Inibidores de PCSK-9: abordagens terapêuticas hipolipemiantes inovadoras em pacientes com síndrome metabólica

A patologia arterial coronariana exerce uma função primordial no desenvolvimento de doenças cardíacas isquêmicas, que englobam a angina e o infarto do miocárdio. Essas afecções lideram a prevalência e a letalidade globalmente, a dislipidemia um dos principais fatores de risco correlacionados com tais enfermidades. Neste contexto, a compreensão da fisiopatologia da arteriosclerose e o manejo das dislipidemias tornam-se imprescindíveis na prevenção e no tratamento da patologia arterial coronariana. Diante disso, o presente estudo objetivou ressaltar a relevância da utilização dos inibidores da enzima Pcsk9 na reversão do quadro dislipidêmico e na melhoria progressiva da patologia arterial coronariana. Observou-se a importância destes agentes farmacológicos em pacientes com elevado risco cardiovascular que não obtiveram resultados satisfatórios com o uso das principais classes terapêuticas indicadas para o controle do perfil lipídico. Quanto à metodologia empregada neste estudo, optou-se por realizar uma revisão da literatura, com o propósito de fornecer uma análise detalhada dos inibidores de PCSK9 - uma classe de proteases que contribuem para a redução dos receptores hepáticos de LDL, configura-se como uma abordagem terapêutica inovadora e promissora. Esse processo foi estruturado em diferentes etapas, a primeira fase dedicada à revisão bibliográfica, a fim de contextualizar amplamente o panorama do problema em questão

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)1.

In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field

Direito e cientificidade: experiência científica aplicada a Uniflu-FDC, aos contextos aprendentes e interativos de auto-realização nos cursos de graduação, lato-sensu e mestrado : tecnologias sócio-educativas por infovia

Trata das práticas tecnológicas sócioeducativas e os mecanismos que facilitam e viabilizam a produção de trabalhos científicos na Faculdade de Direito de Campos, tendo em vista a erradicação das deficiências nos conhecimentos básicos de informática, em informações técnicas de biblioteconomia e do idioma com redação científica

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)

In 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field

Guidelines for the use and interpretation of assays for monitoring autophagy (4th edition)

International audienceIn 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field