Effects of Uniaxial Cyclic Strain on Endothelial Progenitor Cells

Despite the high prevalence of calcific aortic valve disease (CAVD), the underlying mechanisms of pathogenesis have not been found yet. Therefore, it is extremely important to study CAVD and understand how it develops. For this matter, we decided to study the potential of endothelial progenitor cells (EPCs) for use in tissue-engineered models of heart valves. EPCs were chosen as the cell source of interest for this study due to their high neovascularization potential and use in regenerative medicine and cardiovascular tissue engineering. In this project, we aimed to engineer the microenvironment of cells that are involved in the formation of heart valves. We hypothesized that cyclic strain induces EPCs to undergo differentiation, which will depend on the applied strain, culture media components and culture duration. EPCs isolated from human umbilical cord blood expressed endothelial cell markers CD31 and vascular endothelial growth factor receptor 2 (VEGFR2), and the progenitor cell marker CD34. The cells did not express the mesenchymal marker α-smooth muscle actin (α-SMA). EPCs showed an endothelial behavior demonstrated by the uptake of acetylated-low density lipoprotein (Dil-Ac-LDL), and a vasculogenic potential demonstrated by tube formation. The cells were subjected to 20% strain rates by utilizing a cyclic uniaxial biostretcher for 7 days and showed a mild expression of α-SMA. Considering these events, EPCs were subjected to 20% strain for longer periods of time (1, 2, and 3 weeks) and showed maintained CD31 expression, no α-SMA expression, and increased CD34 expression suggesting an increased vascular-like behavior after strain

5D Rotating Black Holes and the nAdS2_2/nCFT1_1 Correspondence

We study rotating black holes in five dimensions using the nAdS$_2$/nCFT$_1$ correspondence. A consistent truncation of pure Einstein gravity (with a cosmological constant) in five dimensions to two dimensions gives a generalization of the Jackiw-Teitelboim theory that has two scalar fields: a dilaton and a squashing parameter that breaks spherical symmetry. The interplay between these two scalar fields is non trivial and leads to interesting new features. We study the holographic description of this theory and apply the results to the thermodynamics of the rotating black hole from a two dimensional point of view. This setup challenges notions of universality that have been advanced based on simpler models: we find that the mass gap of Kerr-AdS$_5$ corresponds to an undetermined effective coupling in the nAdS$_2$/nCFT$_1$ theory which depends on ultraviolet data.Comment: 49 pages; v2 minor comments and references added; v3 fixed minor typos in eqs. (4.5) and (4.26

The Spectrum of Static Subtracted Geometries

Subtracted geometries are black hole solutions of the four dimensional STU model with rather interesting ties to asymptotically flat black holes. A peculiar feature is that the solutions to the Klein-Gordon equation on this subtracted background can be organized according to representations of the conformal group $SO(2,2)$. We test if this behavior persists for the linearized fluctuations of gravitational and matter fields on static, electrically charged backgrounds of this kind. We find that there is a subsector of the modes that do display conformal symmetry, while some modes do not. We also discuss two different effective actions that describe these subtracted geometries and how the spectrum of quasinormal modes is dramatically different depending upon the action used.Comment: 30 pages, 2 figures. v2: references added. v3: minor corrections to match with published versio

Geodesic Diagrams, Gravitational Interactions & OPE Structures

We give a systematic procedure to evaluate conformal partial waves involving symmetric tensors for an arbitrary CFT$_d$ using geodesic Witten diagrams in AdS$_{d+1}$. Using this procedure we discuss how to draw a line between the tensor structures in the CFT and cubic interactions in AdS. We contrast this map to known results using three-point Witten diagrams: the maps obtained via volume versus geodesic integrals differ. Despite these differences, we show how to decompose four-point exchange Witten diagrams in terms of geodesic diagrams, and we discuss the product expansion of local bulk fields in AdS.Comment: typos corrected, references adde