Influência do treinamento excêntrico nas razões de torque de flexores/extensores do joelho

O treinamento excêntrico (Texc) produz adaptações musculares que minimizam a ocorrência de lesões e é usado em reabilitação e treinamento de força, mas pouco se sabe sobre seus efeitos no equilíbrio entre músculos antagonistas do joelho. As razões de torque permitem determinar esses desequilíbrios musculares. O objetivo do estudo foi avaliar os efeitos de 12 semanas de Texc nas razões de torque excêntrico (Iexc:Qexc) entre os músculos isquiotibiais (I) e quadríceps (Q). Vinte e quatro sujeitos saudáveis do sexo masculino foram distribuídos nos grupos controle (GC, n=13, idade 27,7±4,6 anos) e experimental (GE, n=11, idade 28,5±9,5 anos), submetido ao treinamento. Um dinamômetro isocinético foi utilizado para o Texc (velocidade de -60 º/s) e para as avaliações (uma a cada quatro semanas). As razões de torque medidas foram comparadas estatisticamente entre os grupos e intragrupos entre as avaliações, com nível de significância de 5%. No GE, foi observada redução das razões de torque da avaliação (AV) inicial para as demais: AV1x AV2, p=0,005; AV1x AV3, p=0,001; e AV1x AV4, pEccentric training produces skeletal muscle adaptations that help preventing muscle injuries, being often used in rehabilitation and physical fitness programs, but little is known of the effects of this training in the balance between knee antagonistic muscles. Torque ratios allow determining such balance. The purpose of this study was to assess the effect of a 12-week eccentric training program on the eccentric torque ratio between hamstring and quadriceps muscles (Hecc:Qecc). Twenty-four healthy male subjects were assigned to either a control group (CG, n=13, aged 27.7±4.6 years) or an experimental group (EG, n=11, aged 28.5±9.5 years). An isokinetic dinamometer was used (angular velocity -60º/s) for both the eccentric training and the assessments, performed every four weeks. Torque ratios measured were statistically compared between groups and intragroups between assessments, with (significance level set at p<0.05. In EG a reduction in torque ratios was found from the initial assessment (AS1) to the other three ones: AS1x AS2, p=0.005; AS1x AS3, p=0.001; and AS1x AS4, p<0.001. At the last evaluation, EG torque ratios were lower than those of CG's (p=0.041). Eccentric training hence changes balance between knee flexor and extensor muscles: a 12-week training program leads to lower Hecc:Qecc ratio and to extensor torque increase, with no significant change in flexor torque, being thus suitable for rehabilitation aimed at strengthening knee extensor muscles

Genetic variation and exercise-induced muscle damage: implications for athletic performance, injury and ageing.

Prolonged unaccustomed exercise involving muscle lengthening (eccentric) actions can result in ultrastructural muscle disruption, impaired excitation-contraction coupling, inflammation and muscle protein degradation. This process is associated with delayed onset muscle soreness and is referred to as exercise-induced muscle damage. Although a certain amount of muscle damage may be necessary for adaptation to occur, excessive damage or inadequate recovery from exercise-induced muscle damage can increase injury risk, particularly in older individuals, who experience more damage and require longer to recover from muscle damaging exercise than younger adults. Furthermore, it is apparent that inter-individual variation exists in the response to exercise-induced muscle damage, and there is evidence that genetic variability may play a key role. Although this area of research is in its infancy, certain gene variations, or polymorphisms have been associated with exercise-induced muscle damage (i.e. individuals with certain genotypes experience greater muscle damage, and require longer recovery, following strenuous exercise). These polymorphisms include ACTN3 (R577X, rs1815739), TNF (-308 G>A, rs1800629), IL6 (-174 G>C, rs1800795), and IGF2 (ApaI, 17200 G>A, rs680). Knowing how someone is likely to respond to a particular type of exercise could help coaches/practitioners individualise the exercise training of their athletes/patients, thus maximising recovery and adaptation, while reducing overload-associated injury risk. The purpose of this review is to provide a critical analysis of the literature concerning gene polymorphisms associated with exercise-induced muscle damage, both in young and older individuals, and to highlight the potential mechanisms underpinning these associations, thus providing a better understanding of exercise-induced muscle damage

Iron Behaving Badly: Inappropriate Iron Chelation as a Major Contributor to the Aetiology of Vascular and Other Progressive Inflammatory and Degenerative Diseases

The production of peroxide and superoxide is an inevitable consequence of aerobic metabolism, and while these particular "reactive oxygen species" (ROSs) can exhibit a number of biological effects, they are not of themselves excessively reactive and thus they are not especially damaging at physiological concentrations. However, their reactions with poorly liganded iron species can lead to the catalytic production of the very reactive and dangerous hydroxyl radical, which is exceptionally damaging, and a major cause of chronic inflammation. We review the considerable and wide-ranging evidence for the involvement of this combination of (su)peroxide and poorly liganded iron in a large number of physiological and indeed pathological processes and inflammatory disorders, especially those involving the progressive degradation of cellular and organismal performance. These diseases share a great many similarities and thus might be considered to have a common cause (i.e. iron-catalysed free radical and especially hydroxyl radical generation). The studies reviewed include those focused on a series of cardiovascular, metabolic and neurological diseases, where iron can be found at the sites of plaques and lesions, as well as studies showing the significance of iron to aging and longevity. The effective chelation of iron by natural or synthetic ligands is thus of major physiological (and potentially therapeutic) importance. As systems properties, we need to recognise that physiological observables have multiple molecular causes, and studying them in isolation leads to inconsistent patterns of apparent causality when it is the simultaneous combination of multiple factors that is responsible. This explains, for instance, the decidedly mixed effects of antioxidants that have been observed, etc...Comment: 159 pages, including 9 Figs and 2184 reference

Probing Electron Transfer in the Manganese-Oxide-Forming MnxEFG Protein Complex using Fourier Transformed AC Voltammetry: Understanding the Oxidative Priming Effect

MnxG, a multicopper oxidase, is an enzyme from the marine Bacillus species, which produces manganese oxide minerals through the aerobic oxidation of dissolved Mn − a key process in global manganese geochemical cycling. When isolated in an active form as a part of the MnxEFG protein complex, the enzymatic activity of MnxG is substantially enhanced by mild oxidative priming. Herein, the mechanism for this effect is probed by using direct current (dc) and Fourier transformed alternating current (ac) voltammetric analysis of the MnxEFG complex and the catalytically inactive MnxEF subunit immobilised on a carbon electrode. Analysis of these ac voltammetric data reveals a significant enhancement in the rate of electron transfer in the Type 2 Cu sites upon oxidative priming of the enzyme, which is attributed to the improved catalytic activity of MnxG in the MnxEFG protein complex. 2