A green approach for the quantification of daptomycin in pharmaceutical formulation by UV spectrophotometry

abstract Daptomycin is the first approved drug from a new class of antimicrobials, the cyclic lipopeptides, and is a very important antimicrobial agent in current clinical practice. Currently, there are no "green" analytical methods described in the literature to analyze the typical pharmaceutical dosage form of daptomycin. Thus, the aim of this work was to validate an environment-friendly spectrophotometric method in the UV region, for the analysis of daptomycin as a lyophilized powder. Water was used as diluent and the analyses were carried out on a spectrophotometer at 221 nm. The method met all validation requirements of the ICH guidelines, over a concentration range of 6-21 µg mL-1. A Student's t-test demonstrated that the proposed method was comparable to an HPLC method previously validated. Thus, the validated spectrophotometric method could quantify daptomycin in a powder form for injectable solutions, while being an economical, rapid, and "green" alternative for routine analysis in quality control

Respostas morfológicas e produtivas do capim-marandu adubado com doses combinadas de nitrogênio e enxofre Morphological and productive responses of marandugrass to combined rates of nitrogen and sulphur

Avaliaram-se os efeitos de combinações de doses de nitrogênio com doses de enxofre nas respostas morfológicas e produtivas de Brachiaria brizantha cv. Marandu cultivada em solução nutritiva, utilizando-se sílica como substrato, em um experimento em casa de vegetação no período da primavera. Utilizou-se o esquema fatorial 5² fracionado, com 13 combinações para nitrogênio e enxofre na solução nutritiva, em mg L-1: 14 e 3,2; 14 e 32; 14 e 80; 126 e 12,8; 126 e 64; 210 e 3,2; 210 e 32; 210 e 80; 336 e 12,8; 336 e 64; 462 e 3,2; 462 e 32; e 462 e 80, as quais foram distribuídas segundo o delineamento estatístico de blocos ao acaso, com quatro repetições. As avaliações foram realizadas em dois estádios de crescimento das plantas. Os resultados demonstraram que o fornecimento de enxofre é fundamental quando se aplica o nitrogênio. Para a maximização do número de perfilhos e de folhas, da produção de massa seca de folhas, de colmos+bainhas e da parte aérea, da área foliar e da taxa de aparecimento de folhas do capim-marandu, a combinação deve ser, no mínimo, de 358 mg L-1 para nitrogênio com 56 mg L-1 de enxofre. A taxa máxima de aparecimento de folhas para o capim-marandu ocorreu na dose de enxofre de 42 mg L-1.<br>This experiment was carried out in a greenhouse during the spring to study combinations of nitrogen and sulphur rates on Brachiaria brizantha cv. Marandu growing in nutrient solution and using ground quartz as substrate. It was set in a fractionated 5² factorial arrangement, with five rates of nitrogen and five rates of sulphur. The resulting 13 combinations were, in mg L-1: 14 and 3.2, 14 and 32, 14 and 80, 126 and 12.8, 126 and 64, 210 and 3.2, 210 and 32, 210 and 80, 336 and 12.8, 336 and 64, 462 and 3.2, 462 and 32, and 462 and 80. The experimental units were set in randomized blocks design, with four replications. Plants had two growth periods. The results showed that high availability of sulphur is required when nitrogen is supplied for total tiller number, leaf number, leaf dry matter yield, stems plus sheaths and plant tops, leaf area and leaf appearance rate. In order to maximize these variables for the marandugrass, it is necessary the minimum combination of 358 mg L-1 of nitrogen with 56 mg L-1 of sulphur. The highest leaf appearance rate for marandugrass occurred in the sulphur rate of 42 mg L-1