Assessing speech intelligibility level of children with phonological disorders: implications in social skills

OBJETIVO: analisar a relação entre o grau de inteligibilidade de fala e as habilidades sociais de comunicação em crianças com diagnóstico de desvio fonológico. MÉTODO:participaram deste estudo 10 crianças, diagnosticadas com desvio fonológico, com idade cronológica média de 7,28 anos, sendo três do gênero feminino e sete do masculino, que realizavam terapia de linguagem duas vezes por semana em uma clínica escola de uma cidade de médio porte do estado de São Paulo. As crianças foram submetidas à avaliação da fonologia por meio do instrumento ABFW, sendo que a gravidade do desvio fonológico foi baseada no cálculo do Percentual de Consoantes Corretas (PCC). E em seguida foram filmadas em situações estruturas de interação de maneira tal a obter uma amostra de fala espontânea, para caracterizar o grau de inteligibilidade de fala (GIF), além de observar presença ou ausência dos componentes das habilidades sociais de comunicação (HSC). Para analisar a relação entre GIF e HSC foi utilizado o coeficiente de Spearman. RESULTADOS:foi possível caracterizar as classes de HSC que encontram-se deficitárias em crianças diagnosticas com desvio fonológico, além de verificar uma relação significante e positiva entre o GIF e o HSC, mostrando que quanto maior o GIF maior a dificuldades nas HSC. CONCLUSÃO: o estudo verificou a relação entre GIF e HSC, além de identificar as classes de HSC que encontram-se deficitárias em crianças com diagnóstico de desvio fonológico, favorecendo a elaboração de instrumentos para avaliação e intervenção, permitindo assim, um melhor planejamento para cada caso.PURPOSE: to analyze the relationship between the degree of speech intelligibility and social communication skills in children diagnosed with phonological disorders. METHOD: the study included 10 children diagnosed with phonological disorders, with an average chronological age of 7.28 years and three females and seven males, performing speech-language therapy twice a week in a nursing school in a medium-sized town in the state of São Paulo. The children were assessed by the phonology of the ABFW instrument, and the severity of phonological disorder was based on the Percentage of Consonants Correct (PCC). And then they were video-typed in interaction structures situations in such a way as to obtain a sample of spontaneous speech, in order to characterize the degree of speech intelligibility (GIF), in addition to observing the presence or absence of the components of social communication skills (HSC). In order to examine the relationship between GIF and HSC we used the Spearman coefficient. RESULTS: it was possible to characterize HSC classes that are deficient in children with phonological diagnostic, and to check a significant and positive relationship between GIF and HSC, showing that the higher GIF is the greater are the difficulties in HSC. CONCLUSION: this study examined the relationship between GIF and HSC, and identified classes of HSC that are deficient in children diagnosed with phonological disorders, promoting the development of instruments for assessment and intervention, thus allowing better planning for each case

Assessment of receptive and expressive auditory and visual functions in pre-term children

TEMA: prematuridade como fator de risco para atraso no desenvolvimento da linguagem. OBJETIVO: verificar o desempenho de crianças prematuras quanto às áreas auditiva receptiva, expressiva e visual. MÉTODO: participaram da amostra 40 crianças de idade cronológica entre 12 e 24 meses. O grupo experimental (G1) foi composto por 20 crianças que apresentaram em seu histórico de vida os fatores de risco prematuridade e baixo peso ou muito baixo peso. A idade gestacional das crianças variou de 22 a 34, semanas todas com peso abaixo de 2500g; este grupo foi dividido em função do peso, ou seja, crianças de baixo peso e de muito baixo peso. O grupo controle (G2) foi composto por 20 crianças nascidas a termo com peso superior a 2500g, sem histórico para atraso do desenvolvimento. Os procedimentos constaram de entrevista com os pais e aplicação da Escala Early Language Milestone Scale (ELM). RESULTADOS: na comparação entre grupos, os resultados mostraram ser estatisticamente significativos. As crianças do G1 apresentaram prejuízo na área auditiva expressiva, auditiva receptiva e visual, embora algumas crianças tivessem apresentado resultados esperados para sua faixa etária, em alguma das funções avaliadas. A área mais prejudicada foi a área expressiva. CONCLUSÃO: as crianças do G1 apresentaram alteração nas áreas auditiva receptiva, auditiva expressiva e visual. As crianças prematuras com muito baixo peso apresentaram maiores prejuízos nas áreas avaliadas.BACKGROUND: prematurity as a risk factor for delay in language development. AIM: to verify the performance of premature children regarding their receptive and expressive auditory and visual abilities. METHOD: participants were 40 children with chronological ages between 12 and 24 months. The experimental group (G1) was composed by 20 children who presented report of prematurity and low or very low weight. The birth age varied from 22 to 34 weeks and weight was below 2500gr. This group was divided according to weight, i.e. children with low and very low weight. The control group (G2) was composed by 20 children born at term, with weight above 2500gr and with no report of development delay. The procedures consisted of an interview with the parents and the application of the Early Language Milestone Scale - ELM. RESULTS: when comparing the groups, the results indicate statistically significant differences. Children of G1 presented a poorer performance in the Expressive Hearing (EH), Receptive Hearing (RH) and Visual (V) areas, although a few children of this group presented the expected results for their age group in some of the tested abilities. The expressive auditory ability was the most affected. CONCLUSION: children of G1 presented deficits in the expressive and receptive auditory and visual functions. Premature children with very low weight presented higher deficits in the tested abilities

Latin American registry of renal involvement in COVID-19 disease. The relevance of assessing proteinuria throughout the clinical course

The Latin American Society of Nephrology and Hypertension conducted a prospective cohort, multinational registry of Latin American patients with kidney impairment associated to COVID-19 infection with the objective to describe the characteristics of acute kidney disease under these circumstances. The study was carried out through open invitation in order to describe the characteristics of the disease in the region. Eight-hundred and seventy patients from 12 countries were included. Median age was 63 years (54–74), most of patients were male (68.4%) and with diverse comorbidities (87.2%). Acute kidney injury (AKI) was hospital-acquired in 64.7% and non-oliguric in 59.9%. Multiorgan dysfunction syndrome (MODS) due to COVID-19 and volume depletion were the main factors contributing to AKI (59.2% and 35.7% respectively). Kidney replacement therapy was started in 46.2%. Non-recovery of renal function was observed in 65.3%. 71.5% of patients were admitted to ICU and 72.2% underwent mechanical ventilation. Proteinuria at admission was present in 62.4% of patients and proteinuria during hospital-stay occurred in 37.5%. Those patients with proteinuria at admission had higher burden of comorbidities, higher baseline sCr, and MODS was severe. On the other hand, patients with de novo proteinuria had lower incidence of comorbidities and near normal sCr at admission, but showed adverse course of disease. COVID-19 MODS was the main cause of AKI in both groups. All-cause mortality of the general population was 57.4%, and it was associated to age, sepsis as cause of AKI, severity of condition at admission, oliguria, mechanical ventilation, non-recovery of renal function, in-hospital complications and hospital stay. In conclusion, our study contributes to a better knowledge of this condition and highlights the relevance of the detection of proteinuria throughout the clinical course

Identification of Phosphoproteins as Possible Differentiation Markers in All-Trans-Retinoic Acid-Treated Neuroblastoma Cells

BACKGROUND: Neuroblastic tumors account for 9-10% of pediatric tumors and neuroblastoma (NB) is the first cause of death in pre-school age children. NB is classified in four stages, depending on the extent of spreading. A fifth type of NB, so-called stage 4S (S for special), includes patients with metastatic tumors but with an overall survival that approximates 75% at five years. In most of these cases, the tumor regresses spontaneously and regression is probably associated with delayed neuroblast cell differentiation. METHODOLOGY/PRINCIPAL FINDINGS: In order to identify new early markers to follow and predict this process for diagnostic and therapeutics intents, we mimicked the differentiation process treating NB cell line SJ-NK-P with all-trans-retinoic acid (ATRA) at different times; therefore the cell proteomic pattern by mass spectrometry and the phosphoproteomic pattern by a 2-DE approach coupled with anti-phosphoserine and anti-phosphotyrosine western blotting were studied. CONCLUSIONS/SIGNIFICANCE: Proteomic analysis identified only two proteins whose expression was significantly different in treated cells versus control cells: nucleoside diphosphate kinase A (NDKA) and reticulocalbin-1 (RCN1), which were both downregulated after 9 days of ATRA treatment. However, phosphoproteomic analysis identified 8 proteins that were differentially serine-phosphorylated and 3 that were differentially tyrosine-phosphorylated after ATRA treatment. All proteins were significantly regulated (at least 0.5-fold down-regulated). Our results suggest that differentially phosphorylated proteins could be considered as more promising markers of differentiation for NB than differentially expressed proteins

Search for dark matter produced in association with bottom or top quarks in √s = 13 TeV pp collisions with the ATLAS detector

A search for weakly interacting massive particle dark matter produced in association with bottom or top quarks is presented. Final states containing third-generation quarks and miss- ing transverse momentum are considered. The analysis uses 36.1 fb−1 of proton–proton collision data recorded by the ATLAS experiment at √s = 13 TeV in 2015 and 2016. No significant excess of events above the estimated backgrounds is observed. The results are in- terpreted in the framework of simplified models of spin-0 dark-matter mediators. For colour- neutral spin-0 mediators produced in association with top quarks and decaying into a pair of dark-matter particles, mediator masses below 50 GeV are excluded assuming a dark-matter candidate mass of 1 GeV and unitary couplings. For scalar and pseudoscalar mediators produced in association with bottom quarks, the search sets limits on the production cross- section of 300 times the predicted rate for mediators with masses between 10 and 50 GeV and assuming a dark-matter mass of 1 GeV and unitary coupling. Constraints on colour- charged scalar simplified models are also presented. Assuming a dark-matter particle mass of 35 GeV, mediator particles with mass below 1.1 TeV are excluded for couplings yielding a dark-matter relic density consistent with measurements

Aprendizajes y prácticas educativas en las actuales condiciones de época: COVID-19

“Esta obra colectiva es el resultado de una convocatoria a docentes, investigadores y profesionales del campo pedagógico a visibilizar procesos investigativos y prácticas educativas situadas en el marco de COVI-19. La misma se inscribe en el trabajo llevado a cabo por el equipo de Investigación responsable del Proyecto “Sentidos y significados acerca de aprender en las actuales condiciones de época: un estudio con docentes y estudiantes de la educación secundarias en la ciudad de Córdoba” de la Facultad de Filosofía y Humanidades. Universidad Nacional de Córdoba. El momento excepcional que estamos atravesando, pero que también nos atraviesa, ha modificado la percepción temporal a punto tal que habitamos un tiempo acelerado y angustiante que nos exige la producción de conocimiento provisorio. La presente publicación surge como un espacio para detenernos a documentar lo que nos acontece y, a su vez, como oportunidad para atesorar y resguardar las experiencias educativas que hemos construido, inventado y reinventando en este contexto. En ella encontrarán pluralidad de voces acerca de enseñar y aprender durante la pandemia. Este texto es una pausa para reflexionar sobre el hacer y las prácticas educativas por venir”.Fil: Beltramino, Lucia (comp.). Universidad Nacional de Córdoba. Facultad de Filosofía y Humanidades. Escuela de Archivología; Argentina

31st Annual Meeting and Associated Programs of the Society for Immunotherapy of Cancer (SITC 2016) : part two

Background The immunological escape of tumors represents one of the main ob- stacles to the treatment of malignancies. The blockade of PD-1 or CTLA-4 receptors represented a milestone in the history of immunotherapy. However, immune checkpoint inhibitors seem to be effective in specific cohorts of patients. It has been proposed that their efficacy relies on the presence of an immunological response. Thus, we hypothesized that disruption of the PD-L1/PD-1 axis would synergize with our oncolytic vaccine platform PeptiCRAd. Methods We used murine B16OVA in vivo tumor models and flow cytometry analysis to investigate the immunological background. Results First, we found that high-burden B16OVA tumors were refractory to combination immunotherapy. However, with a more aggressive schedule, tumors with a lower burden were more susceptible to the combination of PeptiCRAd and PD-L1 blockade. The therapy signifi- cantly increased the median survival of mice (Fig. 7). Interestingly, the reduced growth of contralaterally injected B16F10 cells sug- gested the presence of a long lasting immunological memory also against non-targeted antigens. Concerning the functional state of tumor infiltrating lymphocytes (TILs), we found that all the immune therapies would enhance the percentage of activated (PD-1pos TIM- 3neg) T lymphocytes and reduce the amount of exhausted (PD-1pos TIM-3pos) cells compared to placebo. As expected, we found that PeptiCRAd monotherapy could increase the number of antigen spe- cific CD8+ T cells compared to other treatments. However, only the combination with PD-L1 blockade could significantly increase the ra- tio between activated and exhausted pentamer positive cells (p= 0.0058), suggesting that by disrupting the PD-1/PD-L1 axis we could decrease the amount of dysfunctional antigen specific T cells. We ob- served that the anatomical location deeply influenced the state of CD4+ and CD8+ T lymphocytes. In fact, TIM-3 expression was in- creased by 2 fold on TILs compared to splenic and lymphoid T cells. In the CD8+ compartment, the expression of PD-1 on the surface seemed to be restricted to the tumor micro-environment, while CD4 + T cells had a high expression of PD-1 also in lymphoid organs. Interestingly, we found that the levels of PD-1 were significantly higher on CD8+ T cells than on CD4+ T cells into the tumor micro- environment (p < 0.0001). Conclusions In conclusion, we demonstrated that the efficacy of immune check- point inhibitors might be strongly enhanced by their combination with cancer vaccines. PeptiCRAd was able to increase the number of antigen-specific T cells and PD-L1 blockade prevented their exhaus- tion, resulting in long-lasting immunological memory and increased median survival

Adding 6 months of androgen deprivation therapy to postoperative radiotherapy for prostate cancer: a comparison of short-course versus no androgen deprivation therapy in the RADICALS-HD randomised controlled trial

Background Previous evidence indicates that adjuvant, short-course androgen deprivation therapy (ADT) improves metastasis-free survival when given with primary radiotherapy for intermediate-risk and high-risk localised prostate cancer. However, the value of ADT with postoperative radiotherapy after radical prostatectomy is unclear. Methods RADICALS-HD was an international randomised controlled trial to test the efficacy of ADT used in combination with postoperative radiotherapy for prostate cancer. Key eligibility criteria were indication for radiotherapy after radical prostatectomy for prostate cancer, prostate-specific antigen less than 5 ng/mL, absence of metastatic disease, and written consent. Participants were randomly assigned (1:1) to radiotherapy alone (no ADT) or radiotherapy with 6 months of ADT (short-course ADT), using monthly subcutaneous gonadotropin-releasing hormone analogue injections, daily oral bicalutamide monotherapy 150 mg, or monthly subcutaneous degarelix. Randomisation was done centrally through minimisation with a random element, stratified by Gleason score, positive margins, radiotherapy timing, planned radiotherapy schedule, and planned type of ADT, in a computerised system. The allocated treatment was not masked. The primary outcome measure was metastasis-free survival, defined as distant metastasis arising from prostate cancer or death from any cause. Standard survival analysis methods were used, accounting for randomisation stratification factors. The trial had 80% power with two-sided α of 5% to detect an absolute increase in 10-year metastasis-free survival from 80% to 86% (hazard ratio [HR] 0·67). Analyses followed the intention-to-treat principle. The trial is registered with the ISRCTN registry, ISRCTN40814031, and ClinicalTrials.gov, NCT00541047. Findings Between Nov 22, 2007, and June 29, 2015, 1480 patients (median age 66 years [IQR 61–69]) were randomly assigned to receive no ADT (n=737) or short-course ADT (n=743) in addition to postoperative radiotherapy at 121 centres in Canada, Denmark, Ireland, and the UK. With a median follow-up of 9·0 years (IQR 7·1–10·1), metastasis-free survival events were reported for 268 participants (142 in the no ADT group and 126 in the short-course ADT group; HR 0·886 [95% CI 0·688–1·140], p=0·35). 10-year metastasis-free survival was 79·2% (95% CI 75·4–82·5) in the no ADT group and 80·4% (76·6–83·6) in the short-course ADT group. Toxicity of grade 3 or higher was reported for 121 (17%) of 737 participants in the no ADT group and 100 (14%) of 743 in the short-course ADT group (p=0·15), with no treatment-related deaths. Interpretation Metastatic disease is uncommon following postoperative bed radiotherapy after radical prostatectomy. Adding 6 months of ADT to this radiotherapy did not improve metastasis-free survival compared with no ADT. These findings do not support the use of short-course ADT with postoperative radiotherapy in this patient population