1,128 research outputs found
Housing Activism and Urban Space during the Covid19 Pandemic. Research Notes on the Bairro of Arroios, Lisbon
This paper presents some research notes from an on-going project on housing activism in Lisbon in the last decade, describing its ascendant trajectory (2012-2019) and the impact that the Covid epidemic had on the local activist community (2020-2022). In particular, the paper focuses on two of the main protagonists of local housing activism, the association Habita and the collective Stop Despejos, and on the relation that they have developed in time with an ecosystem (of sites, groups, projects) that have developed in the last ten years in the neighbourhood of Arroios, which have found a characteristic spatial infrastructure in the coletividades (a Portuguese expression that identifies spaces managed by no-profit associations or collectives). The paper examines this relation against the background of two bodies of literature, namely contributions that have examined (i) the nexus between collective action and space and (ii) the different forms of political agency represented by the conceptual pole of "contentious" and "everyday politics". This research is based on extensive data collection (through ethnographic notes, documental analysis, and in-depth interviews, 2020-2022) and on the authors' status of insiders in the process observed
Evaluating the Carbon Footprint of Cement Plants Integrated With the Calcium Looping CO2 Capture Process
Cement industry is estimated to account for∼6–7% of anthropogenic CO2emissionsglobally. Therefore, the identification of innovative solutions for their mitigation is both a priority and a challenge. The integration of carbon capture and storage technologies into the industrial production process is considered among the most viable solutions for this purpose, and calcium looping (CaL) represents one of the most promising. A key research challenge points to maximize process efficiencies and minimize production cost to decouple cement production from carbon emissions. The carbon capture process proposed in this work is a looping system where CO2 is absorbed by calcium oxide(CaO) in the first reactor (carbonator) and the calcium carbonate (CaCO3) produced is regenerated in an oxy-fired calciner. During calcination, CO2 is released from the sorbents, purified, compressed, and then made available for geological storage. In this study, greenhouse gas (GHG) emissions related to two cement production systems with CaL carbon capture are evaluated: the tail-end CaL carbon capture and the integratedCaL carbon capture. The carbon footprint is complemented with the assessment of the resources depletion mineral and elements and the demand of primary energy. An eco-design approach was pursued by carrying out a life cycle assessment to identify the environmental hotspots and which CaL integration approach presents a higher potential for cement industry decarbonization. The results of the analysis were compared with a conventional cement production process. The results show that the GHG emissions may be reduced by 74% with a tail-end approach and 71% when theCaL is fully integrated into the cement production process. When a future perspective, with higher penetration of renewable energy resources into the electricity sector, was modeled, the results showed that CaL integrated into the clinker production process is more promising in terms of reduction of the carbon footprint, rather than the tail-end solutions. Primary energy consumption from non-renewables is substantially impacted by CaL, with the integrated CaL configuration showing to be a more efficient solution because of less primary energy consumption (coal)
Default Mode Network alterations in alexithymia: An EEG power spectra and connectivity study
Recent neuroimaging studies have shown that alexithymia is characterized by functional alterations in different brain areas [e.g., posterior cingulate cortex (PCC)], during emotional/social tasks. However, only few data are available about alexithymic cortical networking features during resting state (RS). We have investigated the modifications of electroencephalographic (EEG) power spectra and EEG functional connectivity in the default mode network (DMN) in subjects with alexithymia. Eighteen subjects with alexithymia and eighteen subjects without alexithymia matched for age and gender were enrolled. EEG was recorded during 5 min of RS. EEG analyses were conducted by means of the exact Low Resolution Electric Tomography software (eLORETA). Compared to controls, alexithymic subjects showed a decrease of alpha power in the right PCC. In the connectivity analysis, compared to controls, alexithymic subjects showed a decrease of alpha connectivity between: (i) right anterior cingulate cortex and right PCC, (ii) right frontal lobe and right PCC, and (iii) right parietal lobe and right temporal lobe. Finally, mediation models showed that the association between alexithymia and EEG connectivity values was directed and was not mediated by psychopathology severity. Taken together, our results could reflect the neurophysiological substrate of some core features of alexithymia, such as the impairment in emotional awareness
Bud extracts from Tilia tomentosa Moench inhibit hippocampal neuronal firing through GABAA and benzodiazepine receptors activation
Simultaneous multisite detection of quantal release from PC12 cells using micro graphitic-diamond multi electrode arrays
Leptin counteracts the hypoxia-induced inhibition of spontaneously firing hippocampal neurons: a microelectrode array study.
Besides regulating energy balance and reducing body-weight, the adipokine leptin has been recently shown to be neuroprotective and antiapoptotic by promoting neuronal survival after excitotoxic and oxidative insults. Here, we investigated the firing properties of mouse hippocampal neurons and the effects of leptin pretreatment on hypoxic damage (2 hours, 3% O(2)). Experiments were carried out by means of the microelectrode array (MEA) technology, monitoring hippocampal neurons activity from 11 to 18 days in vitro (DIV). Under normoxic conditions, hippocampal neurons were spontaneously firing, either with prevailing isolated and randomly distributed spikes (11 DIV), or with patterns characterized by synchronized bursts (18 DIV). Exposure to hypoxia severely impaired the spontaneous activity of hippocampal neurons, reducing their firing frequency by 54% and 69%, at 11 and 18 DIV respectively, and synchronized their firing activity. Pretreatment with 50 nM leptin reduced the firing frequency of normoxic neurons and contrasted the hypoxia-induced depressive action, either by limiting the firing frequency reduction (at both ages) or by increasing it to 126% (in younger neurons). In order to find out whether leptin exerts its effect by activating large conductance Ca(2+)-activated K(+) channels (BK), as shown on rat hippocampal neurons, we applied the BK channel blocker paxilline (1 µM). Our data show that paxilline reversed the effects of leptin, both on normoxic and hypoxic neurons, suggesting that the adipokine counteracts hypoxia through BK channels activation in mouse hippocampal neurons
The effect of CdSe-ZnS quantum dots on calcium currents and catecholamine secretion in mouse chromaffin cells
Two firing modes and well-resolved Na+, K+, and Ca2+ currents at the cell-microelectrode junction of spontaneously active rat chromaffin cell on MEAs
We recorded spontaneous extracellular action potentials (eAPs) from rat chromaffin cells (CCs) at 37 °C using microelectrode arrays (MEAs) and compared them with intracellularly recorded APs (iAPs) through conventional patch clamp recordings at 22 °C. We show the existence of two distinct firing modes on MEAs: a ~ 4 Hz irregular continuous firing and a frequent intermittent firing mode where periods of high-intraburst frequency (~ 8 Hz) of ~ 7 s duration are interrupted by silent periods of ~ 12 s. eAPs occurred either as negative- or positive-going signals depending on the contact between cell and microelectrode: either predominantly controlled by junction-membrane ion channels (negative-going) or capacitive/ohmic coupling (positive-going). Negative-going eAPs were found to represent the trajectory of the Na(+), Ca(2+), and K(+) currents passing through the cell area in tight contact with the microelectrode during an AP (point-contact junction). The inward Nav component of eAPs was blocked by TTX in a dose-dependent manner (IC(50) ~ 10 nM) while the outward component was strongly attenuated by the BK channel blocker paxilline (200 nM) or TEA (5 mM). The SK channel blocker apamin (200 nM) had no effect on eAPs. Inward Nav and Cav currents were well-resolved after block of Kv and BK channels or in cells showing no evident outward K(+) currents. Unexpectedly, on the same type of cells, we could also resolve inward L-type currents after adding nifedipine (3 μM). In conclusion, MEAs provide a direct way to record different firing modes of rat CCs and to estimate the Na(+), Ca(2+), and K(+) currents that sustain cell firing and spontaneous catecholamines secretion
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