Trends in wintertime climate in the northeastern United States: 1965–2005

Humans experience climate variability and climate change primarily through changes in weather at local and regional scales. One of the most effective means to track these changes is through detailed analysis of meteorological data. In this work, monthly and seasonal trends in recent winter climate of the northeastern United States (NE-US) are documented. Snow cover and snowfall are important components of the region\u27s hydrological systems, ecosystems, infrastructure, travel safety, and winter tourism and recreation. Temperature, snowfall, and snow depth data were collected from the merged United States Historical Climate Network (USHCN) and National Climatic Data Center Cooperative Network (COOP) data set for the months of December through March, 1965–2005. Monthly and seasonal time series of snow-covered days (snow depth \u3e2.54 cm) are constructed from daily snow depth data. Spatial coherence analysis is used to address data quality issues with daily snowfall and snow depth data, and to remove stations with nonclimatic influences from the regional analysis. Monthly and seasonal trends in mean, minimum, and maximum temperature, total snowfall, and snow-covered days are evaluated over the period 1965–2005, a period during which global temperature records and regional indicators exhibit a shift to warmer climate conditions. NE-US regional winter mean, minimum, and maximum temperatures are all increasing at a rate ranging from 0.42° to 0.46°C/decade with the greatest warming in all three variables occurring in the coldest months of winter (January and February). The regional average reduction in number of snow-covered days in winter (−8.9 d/decade) is also greatest during the months of January and February. Further analysis with additional regional climate modeling is required to better investigate the causal link between the increases in temperature and reduction in snow cover during the coldest winter months of January and February. In addition, regionally averaged winter snowfall has decreased by about 4.6 cm/decade, with the greatest decreases in snowfall occurring in December and February. These results have important implications for the impacts of regional climate change on the northeastern United States hydrology, natural ecosystems, and economy

The Gristhorpe coffin and its contents

In July 1834 excavation of a barrow at Gristhorpe, near Scarborough, Yorkshire, recovered an intact, waterlogged, hollowed-out oak coffin containing a perfectly preserved Bronze Age skeleton that had been wrapped in an animal skin and buried with worked flints, a bronze dagger with a whalebone pommel, and a bark vessel apparently containing food residue. Gristhorpe Man became the centrepiece of the Scarborough Philosophical Society’s museum display. In 2004, planned refurbishment of the renamed Rotunda Museum provided the opportunity for a scientific re-examination of the burial and grave goods in order to eluciate the life and death of this extraordinary survival of the British Early Bronze Age. Tree-trunk coffin burials are relatively rare and Gristhorpe Man, with his range of grave goods was likely to have held a special role in society. Analysis of the skeleton included an examination of its skeletal morphology and palaeopathological conditions combined with isotopic analyses of the bones and teeth in order to investigate mobility, diet, and status of the individual whose unusual large stature, dentition, and novel methods of conservation were of particular interest. These analyses, combined with examination of the surviving coffin lid, including the unique ‘face’ carved onto one end of it, the grave goods, and radiocarbon and dendrochronological dating, reveal fascinating insights into the social position, inter-regional contacts and the burial rite associated with this enigmatic mature man who probably saw active combat and who suffered from a benign brain tumour that may have seriously altered his personality in his later years

Optimizing sequencing protocols for leaderboard metagenomics by combining long and short reads.

As metagenomic studies move to increasing numbers of samples, communities like the human gut may benefit more from the assembly of abundant microbes in many samples, rather than the exhaustive assembly of fewer samples. We term this approach leaderboard metagenome sequencing. To explore protocol optimization for leaderboard metagenomics in real samples, we introduce a benchmark of library prep and sequencing using internal references generated by synthetic long-read technology, allowing us to evaluate high-throughput library preparation methods against gold-standard reference genomes derived from the samples themselves. We introduce a low-cost protocol for high-throughput library preparation and sequencing

Planning for Sustainability in Small Municipalities: The Influence of Interest Groups, Growth Patterns, and Institutional Characteristics

How and why small municipalities promote sustainability through planning efforts is poorly understood. We analyzed ordinances in 451 Maine municipalities and tested theories of policy adoption using regression analysis.We found that smaller communities do adopt programs that contribute to sustainability relevant to their scale and context. In line with the political market theory, we found that municipalities with strong environmental interests, higher growth, and more formal governments were more likely to adopt these policies. Consideration of context and capacity in planning for sustainability will help planners better identify and benefit from collaboration, training, and outreach opportunities

Effect of promoter architecture on the cell-to-cell variability in gene expression

According to recent experimental evidence, the architecture of a promoter, defined as the number, strength and regulatory role of the operators that control the promoter, plays a major role in determining the level of cell-to-cell variability in gene expression. These quantitative experiments call for a corresponding modeling effort that addresses the question of how changes in promoter architecture affect noise in gene expression in a systematic rather than case-by-case fashion. In this article, we make such a systematic investigation, based on a simple microscopic model of gene regulation that incorporates stochastic effects. In particular, we show how operator strength and operator multiplicity affect this variability. We examine different modes of transcription factor binding to complex promoters (cooperative, independent, simultaneous) and how each of these affects the level of variability in transcription product from cell-to-cell. We propose that direct comparison between in vivo single-cell experiments and theoretical predictions for the moments of the probability distribution of mRNA number per cell can discriminate between different kinetic models of gene regulation.Comment: 35 pages, 6 figures, Submitte

Changes in Scottish suicide rates during the Second World War

BACKGROUND: It is believed that total reported suicide rates tend to decrease during wartime. However, analysis of suicide rates during recent conflicts suggests a more complex picture, with increases in some age groups and changes in method choice. As few age and gender specific analyses of more distant conflicts have been conducted, it is not clear if these findings reflect a change in the epidemiology of suicide in wartime. Therefore, we examined suicide rates in Scotland before, during and after the Second World War to see if similar features were present. METHODS: Data on deaths in Scotland recorded as suicide during the period 1931 – 1952, and population estimates for each of these years, were obtained from the General Register Office for Scotland. Using computer spreadsheets, suicide rates by gender, age and method were calculated. Forward stepwise logistic regression was used to assess the effect of gender, war and year on suicide rates using SAS V8.2. RESULTS: The all-age suicide rate among both men and women declined during the period studied. However, when this long-term decline is taken into account, the likelihood of suicide during the Second World War was higher than during both the pre-War and post-War periods. Suicide rates among men aged 15–24 years rose during the Second World War, peaking at 148 per million (41 deaths) during 1942 before declining to 39 per million (10 deaths) by 1945, while the rate among men aged 25–34 years reached 199 per million (43 deaths) during 1943 before falling to 66 per million (23 deaths) by 1946. This was accompanied by an increase in male suicides attributable to firearms and explosives during the War years which decreased following its conclusion. CONCLUSION: All age male and female suicide rates decreased in Scotland during World War II. However, once the general background decrease in suicide rates over the whole period is accounted for, the likelihood of suicide among the entire Scottish population during the Second World War was elevated. The overall decrease in suicide rates concealed large increases in younger male age groups during the War years, and an increase in male suicides recorded as due to the use of firearms. We conclude that the effects of war on younger people, reported in recent conflicts in Central Europe, were also seen in Scotland during the Second World War. The results support the findings of studies of recent conflicts which have found a heterogeneous picture with respect to age specific suicide rates during wartime

Severe MUPS in a sick-listed population: a cross-sectional study on prevalence, recognition, psychiatric co-morbidity and impairment

Background: Medically unexplained physical symptoms (MUPS) have a high prevalence in the general population and are associated with psychiatric morbidity. There are indications that MUPS are an important determinant of frequent and long-term disability. The primary objective was to assess the prevalence of MUPS in sick-listed-employees and its associations with depressive disorders, anxiety disorders, health anxiety, distress and functional impairment. Secondary objectives were to investigate the classification of the occupational health physicians (OHPs), their opinions about the causes as well as the attributions of the employee. Methods: In a cross- sectional study of 489 sick-listed employees from 5 OHP group practices, MUPS, depressive disorders, anxiety disorders, health anxiety, distress and functional impairment were assessed with the Patient Health Questionnaire (PHQ), the Whitely Index (WI), the Four-Dimensional Symptom Questionnaire (4DSQ) and the Short-Form 36 Health Survey (SF-36). We used a cut off score of 15 on the PHQ for the categorisation of severe MUPS. The opinions of the OHPs were evaluated by means of a separate questionnaire with regard to the presence of employees physical symptoms, and the symptoms attributions, and the diagnoses of the OHPs. Results: Severe MUPS had a prevalence of 15.1% in this population of sick-listed employees. These employees had 4-6 times more depressive and anxiety disorders, and were more impaired. Female gender and PHQ-9 scores were determinants of severe MUPS. Most of the time the OHPs diagnosed employees with severe MUPS as having a mental disorder. The employees attributed their physical symptoms in 66% to mental or to both mental and physical causes. Conclusion: The prevalence of severe MUPS is higher in long-term sick-listed employees than in the non-sick-listed working population and at least equals the prevalence in the general practice population. Severe MUPS are associated with psychiatric morbidity and functional impairment and must therefore be specifically recognised as such. Validated questionnaires, such as the PHQ-15, are useful instruments in order to help OHPs to recognise severe MUPS