Development of synchronous VHL syndrome tumors reveals contingencies and constraints to tumor evolution

This is an Open Access article distributed under the terms of the Creative Commons Attribution License.-- et al.[Background]: Genomic analysis of multi-focal renal cell carcinomas from an individual with a germline VHL mutation offers a unique opportunity to study tumor evolution. [Results]: We perform whole exome sequencing on four clear cell renal cell carcinomas removed from both kidneys of a patient with a germline VHL mutation. We report that tumors arising in this context are clonally independent and harbour distinct secondary events exemplified by loss of chromosome 3p, despite an identical genetic background and tissue microenvironment. We propose that divergent mutational and copy number anomalies are contingent upon the nature of 3p loss of heterozygosity occurring early in tumorigenesis. However, despite distinct 3p events, genomic, proteomic and immunohistochemical analyses reveal evidence for convergence upon the PI3K-AKT-mTOR signaling pathway. Four germline tumors in this young patient, and in a second, older patient with VHL syndrome demonstrate minimal intra-tumor heterogeneity and mutational burden, and evaluable tumors appear to follow a linear evolutionary route, compared to tumors from patients with sporadic clear cell renal cell carcinoma. [Conclusions]: In tumors developing from a germline VHL mutation, the evolutionary principles of contingency and convergence in tumor development are complementary. In this small set of patients with early stage VHL-associated tumors, there is reduced mutation burden and limited evidence of intra-tumor heterogeneity.RF and JL received funding from EU FP7 (PREDICT project), EB is a Rosetrees Trust fellow, NM received funding from the Rosetrees Trust, MG is funded by the UK Medical Research Council, IV is funded by Spanish Ministerio de Economía y Competitividad subprograma Ramón y Cajal, and CS is a senior Cancer Research UK clinical research fellow and is funded by Cancer Research UK, the Rosetrees Trust, EU FP7 (projects PREDICT and RESPONSIFY, ID:259303), the Prostate Cancer Foundation, and the Breast Cancer Research Foundation. This study was supported by researchers at the National Institute for Health Research Biomedical Research Centres at University College London Hospitals and at the Royal Marsden Hospital.Peer Reviewe

Conditions for Implementing Innovating Telemedicine Procedures After Hip Arthroplasty

Telemedicine technology offers a wide range of possibilities for improving various forms of healthcare services. These technologies can support and economize healthcare service processes including medical consultations, preparation for medical treatment and in the post-operative period. The increasing number of older people in developed countries increases the importance of telemedicine as a way to economize the care of the elderly. Domestic healthcare is one such opportunity. This study focuses on the use of this technology as a networking achievement demanding integrated relationships and purposeful interaction to implement and use this technology including focus on use of domestic healthcare of elderly patients after total hip arthroplasty in Poland. This network of actors consists of importantly interaction between patients, healthcare service providers and technology providers. A combination of telematics technology, services theory and contingency theory creates a distinct network approach to understanding domestic healthcare as a particular form of service process provision implying development of the exchange economy to support the services production economy. The aim of the article is to analyze the conditions for innovative telemedicine procedures implementing after total hip arthroplasty. Due to the conducted considerations, a set of fundamental solutions in regards to network implementation and application in home healthcare of Polish elderly patients after total hip arthroplasty will be developed. Conclusions will also concern the potential market of telemedicine solutions for patients after hip arthroplasty in Poland. Attention will also be paid to restrictions on the implementation of new technologies and ways to overcome them