15 research outputs found
Testing for Network and Spatial Autocorrelation
Testing for dependence has been a well-established component of spatial
statistical analyses for decades. In particular, several popular test
statistics have desirable properties for testing for the presence of spatial
autocorrelation in continuous variables. In this paper we propose two
contributions to the literature on tests for autocorrelation. First, we propose
a new test for autocorrelation in categorical variables. While some methods
currently exist for assessing spatial autocorrelation in categorical variables,
the most popular method is unwieldy, somewhat ad hoc, and fails to provide
grounds for a single omnibus test. Second, we discuss the importance of testing
for autocorrelation in data sampled from the nodes of a network, motivated by
social network applications. We demonstrate that our proposed statistic for
categorical variables can both be used in the spatial and network setting
B-cell chronic lymphocytic leukemia cells express a surface membrane phenotype of activated, antigen-experienced B lymphocytes
Damle RN, Ghiotto F, Valetto A, et al. B-cell chronic lymphocytic leukemia cells express a surface membrane phenotype of activated, antigen-experienced B lymphocytes. BLOOD. 2002;99(11):4087-4093.B-cell chronic lymphocytic leukemia (B-CLL) is considered an accumulative disease of antigen-naive CD5(+) B lymphocytes that circulate in the resting state. However, to evaluate the possibility that B-CLL cells resemble antigen-experienced and activated B cells, we analyzed the expression of markers of cellular activation and differentiation on CD5(+)CD19(+) cells from B-CLL patients and from age-matched healthy donors. The leukemic cells from all B-CLL patients, including those that lack significant numbers of V gene mutations, bear the phenotype of activated B cells based on the overexpression of the activation markers CD23, CD25, CD69, and CD71 and the underexpression of CD22, Fcgamma receptor IIb, CD79b, and immunoglobulin D that are down-regulated by cell triggering and activation. Furthermore, these leukemic cells resemble antigen-experienced lymphocytes in the underexpression of molecules that are down-regulated by cell triggering and in the uniform expression of CD27, an identifier of memory B cells. A comparison of the phenotypes of B-CLL patients with and without immunoglobulin V gene mutations suggests that the 2 subgroups differ both in specific marker expression (CD69, CD71, CD62 L, CD40, CD39, and HLA-DR) and in the time since antigenic stimulation, based on the reciprocal relationship of CD69 and CD71 expression. These findings imply that the leukemic cells from all B-CLL cases (irrespective of V gene mutations) exhibit features of activated and of antigen-experienced B lymphocytes and that the B-CLL cells that differ in immunoglobulin V genotype may have different antigen-encounter histories. (C) 2002 by The American Society of Hematology