Extruded linseed and linseed oil as alternative to soybean meal and soybean oil in diets for fattening lambs

The study evaluated the effects of replacing soybean meal and soybean oil respectively with extruded linseed and linseed oil on the productive performances and meat quality traits in lambs slaughtered at 90 days. Lambs weaned at 40 days were divided into 3 groups (N.=10) fed ad libitum for 6 weeks as follows: C (control, commercial feed containing soybean meal and soybean oil); LO (feed containing linseed oil instead of soybean oil); EL (feed containing extruded linseed). Meat quality traits were evaluated on the Longissimus lumborum (Ll) and Semimembranosus (Sm) muscles. The lambs' growth performances and the slaughtering and sectioning data did not differ between groups. The redness of meat was significantly higher (P<0.05) for the LO and EL groups compared to the control for both the muscles tested. Ll meat samples of the EL group showed a greater cooking loss compared to LO (P<0.01) and to the control (P<0.05). The amount of linoleic acid in raw Ll meat samples was significantly (P<0.01) lower in both LO and EL groups with respect to control. The concentration of α-linolenic acid was significantly (P<0.01) higher in the EL group and this positively affected the total content of ω3 as well as the ω6/ω3 ratio

Sheep (Ovis aries) T cell receptor alpha (TRA) and delta (TRD) genes and genomic organization of the TRA/TRD locus

In mammals, T cells develop along two discrete pathways characterized by expression of either the αβ or the γδ T cell receptors. Human and mouse display a low peripheral blood γδ T cell percentage ("γδ low species") while sheep, bovine and pig accounts for a high proportion of γδ T lymphocytes ("γδ high species"). While the T cell receptor alpha (TRA) and delta (TRD) genes and the genomic organization of the TRA/TRD locus has been determined in human and mouse, this information is still poorly known in artiodactyl species, such as sheep

The expansion of the TRB and TRG genes in domestic goats (Capra hircus) is characteristic of the ruminant species

Goats (Capra hircus), one of the first domesticated species, are economically important for milk and meat production, and their broad geographical distribution reflects their successful adaptation to diverse environmental conditions. Despite the relevance of this species, the genetic research on the goat traits is limited compared to other domestic species. Thanks to the latest goat reference genomic sequence (ARS1), which is considered to be one of the most continuous assemblies in livestock, we deduced the genomic structure of the T cell receptor beta (TRB) and gamma (TRG) loci in this ruminant species

Effect of dietary rosemary oil on growth performance and flesh quality of farmed seabass (Dicentrarchus labrax)

In order to assess the effect of a diet integrated with rosemary oil on growth perfor- mance and flesh quality of farmed Dicentrarchus labrax, a feeding trial was carried out. Seabass were fed with two different diets for 200 days, after that they were caught and stored in ice in a refrigerator cell (0-2°C) for 17 days. Then they were examinated on 1, 3, 10 and 17 day for sensory evaluation (fre- sh whole fish and cooked fillet) according to QIM and Torry Scheme. Specific growth rate (SGR) and feed conversion rate (FCR) were calculated, fillets were subjected to proximate analysis. No statistical differences were found in the final average body weight for both diets as well as SGR and FCR means. The sensory score (QIM) increased linearly with storage time. No significant difference was noticed between two diets. As for cooked fillet, Torry Scheme score decreased with storage time. At day 10 significant differences were displayed between the diets (7.44 vs. 8.67; P<0.05). Overall sensory accep- tability for consuming farmed seabass stored in ice was set in corrispondence of day 13 for control diet and day 14 for experimental diet. No difference was showed in the chemical composition

Genomic and comparative analysis of the T cell receptor gamma locus in two Equus species

The genus Equus is the only extant genus of the Equidae family, which belongs to Perissodactyla, an order of mammals characterized by an odd number of toes (odd-toes ungulates). Taking advantage of the latest release of the genome assembly, we studied, for the first time in two organisms belonging to the Equus genus, the horse (Equus caballus) and the donkey (Equus asinus), the T cell receptor gamma (TRG) locus encoding the gamma chain of the γδ T cell receptor. Forty-five Variable (TRGV) genes belonging to the seven IMGT-NC validated mammalian TRGV subgroups, 25 Joining (TRGJ) and 17 Constant (TRGC) genes organized in 17 V-J-(J)-C cassettes, in tandem on about 1100 Kb, characterize the horse TRG locus, making the horse TRG locus the one with the greatest extension and with a significantly higher number of genes than the orthologous loci of the other mammalian species. A clonotype analysis of an RNA-seq transcriptomic dataset derived from spleen of an adult healthy horse, using the complete set of the horse TRGJ germline gene sequences as a probe, revealed that, in addition to the most prominent V-J rearrangements within each cassette, there is a relevant proportion of trans-cassette V-J recombination, whereby the same TRGV genes can recombine with different TRGJ genes spliced to the corresponding TRGC genes. This recombinant event strongly contributes to the diversity of the γ chain repertoire. In the donkey TRG locus, 34 TRGV, 21 TRGJ and 14 TRGC genes distributed in 14 V-J-(J)-C cassettes were found in a region of approximately 860 kb. Although the donkey’s TRG is smaller than that of the horse, in Equus genus, this is still the second largest locus so far found in any mammalian species. Finally, the comparative analysis highlighted differences in size and gene content between the horse and donkey TRG loci, despite belonging to the same genus, indicating a good level of diversification within Equus. These data is in agreement with the evolutionary idea of the existence of a Equus recent common ancestor in rapid evolution, for which a mutation rate between horses and donkeys is more comparable to that between species belonging to different genera rather than to species of the same genus

Chromosome instability in lymphocytes of Friesian cows naturally exposed to dioxins being raised close to a metallurgic factory area in southern Italy

Dioxins (PCDDs, PCDFs and DL-PCBs) are a large family of congeners that are considered highly toxic and are reported to be teratogenic, mutagenic, carcinogenic, immunotoxic and hepatotoxic, also affecting the nervous and reproductive systems. Farm animals are particularly exposed to these chemicals when they are fed with grass produced close to polluted areas such as those located in vicinity of metallurgic factories. Cytogenetic tests can be very useful to check genetic damage occurring to domestic animal cells exposed to these chemicals. Fifty-two randomly selected Italian Friesian cows (Bos taurus, 2n&nbsp;=&nbsp;60) from two farms located in the vicinity of and (as a control) far from the a metallurgic industrial area underwent cytogenetic investigations to ascertain possible differences in their chromosome fragility. One farm was under legal sequestration due to the presence in the milk mass of higher mean values of dioxins (24.78&nbsp;±&nbsp;3.19 pg&nbsp;g−1 of fat as sum of PCDD + PCDF + DL-PCBs as WHO-TEQ (World Health Organization-Toxic Equivalent Quantity), with DL-PCBs being the main chemical component) than those permitted (5.5 pg&nbsp;g−1 of fat as WHO-TEQ). Cytogenetic analyses, performed by using both the chromosome abnormality (CA) test (chromosome and chromatid breaks) and sister chromatid exchange (SCE) test, revealed a significantly (p&nbsp;&lt;&nbsp;0.01) higher chromosome fragility in cells of exposed cows (26 cows) compared to those of the control (23 cows)