Corrélation phénotype-génotype dans la maladie de Rendu-Osler chez les patients du Poitou-Charentes

POITIERS-BU Médecine pharmacie (861942103) / SudocSudocFranceF

Valeur pronostique d un gradient distal d oxymétrie transcutanée dans la prise en charge des patients en ischémie critique non revascularisable

L oxygène est fondamental à la cicatrisation des ulcères. Par ailleurs, l oxymétrie transcutanée mesure de façon fiable, simple et sans danger la pression transcutanée en oxygène (TcPO2). Dans le domaine des valeurs basses de TcPO2 retrouvées dans l ischémie critique chronique des membres inférieurs (ICMI), les manoeuvres de sensibilisation permettent de préciser la mesure. Cette étude vise à analyser la valeur prédictive de l oxymétrie transcutanée distale avec sensibilisation dans la prise en charge de patients en ICMI non revascularisable et les effets thérapeutiques de l inhalation d oxygène. L étude a porté sur 16 patients sur une durée moyenne de 5 mois et demi. Il en ressort que l inhalation d oxygène est bénéfique au patient et que la TcPO2 avant sensibilisation, le gradient de TcPO2 issu de la sensibilisation et, pour les patients se situant sous les seuils fixés de ces 2 données, le facteur de multiplication de cette TcPO2 ont une valeur prédictive pronostique satisfaisante.NANTES-BU Médecine pharmacie (441092101) / SudocPARIS-BIUM (751062103) / SudocSudocFranceF

Prevalence and Recovery From Olfactory and Gustatory Dysfunctions in Covid-19 Infection: A Prospective Multicenter Study

International audienceBackground Covid-19 is defined by an association of multiple symptoms, including frequently reported olfactory and gustatory disorders. Objective The main purpose of this study was to analyze the prevalence of these neurosensory impairments in patients with Covid-19, and to assess short-term recovery. Methods We performed a multicenter case series study during the Covid-19 epidemic. All patients presenting a RT-PCR-confirmed SARS-CoV-2 infection were included, whether hospitalized or treated at home. To analyze the prevalence and features of olfactory and gustatory dysfunctions, a phone interview was conducted 5 days after the positive PCR result. The questionnaire was submitted again 10 days later to patients having reported olfactory and gustatory disorders, in order to assess their recovery. Results 115 patients were included in our study. 81 patients (70%) reported olfactory and gustatory disorders without nasal obstruction or rhinorrhea. These impairments were more frequently reported in the female population, young people, and house-bound patients with mild symptomatic forms. Short-term recovery assessed at Day 15 was complete for 64% of the patients, and incomplete in 33%. Median recovery time was 15 days (4–27 days) after olfactory or gustatory symptom onset. Conclusion Olfactory and gustatory dysfunctions related to Covid-19 are frequently reported and prevalent in mild symptomatic forms of the disease. Recovery in most cases seems rapid and complete

Multicenter evaluation of the Toxoplasma gondii Real-TM (Sacace) kit performance for the molecular diagnosis of toxoplasmosis

International audienceABSTRACT The molecular detection of Toxoplasma gondii DNA is a key tool for the diagnosis of disseminated and congenital toxoplasmosis. This multicentric study from the Molecular Biology Pole of the French National Reference Center for toxoplasmosis aimed to evaluate Toxoplasma gondii Real-TM PCR kit (Sacace). The study compared the analytical and clinical performances of this PCR assay with the reference PCRs used in proficient laboratories. PCR efficiencies varied from 90% to 112%; linearity zone extended over four log units (R 2 > 0.99) and limit of detection varied from 0.01 to ≤1 Tg/mL depending on the center. Determined on 173 cryopreserved DNAs from a large range of clinical specimens, clinical sensitivity was 100% [106/106; 95 confidence interval (CI): 96.5%–100%] and specificity was 100% (67/67; 95 CI: 94.6%–100%). The study revealed two potential limitations of the Sacace PCR assay: the first was the inconsistency of the internal control (IC) when added to the PCR mixture. This point was not found under routine conditions when the IC was added during the extraction step. The second is a lack of practicality, as the mixture is distributed over several vials, requiring numerous pipetting operations. Overall, this study provides useful information for the molecular diagnosis of toxoplasmosis; the analytical and clinical performances of the Sacace PCR kit were satisfactory, the kit having sensitivity and specificity similar to those of expert center methods and being able to detect low parasite loads, at levels where multiplicative analysis gives inconsistently positive results. Finally, the study recommends multiplicative analysis in particular for amniotic fluids, aqueous humor, and other single specimens