Impact environnemental des pneumatiques déchiquetés utilisés pour la construction d’ouvrages en remblai

Les pneumatiques usagés sont employés comme matériau de construction pour des ouvrages de génie civil et de géotechnique depuis les années 80. S’agissant de déchets, leur impact sur l’environnement doit être évalué, au regard de l’application visée et de la réglementation en vigueur. Cet article porte sur l’impact environnemental de pneumatiques usagés déchiquetés, lorsqu'ils sont utilisés en mélange avec du sable, pour la construction d’ouvrages en remblai. Des essais de lixiviation, de percolation et au lysimètre ont été réalisés, sur le matériau vierge et sur son résidu après incendie. Les liquides collectés ont été l’objet d’analyses physico-chimiques, considérant deux cents composés, et écotoxicologiques sur une bactérie et un crustacé. Les mesures révèlent un impact très limité en contexte normal. Par contre, les résidus après incendie doivent être envoyés en installation de stockage pour déchets dangereux. / This article deals with the assessment of the environmental impact of shredded scrap tyres mixed with sand to built embankments. Tests of leaching, of percolation and in a lysimeter were performed on the raw material as well as on the tailing resulting from fire. The liquids were analysed looking for 200 compounds and their toxicity on a shellfish and a bacterium was assessed. Measurements confirm the limited consequences on the environment, except in the case of fire, where tailings must be considered as hazardous waste

Produção de hidrogénio solar com simultânea mineralização de poluentes orgânicos

A produção foto-catalítica de hidrogénio por iluminação de uma suspensão de nanopartículas de óxidos semicondutores apresenta vantagens atractivas sobre outros métodos de elevado custo, como a electrólise. Como barreira tecnológica à aplicação comercial daquela tecnologia salienta-se a sua baixa eficiência. Neste trabalho demonstra-se o aumento na eficiência da produção foto-catalítica de hidrogénio a partir de água, utilizando poluentes orgânicos como agentes sacrificiais. Os catalisadores desenvolvidos permitem também estender a aplicação do processo à utilização de luz solar directa ou luz artificial, fundamental para o avanço e implementação da tecnologi

Pre-M Phase-promoting Factor Associates with Annulate Lamellae in Xenopus Oocytes and Egg Extracts

We have used complementary biochemical and in vivo approaches to study the compartmentalization of M phase-promoting factor (MPF) in prophase Xenopus eggs and oocytes. We first examined the distribution of MPF (Cdc2/CyclinB2) and membranous organelles in high-speed extracts of Xenopus eggs made during mitotic prophase. These extracts were found to lack mitochondria, Golgi membranes, and most endoplasmic reticulum (ER) but to contain the bulk of the pre-MPF pool. This pre-MPF could be pelleted by further centrifugation along with components necessary to activate it. On activation, Cdc2/CyclinB2 moved into the soluble fraction. Electron microscopy and Western blot analysis showed that the pre-MPF pellet contained a specific ER subdomain comprising "annulate lamellae" (AL): stacked ER membranes highly enriched in nuclear pores. Colocalization of pre-MPF with AL was demonstrated by anti-CyclinB2 immunofluorescence in prophase oocytes, in which AL are positioned close to the vegetal surface. Green fluorescent protein-CyclinB2 expressed in oocytes also localized at AL. These data suggest that inactive MPF associates with nuclear envelope components just before activation. This association may explain why nuclei and centrosomes stimulate MPF activation and provide a mechanism for targeting of MPF to some of its key substrates

Eccentric Exercise Activates Novel Transcriptional Regulation of Hypertrophic Signaling Pathways Not Affected by Hormone Changes

Unaccustomed eccentric exercise damages skeletal muscle tissue, activating mechanisms of recovery and remodeling that may be influenced by the female sex hormone 17β-estradiol (E2). Using high density oligonucleotide based microarrays, we screened for differences in mRNA expression caused by E2 and eccentric exercise. After random assignment to 8 days of either placebo (CON) or E2 (EXP), eighteen men performed 150 single-leg eccentric contractions. Muscle biopsies were collected at baseline (BL), following supplementation (PS), +3 hours (3H) and +48 hours (48H) after exercise. Serum E2 concentrations increased significantly with supplementation (P<0.001) but did not affect microarray results. Exercise led to early transcriptional changes in striated muscle activator of Rho signaling (STARS), Rho family GTPase 3 (RND3), mitogen activated protein kinase (MAPK) regulation and the downstream transcription factor FOS. Targeted RT-PCR analysis identified concurrent induction of negative regulators of calcineurin signaling RCAN (P<0.001) and HMOX1 (P = 0.009). Protein contents were elevated for RND3 at 3H (P = 0.02) and FOS at 48H (P<0.05). These findings indicate that early RhoA and NFAT signaling and regulation are altered following exercise for muscle remodeling and repair, but are not affected by E2

Site-specific organism vs ROs/RAPs an improvement of the environmental risk assessment

International audienc

Aqueous inorganic uranium speciation in European stream waters from the FOREGS dataset using geochemical modelling and determination of a U bioavailability baseline

International audienc

Investigation of the importance of site-specific fauna in environmental risk assessment for routine atmospheric radionuclidesreleases

International audienc