Graduate Recital: Chieh-Chun Chen, Piano

Kemp Recital Hall April 20, 2019 Saturday Morning 11:30 a.m

Identification of Novel (\u3cem\u3eR\u3c/em\u3eNAi \u3cem\u3eDe\u3c/em\u3eficient) Genes in \u3cem\u3eC. elegans\u3c/em\u3e: A Dissertation

RNA interference or RNAi was first discovered as an experimental approach that induces potent sequence-specific gene silencing. Remarkably, subsequent studies on dissecting the molecular mechanism of the RNAi pathway reveal that RNAi is conserved in most eukaryotes. In addition, genes and mechanisms related to RNAi are employed to elicit the regulation of endogenous gene expression that controls a variety of important biological processes. To investigate the mechanism of RNAi in the nematode C. elegans, we performed genetic screens in search of RNAi deficient mutants (rde). Here I report the summary of the genetic screens in search of rde mutants as well as the identification of two novel genes required for the RNAi pathway, rde-3 and rde-8. In addition, we demonstrate that some of the rde genes, when mutated, render the animals developmentally defective, suggesting that these rde genes also function in developmental gene regulation. This work presents novel insights on the components of the RNAi pathway and the requirement of these components in the regulation of endogenous gene expression

Monotherapy of acetylsalicylic acid or warfarin for prevention of ischemic stroke in low-risk atrial fibrillation: A Easter Asian population-based study

Background: This study aimed to investigate the effectiveness of monotherapy acetylsalicylic acid(ASA) and warfarin for stroke prevention in low-risk atrial fibrillation (AF) by using a population--based cohort study in Taiwan.Methods: A newly diagnosed low-risk AF patient cohort were identified by using National HealthInsurance Research Database (NHIRD) in Taiwan in 2008. The study cohort was observed witha follow-up of 2 years to examine the onset of ischemic stroke (IS) (to 2010). The longitudinal data wereanalyzed by using generalized estimation equations (GEE).Results: A total of 8,065 newly-diagnosed low-risk AF patients were identified in 2008. 7.4% wereprescribed with ASA and 4.6% were prescribed with warfarin. The GEE results showed that low-riskAF patients with hypertension who received warfarin were associated with a statistically significant58.4% reduction of IS risk (OR = 0.416, p = 0.024, 95% CI 0.194–0.891). Additionally, low-risk AFpatients with hyperlipidemia who received warfarin were associated with a 69.3% reduction of IS risk(OR = 0.307, p = 0.044, 95% CI 0.097–0.969).Conclusions: Warfarin is suggested to be prescribed in preventing IS for low-stroke-risk AF patientswith hypertension and hyperlipidemia

Selection of thermodynamic models for combinatorial control of multiple transcription factors in early differentiation of embryonic stem cells

<p>Abstract</p> <p>Background</p> <p>Transcription factors (TFs) have multiple combinatorial forms to regulate the transcription of a target gene. For example, one TF can help another TF to stabilize onto regulatory DNA sequence and the other TF may attract RNA polymerase (RNAP) to start transcription; alternatively, two TFs may both interact with both the DNA sequence and the RNAP. The different forms of TF-TF interaction have different effects on the probability of RNAP's binding onto the promoter sequence and therefore confer different transcriptional efficiencies.</p> <p>Results</p> <p>We have developed an analytical method to identify the thermodynamic model that best describes the form of TF-TF interaction among a set of TF interactions for every target gene. In this method, time-course microarray data are used to estimate the steady state concentration of the transcript of a target gene, as well as the relative changes of the active concentration for each TF. These estimated concentrations and changes of concentrations are fed into an inference scheme to identify the most compatible thermodynamic model. Such a model represents a particular way of combinatorial control by multiple TFs on a target gene.</p> <p>Conclusions</p> <p>Applying this approach to a time-course microarray dataset of embryonic stem cells, we have inferred five interaction patterns among three regulators, Oct4, Sox2 and Nanog, on ten target genes.</p

Two dimensional shape measurement via video signal processing

Thesis (M.S.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 1978.Includes bibliographical references.by Chun-Chieh Chen.M.S

Interactions Between Reinforcement Corrosion and Chloride Ion Diffusion in Mortar

This study explored the diffusion of the chloride ions influenced by the reinforcement corrosion in the mortar. It is believed that, during the corroding process, a small current is generated at the surface of the reinforcement. Such current is supposed to influence the diffusion of the chloride ions, but the relationship between both was not well studied in the literature. In this study, the corroded reinforcements were prepared by applied currents. Reinforced mortar specimens with w/c of 0.6 were then prepared and cured by either salt or fresh water. Results showed that the chloride ion distribution was likely associated with the reinforcement corrosion. During the early hydration, the chloride ions were attracted by the reinforcement corrosion in the specimens prepared with fresh water and cured in salt water. The concentration of the chloride ions near the surface of the reinforcement was increased with the increases of the charging time during the preparation for the corroded reinforcement. On the contrary, the chloride ions were likely bound in those specimens prepared with salt water and cured by saturated lime water. The concentration of the chloride ions near the surface of the reinforcement was higher than those near the outer surface. However, such influencing effects were not clear in the long term, possibly due to the hydration. The results of this study show that the reinforcement corrosion have influences on the diffusion of the chloride ions and such effect should be considered during the refinement of the traditional chloride ion diffusion models

Impacts of Techno-Dependence in The Mobile Instant Messaging Environment

Mobile Instant Messaging (MIM) services such as LINE, WhatsApp, Facebook Messenger and WeChat have already established a mobile communication environment that extends beyond words and sounds. As MIM provides an effective way to communicate, it can improve workplace efficiency, whether within an organization or among offices spread around the world. Nowadays, MIM has been widely accepted as both a social and work tool. The social interaction overload generated by SNSs contributes to emotional exhaustion. Emotional exhaustion, on the other hand, leads to dissatisfaction and discontinuous usage intentions. In the context of MIMs (since they are relative new apps), users whether are likely to experience emotional exhaustion that is generated by social interaction overload, and therefore discontinue their use of MIMs. In contrast to traditional research on IS continued use in the past which defines dependence as a routine and unconscious usage pattern. MIMs offer a communication method that is faster and easier than phone calls or SMS. It is possible that MIMs bring people closer by allowing their users to understand more of the situational matters related to their friends or family, without being limited by distance. Specifically, social-group functions offered by LINE can encourage users to join certain social groups (for instance, family, colleagues, classmates, or friends). Group members can not only discuss common topics, they can also share their “photo albums,” enabling members to enhance their sense of belonging. At the same time, they have the opportunity to feel a sense of being valued, loved, and needed. Although the mobility and accessibility of mobile devices allow users to instantly contact each other on MIMs and on real-time basis, excessive use of MIMs, or MIM techno-dependence, is likely to generate social-related stress among their users. Therefore, this research attempts to explore the possibility that MIM techno-dependency can have non-detrimental effects, and considers the positive and healthy results from MIM techno-dependency due to an increased sense of belonging. The questions explored include: do MIMs users develop a positive techno-dependence? Does this positive emotional reaction encourage MIMs users to continue their use of MIMs? Since LINE is a relative newcomer to MIM, there is still a dearth of research needed to explore issues related to using MIM as a research tool. This study considers how LINE combines a diverse range of communication approaches—such as voice, texts, maps, pictures, photos, locations, video, and audio—with a variety of community groups such as friends chat, group chat, dynamic news, and official accounts. It seems worthwhile to study characteristics of LINE’s users in order to further explore the issues related to MIM. Through the hypotheses development and a survey research on 685 LINE users, this study inferred that users make frequent use of LINE in the long-term mainly because of four kinds of techno-dependence: people, fun, information, and work. Such techno-dependence generates positive and negative consequences concurrently. On the one hand, because the user’s dependence on LINE enhances his or her belongingness through friends, colleagues, and family, this positive social and emotional reaction will make users satisfied with LINE, and thus increase continuous usage intention for LINE. On the other hand, the user’s dependence on LINE means that they experience social interaction overload resulting in emotional exhaustion. Dependence on LINE leads to users experiencing pressure from both social message overload and social demand overload, resulting in social interaction overload. This negative social and emotional reaction will cause a decrease in user satisfaction with LINE, thereby reducing the continuous usage intention of LINE. Based on these findings, we suggest that LINE-related techno-dependence can enable users to increase their sense of positive social belongingness, but can also cause negative social interaction overload. It is concluded that the consequences of techno-dependence are characterized by both positive and negative emotions. Users’ evaluations of LINE are simultaneously affected by positive and negative social and emotional factors