The Risk and Protective Factors for Adolescents with an Addicted Parent

This research project was a qualitative study that explored the risk and protective factors for adolescents who are growing up with a parent who is addicted to drugs and/or alcohol using a sample of eight participants. Specifically, this research looked at the possible protective factors that may help an adolescent from becoming addicted to drugs and/or alcohol themselves. Also, this research looked at the possible risk factors that may have helped an adolescent fall into addiction like his or her parents. In addition, the study explored possible behavioral patterns exhibited in adolescents who are growing up in a home with addiction and become addicted themselves. The themes that emerged from the data for risk factors for adolescents who grow up in a home with an addicted parent and who become addicted to drugs and/or alcohol themselves were a) parental chemical health modeling, b) traumatic experiences in adolescence and/or adulthood and c) genetic predisposition. In comparison, the themes that emerged for protective factors for adolescents who grow up in a home with an addicted parent and do not become addicted to drugs and/or alcohol were a) engagement with others and in activities, b) resilience and c) a faith in God. The predominant theme that emerged for the behavioral patterns of adolescents who grow up in an addicted home and become addicted to drugs and/or alcohol themselves was oppositional defiance. Overall, the data from this study had some similar findings as other research, but also provided new findings. It is imperative that clinicians continue to stay knowledgeable regarding the risk and protective factors for adolescents who are growing up in a home with parental addiction. Research showed that these factors are always changing and different for every adolescent. It is important for social workers to be aware of the diversity of these factors that could help or hinder an adolescent from following in their parent’s footsteps of addiction

The Risk and Protective Factors for Adolescents with an Addicted Parent

This research project was a qualitative study that explored the risk and protective factors for adolescents who are growing up with a parent who is addicted to drugs and/or alcohol using a sample of eight participants. Specifically, this research looked at the possible protective factors that may help an adolescent from becoming addicted to drugs and/or alcohol themselves. Also, this research looked at the possible risk factors that may have helped an adolescent fall into addiction like his or her parents. In addition, the study explored possible behavioral patterns exhibited in adolescents who are growing up in a home with addiction and become addicted themselves. The themes that emerged from the data for risk factors for adolescents who grow up in a home with an addicted parent and who become addicted to drugs and/or alcohol themselves were a) parental chemical health modeling, b) traumatic experiences in adolescence and/or adulthood and c) genetic predisposition. In comparison, the themes that emerged for protective factors for adolescents who grow up in a home with an addicted parent and do not become addicted to drugs and/or alcohol were a) engagement with others and in activities, b) resilience and c) a faith in God. The predominant theme that emerged for the behavioral patterns of adolescents who grow up in an addicted home and become addicted to drugs and/or alcohol themselves was oppositional defiance. Overall, the data from this study had some similar findings as other research, but also provided new findings. It is imperative that clinicians continue to stay knowledgeable regarding the risk and protective factors for adolescents who are growing up in a home with parental addiction. Research showed that these factors are always changing and different for every adolescent. It is important for social workers to be aware of the diversity of these factors that could help or hinder an adolescent from following in their parent’s footsteps of addiction

Emergence of novel methicillin resistant Staphylococcus pseudintermedius lineages revealed by whole genome sequencing of isolates from companion animals and humans in Scotland

Staphylococcus pseudintermedius is an opportunistic pathogen in dogs, and infection in humans is increasingly found, often linked to contact with dogs. We conducted a retrospective genotyping and antimicrobial susceptibility testing study of 406 S. pseudintermedius isolates cultured from animals (dogs, cats and an otter) and humans across Scotland, from 2007 to 2020. Seventy-five sequence types (STs) were identified, among the 130 isolates genotyped, with 59 seen only once. We observed the emergence of two methicillin resistant Staphylococcus pseudintermedius (MRSP) clones in Scotland: ST726, a novel locally-evolving clone, and ST551, first reported in 2015 in Poland, possibly linked to animal importation to Scotland from Central Europe. While ST71 was the most frequent S. pseudintermedius strain detected, other lineages that have been replacing ST71 in other countries, in addition to ST551, were detected. Multidrug resistance (MDR) was detected in 96.4% of MRSP and 8.4% of MSSP. A single MRSP isolate was resistant to mupirocin. Continuous surveillance for the emergence and dissemination of novel MDR MRSP in animals and humans and changes in antimicrobial susceptibility in S. pseudintermedius is warranted to minimise the threat to animal and human health

Emergence of novel methicillin resistant Staphylococcus pseudintermedius lineages revealed by whole genome sequencing of isolates from companion animals and humans in Scotland

Staphylococcus pseudintermedius is an opportunistic pathogen in dogs, and infection in humans is increasingly found, often linked to contact with dogs. We conducted a retrospective genotyping and antimicrobial susceptibility testing study of 406 S. pseudintermedius isolates cultured from animals (dogs, cats and an otter) and humans across Scotland, from 2007 to 2020. Seventy-five sequence types (STs) were identified, among the 130 isolates genotyped, with 59 seen only once. We observed the emergence of two methicillin resistant Staphylococcus pseudintermedius (MRSP) clones in Scotland: ST726, a novel locally-evolving clone, and ST551, first reported in 2015 in Poland, possibly linked to animal importation to Scotland from Central Europe. While ST71 was the most frequent S. pseudintermedius strain detected, other lineages that have been replacing ST71 in other countries, in addition to ST551, were detected. Multidrug resistance (MDR) was detected in 96.4% of MRSP and 8.4% of MSSP. A single MRSP isolate was resistant to mupirocin. Continuous surveillance for the emergence and dissemination of novel MDR MRSP in animals and humans and changes in antimicrobial susceptibility in S. pseudintermedius is warranted to minimise the threat to animal and human health

The spatial organization and microbial community structure of an epilithic biofilm.

Microbial biofilms are common on lithic surfaces, including stone buildings. However, the ecology of these communities is poorly understood. Few studies have focused on the spatial characteristics of lithobiontic biofilms, despite the fact that spatial structure has been demonstrated to influence ecosystem function (and hence biodegradation) and community diversity. Furthermore, relatively few studies have utilized molecular techniques to characterize these communities, even though molecular methods have revealed unexpected microbial diversity in other habitats. This study investigated (1) the spatial structure and (2) the taxonomic composition of an epilithic biofilm using molecular techniques, namely amplicon pyrosequencing and terminal restriction fragment length polymorphism. Dispersion indices and Mantel correlograms were used to test for the presence of spatial structure in the biofilm. Diversity metrics and rank-abundance distributions (RADs) were also generated. The study revealed spatial structure on a centimetre scale in eukaryotic microbes (fungi and algae), but not the bacteria. Fungal and bacterial communities were highly diverse; algal communities much less so. The RADs were characterized by a distinctive 'hollow' (concave up) profile and long tails of rare taxa. These findings have implications for understanding the ecology of epilithic biofilms and the spatial heterogeneity of stone biodeterioration.This work was supported by the Engineering and Physical Sciences Research Council (grant no. EP/G011338/1).This is the author accepted manuscript. The final version is available from OUP at http://femsec.oxfordjournals.org/content/91/3/fiu027

Genome-wide profiling of human cap-independent translation-enhancing elements.

We report an in vitro selection strategy to identify RNA sequences that mediate cap-independent initiation of translation. This method entails mRNA display of trillions of genomic fragments, selection for initiation of translation and high-throughput deep sequencing. We identified >12,000 translation-enhancing elements (TEEs) in the human genome, generated a high-resolution map of human TEE-bearing regions (TBRs), and validated the function of a subset of sequences in vitro and in cultured cells

Profiling microbial communities in manganese remediation systems treating coal mine drainage

Author Posting. © American Society for Microbiology, 2015. This article is posted here by permission of American Society for Microbiology for personal use, not for redistribution. The definitive version was published in Applied and Environmental Microbiology 81 (2015): 2189-2198, doi:10.1128/AEM.03643-14.Water discharging from abandoned coal mines can contain extremely high manganese levels. Removing this metal is an ongoing challenge. Passive Mn(II) removal beds (MRBs) contain microorganisms that oxidize soluble Mn(II) to insoluble Mn(III/IV) minerals, but system performance is unpredictable. Using amplicon pyrosequencing, we profiled the bacterial, fungal, algal and archaeal communities in four variably-performing MRBs in Pennsylvania to determine whether they differed among MRBs and from surrounding soil, and to establish the relative abundance of known Mn(II)-oxidizers. Archaea were not detected; PCRs with archaeal primers returned only non-target bacterial sequences. Fungal taxonomic profiles differed starkly between sites that remove the majority of influent Mn and those that do not, with the former dominated by Ascomycota (mostly Dothideomycetes) and the latter by Basidiomycota (almost entirely Agaricomycetes). Taxonomic profiles for the other groups did not differ significantly between MRBs, but OTU-based analyses showed significant clustering by MRB with all four groups (p<0.05). Soil samples clustered separately from MRBs in all groups except fungi, whose soil samples clustered loosely with their respective MRB. Known Mn(II) oxidizers accounted for a minor proportion of bacterial sequences (up to 0.20%) but a greater proportion of fungal sequences (up to 14.78%). MRB communities are more diverse than previously thought, and more organisms may be capable of Mn(II) oxidation than are currently known.This project was funded by Smithsonian Scholarly Studies and Next-Generation Sequencing grants to C.M.S., by a Smithsonian Postdoctoral Fellowship to D.L.C., and by the National Science Foundation, grant numbers EAR-1249489 (awarded to C.M.H.) and CBET-1336496 (awarded to C.M.H. and C.M.S.)

Les comportements patrimoniaux des ménages en France : évolutions et déterminants entre 2004 et 2010.

À la lumière des informations issues des enquêtes Patrimoine 2004 et 2010 de l’INSEE et des évaluations des comptes nationaux financiers établis par la Banque de France, l’article propose une première analyse des ajustements patrimoniaux des ménages français depuis 2007.épargne, ménages, portefeuilles, immobilier, risques.

Non-monotonic field-dependence of the ZFC magnetization peak in some systems of magnetic nanoparticles

We have performed magnetic measurements on a diluted system of gamma-Fe2O3 nanoparticles (~7nm), and on a ferritin sample. In both cases, the ZFC-peak presents a non-monotonic field dependence, as has already been reported in some experiments,and discussed as a possible evidence of resonant tunneling. Within simple assumptions, we derive expressions for the magnetization obtained in the usual ZFC, FC, TRM procedures. We point out that the ZFC-peak position is extremely sensitive to the width of the particle size distribution, and give some numerical estimates of this effect. We propose to combine the FC magnetization with a modified TRM measurement, a procedure which allows a more direct access to the barrier distribution in a field. The typical barrier values which are obtained with this method show a monotonic decrease for increasing fields, as expected from the simple effect of anisotropy barrier lowering, in contrast with the ZFC results. From our measurements on gamma-Fe2O3 particles, we show that the width of the effective barrier distribution is slightly increasing with the field, an effect which is sufficient for causing the observed initial increase of the ZFC-peak temperatures.Comment: LaTeX file 19 pages, 9 postscript figures. To appear in Phys. Rev. B (tentative schedule: Dec.97

Giant alkali-metal-induced lattice relaxation as the driving force of the insulating phase of alkali-metal/Si(111):B

Ab initio density-functional theory calculations, photoemission spectroscopy (PES), scanning tunneling microscopy, and spectroscopy (STM, STS) have been used to solve the 2√3 x 2√3R30 surface reconstruction observed previously by LEED on 0.5 ML K/Si:B. A large K-induced vertical lattice relaxation occurring only for 3/4 of Si adatoms is shown to quantitatively explain both the chemical shift of 1.14 eV and the ratio 1/3 measured on the two distinct B 1s core levels. A gap is observed between valence and conduction surface bands by ARPES and STS which is shown to have mainly a Si-B character. Finally, the calculated STM images agree with our experimental results. This work solves the controversy about the origin of the insulating ground state of alkali-metal/Si(111):B semiconducting interfaces which were believed previously to be related to many-body effectsThis work has received the financial support of the French ANR SURMOTT program (ANR-09-BLAN- 0210-01) and the Spanish MICIIN under Project No. FIS2010-1604