Suivi rhéologique du processus d'agrégation de la protéine tau

International audienceAlzheimer's disease is one of thirty pathology called conformational disease that are characterized by errors folding and assembly of protein. In Alzheimer's disease, tau protein and amyloid beta peptide are responsible of the neuronal degeneration. Understanding these mechanisms and diagnosis are closely linked to the availability of an efficient analytical concept for monitoring ex vivo self-assembly of proteins. To understand the aggregation's mechanism, a microsystem is developed for the detection of protein tau based on the micro-rheological followed by high frequency ultrasonic waves. The sensor used is a TSM (Thickness Shear Mode) resonator operating in shear mode at a fundamental frequency of 5MHz. In contact with the fluid to be characterized, the sensor generate shear waves, which are measured in reflection through instrumentation developed in the laboratory. From the measurement of the complex impedance of quartz with a network analyzer, the extraction of G' (elastic modulus) and G'' (viscous modulus) is possible. Initial results indicate that our sensor can quantify, by viscosity, concentration variations of a peptide sequence of tau protein, and can differentiate, by plasticity, the conformational state.  </p

L'intrapreneuriat : une pédagogie innovante

International audienceL’objectif de cet article est de présenter une approche pédagogie basée sur l’intrapreneuriat qui est une approche transversale par l'acquisition de compétences métiers. Elle vise à émuler la créativité, des savoirs et savoir-faire au sein d’espaces innovants (FacLab, living Lab, …) et dans un contexte intrapreneurial. Ces compétences nécessitent que chacun des acteurs intègrent les contours et mécanismes des autres partenaires. Cette approche a été implémentée à l’IUT de Cergy-Pontoise (département GEII Sarcelles) et s’articule sur une période de 3 années incluant le DUT et la licence professionnelle IMNEOV. La finalité de ce parcours vise à former des techniciens supérieurs aux métiers du numérique. La méthode adoptée est basée sur une pédagogie active par projet où l’enseignant s’adapte au niveau de l’apprenant

Développement de capteurs et d'instrumentations pour champ proche acoustique

MONTPELLIER-BU Sciences (341722106) / SudocSudocFranceF

Suivi rhéologique du processus d'agrégation de la protéine tau

International audienceAlzheimer's disease is one of thirty pathology called conformational disease that are characterized by errors folding and assembly of protein. In Alzheimer's disease, tau protein and amyloid beta peptide are responsible of the neuronal degeneration. Understanding these mechanisms and diagnosis are closely linked to the availability of an efficient analytical concept for monitoring ex vivo self-assembly of proteins. To understand the aggregation's mechanism, a microsystem is developed for the detection of protein tau based on the micro-rheological followed by high frequency ultrasonic waves. The sensor used is a TSM (Thickness Shear Mode) resonator operating in shear mode at a fundamental frequency of 5MHz. In contact with the fluid to be characterized, the sensor generate shear waves, which are measured in reflection through instrumentation developed in the laboratory. From the measurement of the complex impedance of quartz with a network analyzer, the extraction of G' (elastic modulus) and G'' (viscous modulus) is possible. Initial results indicate that our sensor can quantify, by viscosity, concentration variations of a peptide sequence of tau protein, and can differentiate, by plasticity, the conformational state.  </p

Ultrasonic microrheology for ex vivo skin explants monitoring: A proof of concept

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Rheological monitoring for tau protein aggregation process

International audienceAlzheimer’s disease is one of thirty pathology called conformational disease which are characterized by errors folding and assembly of protein. In Alzheimer’s disease, tau protein and amyloid beta peptide are responsible of the neuronal degeneration. Understanding these mechanisms and diagnosis are closely linked to the availability of an efficient analytical concept for monitoring ex vivo self-assembly of proteins. To answer the aggregation’s mechanism, a microsystem is developed for the detection of protein tau based on the micro-rheological followed by high frequency ultrasonic waves. The sensor used is a TSM (Thickness Shear Mode) resonator operating in shear mode at a fundamental frequency of 5MHz. It is composed of a quartz substrate, where on each side of the quartz is manufactured a titanium/gold electrode. The electrodes are obtained by conventional microfabrication process, with deposits by sputtering, photolithography and dry and wet etching. In contact with the fluid to be characterized, the sensor generate shear waves, which are measured in reflection through instrumentation developed in the laboratory. From the measurement of the complex impedance of quartz with a network analyzer, the extraction of G’ (elastic modulus) and G’’ (viscous modulus) is possible. The first experiment is to detect different concentrations of one of the peptide sequence, which is necessary and sufficient for the self-assembly of the tau protein. For this purpose, concentrations of peptide between 30μM and 480μM are deposited on the surface of the sensor. The 3D conformation depends on the peptide concentration. At 30μM, the peptide is in core form, while at higher concentrations (above 180μM), it will fibrillated. Initial results indicate that our sensor can quantify, by viscosity, concentration variations of a peptide sequence of tau protein, and can differentiate, by plasticity, the conformational state

Monitoring of different complex materials with TSM resonators array

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Chirp-Z analysis for sol–gel transition monitoring

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Effects of materials conductivity on the viscosity measurement using a QCM

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Microrheological monitoring of life cycle of yeast cell Saccharomyces Cerevisiae

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