloses, not on cellulose

The giant panda feeds almost exclusively on bamboo, a diet highly enriched in lignin and cellulose, but is characterized by a digestive tract similar to carnivores. It is still large unknown if and how the giant panda gut microbiota contributes to lignin and cellulose degradation. Here we show the giant pandas’ gut microbiota does not significantly contribute to cellulose and lignin degradation. We found that no operational taxonomic unit had a nearest neighbor identified as a cellulolytic species or strain with a significant higher abundance in juvenile than cubs, a very low abundance of putative lignin and cellulose genes existed in part of analyzing samples but a significant higher abundance of genes involved in starch and hemicellulose degradation in juveniles than cubs. Moreover, a significant lower abundance of putative cellulolytic genes and a significant higher abundance of putative α-amylase and hemicellulase gene families were present in giant pandas than in omnivores or herbivores

CLAP-PRE: Certificateless Autonomous Path Proxy Re-Encryption for Data Sharing in the Cloud

In e-health systems, patients encrypt their personal health data for privacy purposes and upload them to the cloud. There exists a need for sharing patient health data with doctors for healing purposes in one’s own preferred order. To achieve this fine-gained access control to delegation paths, some researchers have designed a new proxy re-encryption (PRE) scheme called autonomous path proxy re-encryption (AP-PRE), where the delegator can control the whole delegation path in a multi-hop delegation process. In this paper, we introduce a certificateless autonomous path proxy re-encryption (CLAP-PRE) using multilinear maps, which holds both the properties (i.e., certificateless, autonomous path) of certificateless encryption and autonomous path proxy re-encryption. In the proposed scheme, (a) each user has two public keys (user’s identity and traditional public key) with corresponding private keys, and (b) each ciphertext is first re-encrypted from a public key encryption (PKE) scheme to an identity-based encryption (IBE) scheme and then transformed in the IBE scheme. Our scheme is an IND-CPA secure CLAP-PRE scheme under the k-multilinear decisional Diffie–Hellman (k-MDDH) assumption in the random oracle model

CLAP-PRE: Certificateless Autonomous Path Proxy Re-Encryption for Data Sharing in the Cloud

In e-health systems, patients encrypt their personal health data for privacy purposes and upload them to the cloud. There exists a need for sharing patient health data with doctors for healing purposes in one’s own preferred order. To achieve this fine-gained access control to delegation paths, some researchers have designed a new proxy re-encryption (PRE) scheme called autonomous path proxy re-encryption (AP-PRE), where the delegator can control the whole delegation path in a multi-hop delegation process. In this paper, we introduce a certificateless autonomous path proxy re-encryption (CLAP-PRE) using multilinear maps, which holds both the properties (i.e., certificateless, autonomous path) of certificateless encryption and autonomous path proxy re-encryption. In the proposed scheme, (a) each user has two public keys (user’s identity and traditional public key) with corresponding private keys, and (b) each ciphertext is first re-encrypted from a public key encryption (PKE) scheme to an identity-based encryption (IBE) scheme and then transformed in the IBE scheme. Our scheme is an IND-CPA secure CLAP-PRE scheme under the k-multilinear decisional Diffie–Hellman (k-MDDH) assumption in the random oracle model

Dual-laser-induced breakdown thermometry via sound speed measurement： A new procedure for improved spatiotemporal resolution

Measurement of acoustic waves from laser-induced breakdown has been developed as gas thermometry in combustion atmospheres. In the measurement, two laser-induced breakdown spots are generated and the local gas temperature between these two spots is determined through the measurement of the sound speed between them. In the previous study, it was found that the local gas breakdown can introduce notable system uncertainty, about 5% to the measured temperature. To eliminate the interference, in present work, a new measurement procedure was proposed, where two individual laser pulses with optimized firing order and delay time were employed. With the new measurement procedure, the system uncertainty caused by local gas breakdown can be largely avoided and the temporal and spatial resolutions can reach up to 0.5 ms and 10 mm, respectively. The improved thermometry, dual-laser-induced breakdown thermometry (DLIBT), was applied to measure temperatures of hot flue gases provided by a multijet burner. The measured temperatures covering the range between 1000 K and 2000 K were compared with the ones accurately obtained through the two-line atomic fluorescence (TLAF) thermometry with a measurement uncertainty of ~3%, and a very good agreement was obtained

Numerical Study on Thermal Stress of High Temperature Proton Exchange Membrane Fuel Cells during Start-Up Process

High-temperature proton-exchange membrane fuel cells (HT-PEMFCs) with phosphoric-doped polybenzimidazole (PBI) membranes have a higher operating temperature compared to the PEMFCs operating below 373.15 K. The fuel cell is first heated from room temperature to the minimum operating temperature to avoid the generation of liquid water. The existence of liquid water can result in the loss of phosphoric acid and then affect the cell performance. In this study, the start-up process of HT-PEMFCs is numerically studied by establishing a three-dimensional non-isothermal mathematical model. Preheated gas is supplied into gas flow channels to heat the fuel cell, and then voltage load is applied to accelerate the start-up process. Effects of voltage (0.9 V, 0.7 V and 0.5 V) and flow arrangement (co-flow and counter flow) on temperature, current density, proton conductivity and stress distributions of fuel cells are examined. It is found that the maximum stress is increased when a lower voltage is adopted, and the counter-flow arrangement provides a more uniform stress distribution than that of co-flow arrangement

Numerical simulation of inertia friction welding process of GH4169 alloy

Friction welding is a solid state welding technology with good quality and high automation. It has been widely used in many industry fields especially in automobile and aerospace industry. Because of the characters of less process parameters and high automation, inertia friction welding is popular in many fields. In this paper, a 2-D thermo-mechanical FEM model was developed to simulate inertia welding process. In this model, the temperature dependency of the thermal and mechanical properties of material was considered. The finite-element software MSC.Marc was used to calculate the temperature field, stress field and strain field during inertia friction welding process. The transient temperature field and the deformation of GH4169 superalloy during inertia friction welding process were predicted. The temperature filed during inertia friction welding process was measured by means of thermocouples. The calculated temperature filed is in good agreement with the experimental result

First Discovering of Nanoscale Tectonics in Western of Qiongnan Paleo-Tethyan Suture Zone in North Margin of South China Sea and Its Geotectonic Significance

The western part of Qiongnan Paleo-Tethyan Suture Zone in the northern margin of the South China Sea is commonly known as the Jiusuo-Lingshui fault zone. At first, it was deduced as a near EW-direction tectonic boundary zone based on the data of geophysical field. Due to the lack of direct evidence of tectonic deformation, the existence and geological significance of the boundary zone have been controversial for a long time. Through field observation, indoor polarizing microscope and SEM (scanning electron microscope) tests on the basement rocks exposed along the near NS-direction profile of Xiaomei riverbed, Duzong reservoir, Junchang bridge, and Lipen reservoir, and Hainan Island, the authors discovered ductile shear structure in the boundary zone, crystalline quartz schist, granitic gneiss, meta-quartz sandstone, and porphyritic quartz rock. The sha = ear structures were formed by regional dynamic-thermal metamorphism and are the most common ductile deformation metamorphic rock in general orogenic belt. Furthermore, the microstructure and nano structure, which often developed in the ductile shear zone, were found through microscope and SEM. Moreover, the authors discussed nanoscale characteristics of Xiaomei ductile shear zone in Hainan Island. The study results showed that the three rocks of granite, quartz schist and granitic gneiss have many kinds of nano textures and structures. Based on field actual situation of the development degree of nano particles in this shear zone, it is known that these nano textures and structures are related to shearing action. Their formation mechanism may be as follows: one is thermal decomposition of layered silicate under shearing action, the other is brittle fracture after particle plastic deformation and grinding through shearing action. Based on analysis of the Xiaomei ductile shear zone, and after comparison of SEM test results carried out to the rock samples of ductile shear zone of Taroko deep fault zone in Taiwan, we found that the characteristics of the nano particles and ones in Xiaomei ductile shear zone are comparable. According to their relations with the regional deep fault, it could be speculated that Xiaomei ductile shear zone may be closely correlated with Jiusuo-Lingshui deep fault zone in the regional tectonic belonging. These study results can provide important evidence for determination of the tectonic property and spatial position of Jiusuo-Lingshui fault zone

Data from: Transcriptome-derived tetranucleotide microsatellites and their associated genes from the giant panda (Ailuropoda melanoleuca)

Recently, an increasing number of microsatellites or Simple Sequence Repeats (SSRs) have been found and characterized from transcriptome. Such SSRs can be employed as putative functional markers to easily tag corresponding genes, which play an important role in biomedical studies and genetic analysis. However, the transcriptome-derived SSRs for giant panda (Ailuropoda melanoleuca) are not yet available. In the present work, we identified and characterized 20 tetranucleotide microsatellite loci from a transcript database generated from the blood of giant panda. Furthermore, we assigned their predicted transcriptome locations: 16 loci were assigned to untranslated regions (UTRs) and 4 loci were assigned to coding regions (CDSs). Gene identities of 14 transcripts contained corresponding microsatellites were determined, which provide useful information to study the potential contribution of SSRs to gene regulation in giant panda. The polymorphic information content (PIC) values ranged from 0.293 to 0.789 with an average of 0.603 for the 16 UTRs-derived SSRs. Interestingly, four CDS-derived microsatellites developed in our study were also polymorphic, and the instability of these four CDS-derived SSRs was further validated by re-genotyping and sequencing. The genes contained these four CDS-derived SSRs were embedded with various types of repeat motifs. The interaction of all the length-changing SSRs might provide a way against coding region frameshift caused by microsatellite instability. We hope these newly gene-associated biomarkers would pave the way for genetic and biomedical studies for giant panda in the future. In sum, this set of transcriptome-derived markers complements the genetic resources available for giant panda

Causal role of immune cells in schizophrenia: Mendelian randomization (MR) study

Abstract Background Complex immune-brain interactions that affect neural development, survival and function might have causal and therapeutic implications for psychiatric illnesses. However, previous studies examining the association between immune inflammation and schizophrenia (SCZ) have yielded inconsistent findings. Methods Comprehensive two-sample Mendelian randomization (MR) analysis was performed to determine the causal association between immune cell signatures and SCZ in this study. Based on publicly available genetic data, we explored causal associations between 731 immune cell signatures and SCZ risk. A total of four types of immune signatures (median fluorescence intensities (MFI), relative cell (RC), absolute cell (AC), and morphological parameters (MP)) were included. Comprehensive sensitivity analyses were used to verify the robustness, heterogeneity, and horizontal pleiotropy of the results. Results After FDR correction, SCZ had no statistically significant effect on immunophenotypes. It was worth mentioning some phenotypes with unadjusted low P-values, including FSC-A on NKT (β = 0.119, 95% CI = 0.044 ~ 0.194, P = 0.002), DN (CD4-CD8-) NKT %T cell (β = 0.131, 95% CI = 0.054 ~ 0.208, P = 9.03 × 10− 4), and SSC-A on lymphocytes (β = 0.136, 95% CI = 0.059 ~ 0.213, P = 5.43 × 10− 4). The causal effect of SCZ IgD on transitional was estimated to 0.127 (95% CI = 0.051 ~ 0.203, P = 1.09 × 10− 3). SCZ also had a causal effect on IgD+ %B cell (β = 0.130, 95% CI = 0.054 ~ 0.207, P = 8.69 × 10− 4), and DP (CD4+CD8+) %T cell (β = 0.131, 95% CI = 0.054 ~ 0.207, P = 8.05 × 10− 4). Furthermore, four immunophenotypes were identified to be significantly associated with SCZ risk: naive CD4+ %T cell (OR = 0.986, 95% CI = 0.979 ~ 0.992, P = 1.37 × 10− 5), HLA DR on CD14− CD16− (OR = 0.738 (95% CI = 0.642 ~ 0.849, P = 2.00 × 10− 5), CD33dim HLA DR+ CD11b− AC (OR = 0.631, 95% CI = 0.529 ~ 0.753, P = 3.40 × 10− 7) and activated & resting Treg % CD4 Treg (OR = 0.937, 95% CI = 0.906 ~ 0.970, P = 1.96 × 10− 4). Conclusions Our study has demonstrated the close connection between immune cells and SCZ by genetic means, thus providing guidance for future clinical research