The effects of a plant proteinase inhibitor from Enterolobium contortisiliquum on human tumor cell lines

Supplementary to the efficient inhibition of trypsin, chymotrypsin, plasma kallikrein, and plasmin already described by the EcTI inhibitor from Enterolobium contortisiliquum, it also blocks human neutrophil elastase (K(iapp)=4.3 nM) and prevents phorbol ester (PMA)-stimulated activation of matrix metalloproteinase (MMP)-2 probably via interference with membrane-type 1 (MT1)-MMP. Moreover, plasminogen-induced activation of proMMP-9 and processing of active MMP-2 was also inhibited. Furthermore, the effect of EcTI on the human cancer cell lines HCT116 and HT29 (colorectal), SkBr-3 and MCF-7 (breast), K562 and THP-1 (leukemia), as well as on human primary fibroblasts and human mesenchymal stem cells (hMSCs) was studied. EcTI inhibited in a concentration range of 1.0-2.5 mu M rather specifically tumor cell viability without targeting primary fibroblasts and hMSCs. Taken together, our data indicate that the polyspecific proteinase inhibitor EcTI prevents proMMP activation and is cytotoxic against tumor cells without affecting normal tissue remodeling fibroblasts or regenerative hMSCs being an important tool in the studies of tumor cell development and dissemination

Stimulation of baculovirus transcriptome expression in mammalian cells by baculoviral transcriptional activators

Autographa californica multiple n ucleopolyhedro virus (AcMNPV), the type species of the family Baculoviridae, is an insect-specific virus that can enter a variety of mammalian cells. The potential of this versatile virus for protein expression or gene therapy in mammalian cells has become the focus of many studies. In most mammalian cells, transduced AcMNPV genes are either not expressed or expressed at an extremely low level. Here, we studied the effects of the two major AcMNPV trans-activators, IE1 and IE2, on the activation of AcMNPV genome in Vero E6 cells. Microarray analysis showed that when IE1 was overexpressed, it significantly activated genes gp64 and pe38, and upregulated ie2, he65, pcna, orf16, orf17 and orf25. Although, there were only two genes, pe38 and orf 17, that were activated by IE2, we discovered interestingly that the combination of IE1 and IE2 factors had a synergistic effect on activation of the AcMNPV genome in mammalian cells, and activated around 38%, or 59 out of the 155 genes placed on the microarray. This is the first detailed study of baculoviral transcription regulation in mammalian cells, and it shows that the baculoviral genome can be activated in a mammalian system, and also that the two major trans-activators, IE1 and IE2, play a central role in this activation

Glueball spectrum based on a rigorous three-dimensional relativistic equation for two-gluon bound states II: calculation of the glueball spectrum

In the preceding paper, a rigorous three-dimensional relativistic equation for two-gluon bound states was derived from the QCD with massive gluons and represented in the angular momentum representation. In order to apply this equation to calculate the glueball spectrum, in this paper, the equation is recast in an equivalent three-dimensional relativistic equation satisfied by the two-gluon positive energy state amplitude. The interaction Hamiltonian in the equation is exactly derived and expressed as a perturbative series. The first term in the series describes the one-gluon exchange interaction which includes fully the retardation effect in it. This term plus the linear confining potential are chosen to be the interaction Hamiltonian and employed in the practical calculation. With the integrals containing three and four spherical Bessel functions in the QCD vertices being analytically calculated, the interaction Hamiltonian is given an explicit expression in the angular momentum representation. Numerically solving the relativistic equation with taking the contributions arising from the retardation effect and the longitudinal mode of gluon fields into account, a set of masses for the $0^{++},0^{-+},1^{++},1^{-+},2^{++}$ and $2^{-+\text{}}$ glueball states are obtained and are in fairly good agreement with the predictions given by the lattice simulatio

Spontaneous CP Violation in a SUSY Model with a complex CKM

It is pointed out that the recent measurement of the angle $\gamma$ of the unitarity triangle, providing irrefutable evidence for a complex Cabibbo-Kobayashi-Maskawa (CKM) matrix, presents a great challenge for supersymmetric models with spontaneous CP violation. We construct a new minimal extension of the minimal supersymmetric standard model (MSSM), with spontaneous CP breaking, which leads to a complex CKM matrix, thus conforming to present experimental data. This is achieved through the introduction of two singlet chiral superfields and a vector-like quark chiral superfield which mixes with the standard quarks. A $Z_3$ symmetry is introduced in order to have a potential solution to the strong CP problem.Comment: 12 pages. Uses the elsart.cls LaTeX class. Minor corrections and references adde

Mixed metal node effect in zeolitic imidazolate frameworks

We synthesized two series of bimetallic (zinc and cobalt) zeolitic imidazolate frameworks (ZIF-62) under different solvothermal conditions. It is found that the structure of the derived ZIF crystals is highly sensitive to synthesis conditions. One series possesses the standard ZIF-62 structure, whereas the other has a mixed structure composed of both the standard structure and an unknown one. The standard series exhibits a slight negative deviation from linearity of melting temperature (T(m)) and glass transition temperature (T(g)) with the substitution of Co for Zn. In contrast, the new series displays a stronger negative deviation. These negative deviations from linearity indicate the mixed metal node effect in bimetallic ZIF-62 due to the structural mismatch between Co(2+) and Zn(2+) and to the difference in their electronic configurations. The new series involves both cobalt-rich and zinc-rich phases, whereas the standard one shows one homogeneous phase. Density functional theory calculations predict that the substitution of Co for Zn increases the bulk modulus of the ZIF crystals. This work indicates that the structure, melting behaviour, and mechanical properties of ZIFs can be tuned by metal node substitution and by varying the synthetic conditions. Both series of ZIFs have higher glass forming abilities due to their higher T(g)/T(m) ratios (0.77–0.84) compared to most good glass formers

Delocalization and spin-wave dynamics in ferromagnetic chains with long-range correlated random exchange

We study the one-dimensional quantum Heisenberg ferromagnet with exchange couplings exhibiting long-range correlated disorder with power spectrum proportional to $1/k^{\alpha}$, where $k$ is the wave-vector of the modulations on the random coupling landscape. By using renormalization group, integration of the equations of motion and exact diagonalization, we compute the spin-wave localization length and the mean-square displacement of the wave-packet. We find that, associated with the emergence of extended spin-waves in the low-energy region for $\alpha > 1$, the wave-packet mean-square displacement changes from a long-time super-diffusive behavior for $\alpha <1$ to a long-time ballistic behavior for $\alpha > 1$. At the vicinity of $\alpha =1$, the mobility edge separating the extended and localized phases is shown to scale with the degree of correlation as $E_c\propto (\alpha -1)^{1/3}$.Comment: PRB to appea

Quark initiated coherent diffractive production of muon pair and W boson at hadron colliders

The large transverse momentum muon pair and W boson productions in the quark initiated coherent diffractive processes at hadron colliders are discussed under the framework of the two-gluon exchange parametrization of the Pomeron model. In this approach, the production cross sections are related to the small-x off-diagonal gluon distribution and the large-x quark distribution in the proton (antiproton). By approximating the off-diagonal gluon distribution by the usual gluon distribution function, we estimate the production rates of these processes at the Fermilab Tevatron.Comment: 11pages, 6 PS figures, to appear in PR

An Extended Technicolor Model

An extended technicolor model is constructed. Quark and lepton masses, spontaneous CP violation, and precision electroweak measurements are discussed. Dynamical symmetry breaking is analyzed using the concept of the BIG MAC.Comment: 35 pages, Latex, YCTP-P21-93, BUHEP-93-2

Diffractive light quark jet production at hadron colliders in the two-gluon exchange model

Massless quark and antiquark jet production at large transverse momentum in the coherent diffractive processes at hadron colliders is calculated in the two-gluon exchange parametrization of the Pomeron model. We use the helicity amplitude method to calculate the cross section formula. We find that for the light quark jet production the diffractive process is related to the differential off-diagonal gluon distribution function in the proton. We estimate the production rate for this process at the Fermilab Tevatron by approximating the off-diagonal gluon distribution function by the usual diagonal gluon distribution in the proton. And we find that the cross sections for the diffractive light quark jet production and the charm quark jet production are in the same order of magnitude. We also use the helicity amplitude method to calculate the diffractive charm jet production at hadron colliders, by which we reproduce the leading logarithmic approximation result of this process we previously calculated.Comment: 15 pages, 4 PS figures, Revte

A Phenomenological Analysis of Gluon Mass Effects in Inclusive Radiative Decays of the J/ψ\rm{J/\psi} and $\Upsilon

The shapes of the inclusive photon spectra in the processes \Jp \to \gamma X and \Up \to \gamma X have been analysed using all available experimental data. Relativistic, higher order QCD and gluon mass corrections were taken into account in the fitted functions. Only on including the gluon mass corrections, were consistent and acceptable fits obtained. Values of $0.721^{+0.016}_{-0.068}$ GeV and $1.18^{+0.09}_{-0.29}$ GeV were found for the effective gluon masses (corresponding to Born level diagrams) for the \Jp and \Up respectively. The width ratios \Gamma(V \to {\rm hadrons})/\Gamma(V \to \gamma+ {\rm hadrons}) V=\Jp, \Up were used to determine $\alpha_s(1.5 {\rm GeV})$ and $\alpha_s(4.9 {\rm GeV})$. Values consistent with the current world average $\alpha_s$ were obtained only when gluon mass correction factors, calculated using the fitted values of the effective gluon mass, were applied. A gluon mass $\simeq 1$ GeV, as suggested with these results, is consistent with previous analytical theoretical calculations and independent phenomenological estimates, as well as with a recent, more accurate, lattice calculation of the gluon propagator in the infra-red region.Comment: 50 pages, 11 figures, 15 table