High-Dose Nitrate Supplementation Attenuates the Increased Blood Pressure Responses to Isometric Blood Flow Restriction Exercise in Healthy Males

The effect of nitrate (NO3−) supplementation on blood pressure (BP) responses during large muscle mass isometric and ischaemic exercise in healthy young adults is unclear. The aim of the present study was to assess the effect of 5-day supplementation of NO3− on BP responses during a short isometric contraction and a sustained ischaemic contraction. In a randomised, double-blinded, crossover design, 14 healthy active young adults underwent BP measurements after 5 days of either NO3− (NIT) or placebo (PLA) supplementation. Beat-by-beat BP was measured at pre- and post-exercise rest, and during a short (20 s) isometric contraction at 25% maximal strength and throughout a sustained ischaemic contraction. Plasma nitrite (NO2−) concentration increased significantly after NO3− supplementation compared to placebo (475 ± 93 nmol·L−1 vs. 198 ± 46 nmol·L−1, p 0.05). Five days of NO3− supplementation elevated plasma NO2− concentration and reduced BP during a short isometric contraction and a sustained ischaemic contraction in healthy adults. These observations indicate that multiple-day nitrate supplementation can decrease BP at rest and attenuate the increased BP response during isometric exercise. These findings support that NO3− supplementation is an effective nutritional intervention in reducing SBP and MAP in healthy young males during submaximal exercise

Molecular phylogeny of diplomonads and enteromonads based on SSU rRNA, alpha-tubulin and HSP90 genes: Implications for the evolutionary history of the double karyomastigont of diplomonads

<p>Abstract</p> <p>Background</p> <p>Fornicata is a relatively recently established group of protists that includes the diplokaryotic diplomonads (which have two similar nuclei per cell), and the monokaryotic enteromonads, retortamonads and <it>Carpediemonas</it>, with the more typical one nucleus per cell. The monophyly of the group was confirmed by molecular phylogenetic studies, but neither the internal phylogeny nor its position on the eukaryotic tree has been clearly resolved.</p> <p>Results</p> <p>Here we have introduced data for three genes (SSU rRNA, α-tubulin and HSP90) with a wide taxonomic sampling of Fornicata, including ten isolates of enteromonads, representing the genera <it>Trimitus </it>and <it>Enteromonas</it>, and a new undescribed enteromonad genus. The diplomonad sequences formed two main clades in individual gene and combined gene analyses, with <it>Giardia </it>(and <it>Octomitus</it>) on one side of the basal divergence and <it>Spironucleus</it>, <it>Hexamita </it>and <it>Trepomonas </it>on the other. Contrary to earlier evolutionary scenarios, none of the studied enteromonads appeared basal to diplokaryotic diplomonads. Instead, the enteromonad isolates were all robustly situated within the second of the two diplomonad clades. Furthermore, our analyses suggested that enteromonads do not constitute a monophyletic group, and enteromonad monophyly was statistically rejected in 'approximately unbiased' tests of the combined gene data.</p> <p>Conclusion</p> <p>We suggest that all higher taxa intended to unite multiple enteromonad genera be abandoned, that <it>Trimitus </it>and <it>Enteromonas </it>be considered as part of Hexamitinae, and that the term 'enteromonads' be used in a strictly utilitarian sense. Our result suggests either that the diplokaryotic condition characteristic of diplomonads arose several times independently, or that the monokaryotic cell of enteromonads originated several times independently by secondary reduction from the diplokaryotic state. Both scenarios are evolutionarily complex. More comparative data on the similarity of the genomes of the two nuclei of diplomonads will be necessary to resolve which evolutionary scenario is more probable.</p

Practical Nutrition Strategies to Support Basketball Performance during International Short-Term Tournaments: A Narrative Review

A short-term (e.g., 6 days) basketball tournament is a shorter version of international tournaments, and qualification in it enables participation in international tournaments such as the Olympics and World championships or preparation before major tournaments. Time for recovery between matches is shorter compared with major tournaments, resulting in an accentuated load on players, which can be repeated up to four times within the 6-day competition period. Therefore, nutritional strategies need to focus on faster and adequate recovery after each match as well as optimum fuelling and hydration before and during matches. Travelling can also create additional challenges when preparing and/or applying those nutritional strategies. There are some particular evidence-based sport foods and ergogenic aids that can improve intermittent activity and/or the execution of motor skills, which may facilitate basketball players’ recovery and performance. The present review provides practical nutritional strategies to support short-term basketball tournaments based on players’ physiological needs and current sport nutrition guidelines

Relationship between Unilateral Leg Extension Strength and Dynamic Balance in Healthy Young Men

It is well known that the quadriceps muscle group is involved in activity during dynamic balance and that dynamic balance tests are an important feedback tool for predicting lower limb injuries. However, the relationship between maximum leg extension strength and performance in the Y Balance Test is not fully known. The aim of this study was to investigate the relationship between unilateral leg extension strength and dynamic balance in healthy young men. The study was conducted as a cross-sectional study. A total of 33 healthy men (mean age ± standard deviation = 21.21 ± 1.24 years) volunteered for this study. The participants’ dynamic balance was determined with the Y Balance Test and unilateral one repetition maximum strength was determined by the leg extension machine. The same side was preferred for strength and dynamic balance measurements. Normalized reach, composite score, and absolute and relative strength values were analyzed for correlations. Linear regression analysis was used to determine whether strength values predicted the results of the Y Balance Test. There was a positive linear correlation between the strength values and normalized reach distances and composite scores (r ranges from 0.466 to 0.757; p < 0.01). The coefficients of determination showed that dynamic balance and strength (absolute and relative) performance are not independent parameters in healthy young men (r2 = 21–57% explained variance). It was also found that strength values (absolute and relative) can predict balance. Our study confirmed the relationship between strength and dynamic balance. It can be said that especially the strength of the lower extremity has an acceptable effect on dynamic balance

Does Dominant Somatotype Differentiate Performance of Jumping and Sprinting Variables in Young Healthy Adults?

The relationship between an athlete’s somatotype three-numeral rating and his or her athletic performance is well known. However, a direct effect of the different dominant somatotype on jumping and sprinting variables has not yet been reported. The aim of this study was to investigate the effects of dominant somatotype on sport-specific explosive variables. One hundred and twelve physically active young adults (mean ± standard deviation age: 21.82 ± 3.18 years) were somatotype-rated using the Heath–Carter method. Participants were classified as balanced ectomorph, balanced mesomorph, central, mesomorph-endomorph, and mesomorphic ectomorph. Vertical jump and linear sprint tests were performed to measure peak lower body performance and sprint variables (time, speed, and momentum), respectively. The analysis revealed that balanced mesomorph had significantly higher vertical jump (effect size (ES) = 1.10, p = 0.005) and power to body mass (ES = 1.04, p = 0.023) than mesomorph-endomorph. In addition, balanced mesomorph showed significantly superior performance in 30-m sprint time and velocity than central and mesomorph-endomorph (ES range = 0.93–1, p < 0.05). Finally, balanced ectomorph (ES = 1.12, p = 0.009) and mesomorphic ectomorph (ES = 1.10, p = 0.017) were lower in sprint momentum compared to balanced mesomorphs. In conclusion, this study has shown the importance of the interaction between subtypes and athletic performance. The knowledge gained may be important in identifying those who tend to perform well in sports with explosive power and in prescribing training programs

Emerging Infectious Disease leads to Rapid Population Decline of Common British Birds

Emerging infectious diseases are increasingly cited as threats to wildlife, livestock and humans alike. They can threaten geographically isolated or critically endangered wildlife populations; however, relatively few studies have clearly demonstrated the extent to which emerging diseases can impact populations of common wildlife species. Here, we report the impact of an emerging protozoal disease on British populations of greenfinch Carduelis chloris and chaffinch Fringilla coelebs, two of the most common birds in Britain. Morphological and molecular analyses showed this to be due to Trichomonas gallinae. Trichomonosis emerged as a novel fatal disease of finches in Britain in 2005 and rapidly became epidemic within greenfinch, and to a lesser extent chaffinch, populations in 2006. By 2007, breeding populations of greenfinches and chaffinches in the geographic region of highest disease incidence had decreased by 35% and 21% respectively, representing mortality in excess of half a million birds. In contrast, declines were less pronounced or absent in these species in regions where the disease was found in intermediate or low incidence. Also, populations of dunnock Prunella modularis, which similarly feeds in gardens, but in which T. gallinae was rarely recorded, did not decline. This is the first trichomonosis epidemic reported in the scientific literature to negatively impact populations of free-ranging non-columbiform species, and such levels of mortality and decline due to an emerging infectious disease are unprecedented in British wild bird populations. This disease emergence event demonstrates the potential for a protozoan parasite to jump avian host taxonomic groups with dramatic effect over a short time period

Athletic performance, sports experience, and exercise addiction: an association study on ANKK1 gene polymorphism rs1800497

IntroductionExercise addiction is a phenomenon being able to affecting the athletic performance. The gene, ANKK1 and the polymorphism NM_178510.2:c.2137G &gt; A (rs1800497) has been linked to the exercise addiction. However, further studies on diverse populations and sport branches are needed to totally explore the possible association of this polymorphism with the athletic performance. Thus, the present study aims to decipher any possible relations of the rs1800497 polymorphism with the athletic performance/personal best (PB) and sport experience of elite athletes.MethodsSixty volunteer elite athletes (31 sprint/power and 29 endurance) and 20 control/sedentary participated in the study. The polymorphism was genotyped using whole exome sequencing approach and PB were determined according to the International Association of Athletics Federations (IAAF) score.ResultsOur results underlined that there were not any significance differences for both allele and genotype frequencies between the groups in terms of athletic performance, although the frequency of allele G was higher (p &gt; 0.05). Nevertheless, sport experience significantly associated with the rs1800496 polymorphism (p &lt; 0.05).DiscussionIn conclusion, genotype G/G could be inferred to be linked to the higher sport experience and athletic performance. Still, further studies with higher number of participants are needed to conclude the association of this polymorphism with athletic parameters

Molecular Phylogeny and Evolution of Parabasalia with Improved Taxon Sampling and New Protein Markers of Actin and Elongation Factor-1α

BACKGROUND: Inferring the evolutionary history of phylogenetically isolated, deep-branching groups of taxa-in particular determining the root-is often extraordinarily difficult because their close relatives are unavailable as suitable outgroups. One of these taxonomic groups is the phylum Parabasalia, which comprises morphologically diverse species of flagellated protists of ecological, medical, and evolutionary significance. Indeed, previous molecular phylogenetic analyses of members of this phylum have yielded conflicting and possibly erroneous inferences. Furthermore, many species of Parabasalia are symbionts in the gut of termites and cockroaches or parasites and therefore formidably difficult to cultivate, rendering available data insufficient. Increasing the numbers of examined taxa and informative characters (e.g., genes) is likely to produce more reliable inferences. PRINCIPAL FINDINGS: Actin and elongation factor-1α genes were identified newly from 22 species of termite-gut symbionts through careful manipulations and seven cultured species, which covered major lineages of Parabasalia. Their protein sequences were concatenated and analyzed with sequences of previously and newly identified glyceraldehyde-3-phosphate dehydrogenase and the small-subunit rRNA gene. This concatenated dataset provided more robust phylogenetic relationships among major groups of Parabasalia and a more plausible new root position than those previously reported. CONCLUSIONS/SIGNIFICANCE: We conclude that increasing the number of sampled taxa as well as the addition of new sequences greatly improves the accuracy and robustness of the phylogenetic inference. A morphologically simple cell is likely the ancient form in Parabasalia as opposed to a cell with elaborate flagellar and cytoskeletal structures, which was defined as most basal in previous inferences. Nevertheless, the evolution of Parabasalia is complex owing to several independent multiplication and simplification events in these structures. Therefore, systematics based solely on morphology does not reflect the evolutionary history of parabasalids

Association of ACTN3 R577X Polymorphism with Elite Basketball Player Status and Training Responses

The α-actinin-3 (ACTN3) gene rs1815739 (C/T, R577X) polymorphism is a variant frequently associated with athletic performance among different populations. However, there is limited research on the impact of this variant on athlete status and physical performance in basketball players. Therefore, the aim of this study was twofold: (1) to determine the association of ACTN3 rs1815739 polymorphism with changes in physical performance in response to six weeks of training in elite basketball players using 30 m sprint and Yo-Yo Intermittent Recovery Test Level 2 (IR 2) tests, and (2) to compare ACTN3 genotype and allelic frequencies between elite basketball players and controls. The study included a total of 363 individuals, comprising 101 elite basketball players and 262 sedentary individuals. Genomic DNA was isolated from oral epithelial cells or leukocytes, and genotyping was performed by real-time PCR using KASP genotyping method or by microarray analysis. We found that the frequency of the ACTN3 rs1815739 XX genotype was significantly lower in basketball players compared to controls (10.9 vs. 21.4%, p = 0.023), suggesting that RR/RX genotypes were more favorable for playing basketball. Statistically significant (p = 0.045) changes were observed in Yo-Yo IRT 2 performance measurement tests in basketball players with the RR genotype only. In conclusion, our findings suggest that the carriage of the ACTN3 rs1815739 R allele may confer an advantage in basketball