Description of a new species of Forcipomyia (Euprojoannisia) Brèthes, 1914 (Diptera: Ceratopogonidae) and a key to Chinese species of the subgenus

Forcipomyia (Euprojoannisia) coronacella Han et Hou sp. n. (Diptera: Ceratopogonidae) is described and illustrated based on male specimens from China. The new species is compared with the similar congeners, F. (Euprojoannisia) palustris (Meigen, 1804) and F. (Euprojoannisia) mucronis Liu et Yu, 2001. We provide separate keys for identification of the males and females of the species of the subgenus F. (Euprojoannisia) Brèthes in China

Generating Handwritten Chinese Characters using CycleGAN

Handwriting of Chinese has long been an important skill in East Asia. However, automatic generation of handwritten Chinese characters poses a great challenge due to the large number of characters. Various machine learning techniques have been used to recognize Chinese characters, but few works have studied the handwritten Chinese character generation problem, especially with unpaired training data. In this work, we formulate the Chinese handwritten character generation as a problem that learns a mapping from an existing printed font to a personalized handwritten style. We further propose DenseNet CycleGAN to generate Chinese handwritten characters. Our method is applied not only to commonly used Chinese characters but also to calligraphy work with aesthetic values. Furthermore, we propose content accuracy and style discrepancy as the evaluation metrics to assess the quality of the handwritten characters generated. We then use our proposed metrics to evaluate the generated characters from CASIA dataset as well as our newly introduced Lanting calligraphy dataset.Comment: Accepted at WACV 201

GPUを用いた高解像度画像の実時間ステレオマッチングシステムの研究

筑波大学 (University of Tsukuba)201

GPUを用いた高解像度画像の実時間ステレオマッチングシステムの研究

筑波大学 (University of Tsukuba)201

The research progress of metabonomics in acupuncture field

本文综合分析了国内外关于代谢组学技术运用于针灸机理研究的相关文献，发现目前代谢组学方法已经广泛应用于针灸研究，但是代谢组学研究结果难以全面阐释针灸作用机理，研究结果分析局限于代谢模式改变的分析，缺乏大样本临床试验数据支撑，对针刺、艾灸治疗手段的始动机制研究甚少。This paper aimed to analyze the relevant literature on mechanism studies of metabonomics in acupuncture field. The results showed that currently metabolomics has been widely used in acupuncture research, but metabolomics studies are difficult to fully explain the mechanism of acupuncture, the analysis is limited at the changes of metabolic patterns, large scaled clinical trials were few, researches that focused on how acupuncture and moxibustion started were poor

Genetically Modified Dermal Keratinocytes Express High Levels of Transforming Growth Factor-β1

In an attempt to genetically modify cultured keratinocytes with transforming growth factor-β1 (TGF-β1), which has been proven to be one of the most important cytokines involved in wound healing, two constructs were made. One, designated pG3Z:K14-TGF-β1, is a plasmid in which the expression of TGF-β1 is driven by the keratin 14 promoter. The other, designated pLin-TGF-β1, is a retroviral vector in which the retroviral 5' long-terminal repeat promoter drives expression. In both constructs, the deletion of a small fragment of the noncoding region of the TGF-β1 gene was made to differentiate the transcript from that for endogenously expressed TGF-β1. Different types of cells were transfected with the pG3Z:K14-TGF-β1 construct using the calcium phosphate method. The pLin-TGF-β1 construct was propagated in a retroviral packaging cell line and conditioned medium that contained high titers of the virus was used to transduce keratinocytes or other types of cells grown in standard culture. Northern analysis, used to evaluate the expression of TGF-β1 mRNA in the pG3Z:K14-TGF-β1 transfected keratinocyte Cl-177 cell line, showed a smaller TGF-β1 transcript compared with that endogenously expressed by dermal fibroblasts. The level of TGF-β1 protein evaluated by enzyme-linked immunosorbent assay was significantly higher in medium conditioned by either the K14-TGF-β1 transfected or the pLin-TGF-β1 transduced keratinocytes, compared with that obtained from control cells; however, the level of TGF-β1 protein was unchanged in cultures of pG3Z:K14-TGF-β1 transfected nonkeratinocyte cells such as fetal and adult fibroblasts. Using the mink lung epithelial cell growth inhibition assay, we found an increase in TGF-β1 activity in conditioned medium from the pG3Z:K14-TGF-β1 transfected cells. To evaluate possible paracrine effects of the keratinocyte derived TGF-β1, a coculture system was established with pLin-TGF-β1 transduced keratinocytes grown in the upper chamber and dermal fibroblasts in the lower chamber. The results showed that TGF-β1 released from keratinocytes diffused to the lower chamber where it stimulated collagen production by dermal fibroblasts. In summary, we demonstrate here that primary cultured keratinocytes can be genetically modified to express high levels of TGF-β1 and suggest that this offers a potential approach for the therapy of dermal lesions such as nonhealing wounds