Plasmon-Assisted Delivery of Single Nano-Objects in an Optical Hot Spot

Fully exploiting the capability of nano-optics to enhance light-matter interaction on the nanoscale is conditioned by bringing the nano-object to interrogate within the minuscule volume where the field is concentrated. There currently exists several approaches to control the immobilization of nano-objects but they all involve a cumbersome delivery step and require prior knowledge of the “hot spot” location.1−6 Herein, we present a novel technique in which the enhanced local field in the hot spot is the driving mechanism that triggers the binding of proteins via three-photon absorption. This way, we demonstrate exclusive immobilization of nanoscale amounts of bovine serum albumin molecules into the nanometer-sized gap of plasmonic dimers. The immobilized proteins can then act as a scaffold to subsequently attach an additional nanoscale object such as a molecule or a nanocrystal. This universal technique is envisioned to benefit a wide range of nano-optical functionalities including biosensing,7−12 enhanced spectroscopy like surface-enhanced Raman spectroscopy13,14 or surface-enhanced infrared absorption spectroscopy,15 as well as quantum optics.1,2,

The effect of dynamical scattering on single-plane phase retrieval in electron ptychography

Segmented and pixelated detectors on scanning transmission electron microscopes enable the complex specimen transmission function to be reconstructed. Imaging the transmission function is key to interpreting the electric and magnetic properties of the specimen, and as such four-dimensional scanning transmission electron microscopy (4D-STEM) imaging techniques are crucial for our understanding of functional materials. Many of the algorithms used in the reconstruction of the transmission function rely on the multiplicative approximation and the (weak) phase object approximation, which are not valid for many materials, particularly at high resolution. Herein, we study the breakdown of simple phase imaging in thicker samples. We demonstrate the behavior of integrated center of mass imaging, single-side band ptychography, and Wigner distribution deconvolution over a thickness series of simulated GaN 4D-STEM datasets. We further give guidance as to the optimal focal conditions for obtaining a more interpretable dataset using these algorithms

Development, assessment, and evolution of an interprofessional activity with senior nursing and pharmacy students: application of quality improvement in interprofessional education (IPE)

Purpose: Concurrent development IPE activities and an assessment strategy is essential for informed evolution of such initiatives. This study provides an active example of the progression of IPE at an institution utilizing an active assessment plan. Background: IPE is advocated as a method to develop students who are prepared for interprofessional practice, which is essential for optimal patient outcomes. While IPE has been integrated in many health professional curricula, focus on assessment is needed to ensure obtainment of educational outcomes and truly prepare students for interprofessional collaboration in the work place. Description of Intervention/Program: IPE activities were developed through a multidisciplinary committee consisting of health profession faculty and students. Additionally the committee developed an assessment strategy to evaluate IPE initiatives. One activity implemented over the last three years involves senior nursing and pharmacy students collaboratively addressing a complex patient care simulation. Students complete a pre/post survey evaluating the impact of the activity on their readiness for interprofessional practice, IPE perceptions, and activity impact. Group care plans and group evaluations are also evaluated. Results: Quantitative and qualitative data are reviewed by the committee annually to make informed decisions for future iterations of the activity. Conclusion: Execution of a clear assessment plan is essential to fulfill learning objectives for IPE activities. Feedback from an educational activity allowed for continuous improvement, ensuring impact on student learning, and illustrates the necessity of assessment within IPE. Relevance to IPE or Practice: The study conveys three key points that would be helpful to other institutions implementing IPE. First, the study illustrates the importance of assessment planning when implementing IPE activities. Second, the assessment plan presented serves as an example for others. Lastly, the study provides an active example of applying the assessment plan to iterations of an IPE activity. Seminar Outline/Timeframe of Presentation and Interactive Discussion: Opening discussion on IPE and assessment of IPE activities (10 minutes) Integration of IPE into the University curriculum and details regarding the senior IPE activity (10 minutes) Development of an IPE assessment plan and application to the senior IPE activity (10 minutes) Interprofessional groupwork: 5-6 multidisciplinary group members provided student feedback from an IPE activity and must determine potential adjustments to the activity for future iterations (10 minutes) Interprofessional groupwork: In the same group, discuss potential adjustments to the assessment plan for IPE activities for the activity discussed previously as well as at group members’ own institutions. Large group discussion of group work including information gleaned from student feedback, proposed changes for future activities, and ideas for assessment planning, both for the activity presented and for participants’ own institutions. Two to Three measureable learning objectives relevant to conference goals: To demonstrate successful development and application of assessment strategies in IPE activities. To guide use of student feedback in the improvement of IPE activities To stimulate discussion regarding assessment planning within IP

Interprofessional Education for Freshman Nursing and Pharmacy Students: An Application of Ethics

Purpose: The purpose is to encourage students in the health care professions to work interprofessionally to better enable them to enter the workplace as a member of the collaborative practice team. Background: In 2009, six national education associations of schools of the health professions formed a collaborative to promote and encourage constituent efforts that would advance substantive interprofessional learning experiences (IPE) to help prepare future health professionals for enhanced team-based care of patients and improved population health outcomes. By 2016, this initiative has grown significantly, even being mandated by some professions. Description of Intervention/Program: IPE activities were developed through a committee consisting of faculty and students from nursing and pharmacy programs at the institution. An activity for freshman students (N=152) was developed, focusing on each profession’s code of ethics and application to ethical situations. Students completed a pre/post survey evaluating their readiness and perceptions of IPE, as well as evaluating the activities’ effectiveness in effective collaboration using qualitative and quantitative techniques. Results: The data was evaluated by the IPE committee to determine future iterations of the activity. Conclusion: Based on both quantitative and qualitative feedback from the students, the freshman IPE activity assisted both pharmacy and nursing students to become a more effective member of the health care team, bringing students from different health care programs together to problem-solve while applying a collaboratively devised code of ethics with an application-based activity to produce a robust experience. Relevance to IPE or Practice: The study conveys two key points that would be helpful for others integrating IPE activities into their curriculum. First, the authors illustrated an IPE activity that can be implemented with lower level professional students. Second, the authors outlined a plan for continuous improvement of IPE activity, looking beyond implementation to assessment and optimization of these initiatives. Seminar Outline/Timeframe of Presentation and Interactive Discussion: Opening discussion on IPE and its integration into the university curriculum (10 minutes) Freshman activity initial ideas, design, and feedback: the value of interprofessional ethics in healthcare (10 minutes) Modifications to the freshman activity and student feedback ( 10 minutes) Interprofessional groupwork (5-6 group members made up of participants from different disciplines) to develop a shared code of ethics (10 minutes) Interprofessional groupwork (same group members) using the shared code of ethics to solve a practical, real-world problem (10 minutes) Large group discussion on the overall activity, components of the shared code of ethics, and application of the code to the problem-solving activity, and considerations for the future for this activity (10 minutes) Two to three measureable learning objectives relevant to conference goals: To create a coordinated effort across the nursing and pharmacy health profession curricula to embed essential interprofessional experience and content. To guide professional and institutional curricular development of learning approaches and assessment strategies to achieve productive outcomes for nursing and pharmacy students. To demonstrate a newly developed ethics activity for freshman students in nursing and pharmacy programs to exhibit interprofessional problem-solving

Development and Psychometric Evaluation of an Item Bank for Computerized Adaptive Testing of the EORTC Insomnia Dimension in Cancer Patients (EORTC CAT-SL)

To further advance assessment of patient-reported outcomes, the European Organisation of Research and Treatment of Cancer (EORTC) Quality of Life Group has developed computerized adaptive test (CAT) versions of all EORTC Quality of Life Core Questionnaire (QLQ-C30) scales/items. The aim of this study was to develop and evaluate an item bank for CAT measurement of insomnia (CAT-SL). In line with the EORTC guidelines, the developmental process comprised four phases: (I) defining the concept insomnia and literature search, (II) selection and formulation of new items, (III) pre-testing and (IV) field-testing, including psychometric analyses of the final item bank. In phase I, the literature search identified 155 items that were compatible with our conceptualisation of insomnia, including both quantity and quality of sleep. In phase II, following a multistep-approach, this number was reduced to 15 candidate items. Pre-testing of these items in cancer patients (phase III) resulted in an item list of 14 items, which were field-tested among 1094 patients in phase IV. Psychometric evaluations showed that eight items could be retained in a unidimensional model. The final item bank yielded greater measurement precision than the original QLQ-C30 insomnia item. It was estimated that administering two or more items from the insomnia item bank with CAT results in a saving in sample size between approximately 15–25%. The 8-item EORTC CAT-SL item bank facilitates precise and efficient measurement of insomnia as part of the EORTC CAT system of health-related quality life assessment in both clinical research and practice

Towards a critical epidemiology approach for applied sexual health research

Critical approaches may benefit epidemiological studies of sexual health. This article proposes a critical approach, reconcilable with social epidemiological enquiry. Key aims of critical epidemiology for sexual health are identified, from which three criticisms of practice emerge: (1) lack of attention to socio-cultural contexts, (2) construction of 'risk' as residing in the individual and (3) enactment of public health agendas which privilege and pathologise certain behaviours. These reflect and construct an apolitical understanding of population health. This article proposes features of a critical epidemiology that represent a morally driven re-envisioning of the focus, analysis and interpretation of epidemiological studies of sexual health

State based model of long-term potentiation and synaptic tagging and capture

Recent data indicate that plasticity protocols have not only synapse-specific but also more widespread effects. In particular, in synaptic tagging and capture (STC), tagged synapses can capture plasticity-related proteins, synthesized in response to strong stimulation of other synapses. This leads to long-lasting modification of only weakly stimulated synapses. Here we present a biophysical model of synaptic plasticity in the hippocampus that incorporates several key results from experiments on STC. The model specifies a set of physical states in which a synapse can exist, together with transition rates that are affected by high- and low-frequency stimulation protocols. In contrast to most standard plasticity models, the model exhibits both early- and late-phase LTP/D, de-potentiation, and STC. As such, it provides a useful starting point for further theoretical work on the role of STC in learning and memory

Effect of CGRP and sumatriptan on the BOLD response in visual cortex

To test the hypothesis that calcitonin gene-related peptide (CGRP) modulates brain activity, we investigated the effect of intravenous CGRP on brain activity in response to a visual stimulus. In addition, we examined if possible alteration in brain activity was reversed by the anti-migraine drug sumatriptan. Eighteen healthy volunteers were randomly allocated to receive CGRP infusion (1.5 μg/min for 20 min) or placebo. In vivo activity in the visual cortex was recorded before, during and after infusion and after 6 mg subcutaneous sumatriptan by functional magnetic resonance imaging (3 T). 77% of the participants reported headache after CGRP. We found no changes in brain activity after CGRP (P = 0.12) or after placebo (P = 0.41). Sumatriptan did not affect brain activity after CGRP (P = 0.71) or after placebo (P = 0.98). Systemic CGRP or sumatriptan has no direct effects on the BOLD activity in visual cortex. This suggests that in healthy volunteers both CGRP and sumatriptan may exert their actions outside of the blood–brain barrier

Metal-macrofauna interactions determine microbial community structure and function in copper contaminated sediments

Peer reviewedPublisher PD

Combining Classical and Molecular Approaches Elaborates on the Complexity of Mechanisms Underpinning Anterior Regeneration

The current model of planarian anterior regeneration evokes the establishment of low levels of Wnt signalling at anterior wounds, promoting anterior polarity and subsequent elaboration of anterior fate through the action of the TALE class homeodomain PREP. The classical observation that decapitations positioned anteriorly will regenerate heads more rapidly than posteriorly positioned decapitations was among the first to lead to the proposal of gradients along an anteroposterior (AP) axis in a developmental context. An explicit understanding of this phenomenon is not included in the current model of anterior regeneration. This raises the question what the underlying molecular and cellular basis of this temporal gradient is, whether it can be explained by current models and whether understanding the gradient will shed light on regenerative events. Differences in anterior regeneration rate are established very early after amputation and this gradient is dependent on the activity of Hedgehog (Hh) signalling. Animals induced to produce two tails by either Smed-APC-1(RNAi) or Smed-ptc(RNAi) lose anterior fate but form previously described ectopic anterior brain structures. Later these animals form peri-pharyngeal brain structures, which in Smed-ptc(RNAi) grow out of the body establishing a new A/P axis. Combining double amputation and hydroxyurea treatment with RNAi experiments indicates that early ectopic brain structures are formed by uncommitted stem cells that have progressed through S-phase of the cell cycle at the time of amputation. Our results elaborate on the current simplistic model of both AP axis and brain regeneration. We find evidence of a gradient of hedgehog signalling that promotes posterior fate and temporarily inhibits anterior regeneration. Our data supports a model for anterior brain regeneration with distinct early and later phases of regeneration. Together these insights start to delineate the interplay between discrete existing, new, and then later homeostatic signals in AP axis regeneration