Comparing water relations and stomatal regulation of Touriga Nacional and Syrah under mild water stress

Proceedings - IX International Terroir Congress, 2012Aiming to compare the physiological responses of the Portuguese red variety Touriga Nacional (TN) with Syrah (SY), we studied during the 2007 growing season five-year-old grapevines growing in a commercial non-irrigated vineyard located at the Lisbon winegrowing region. Predawn (Ypd) and midday (YM) leaf water potential, leaf stomatal conductance (gs) and photosynthetic rate (A) were periodically measured between fruit set and harvest. Ypd displayed a decreasing pattern throughout the growing cycle from -0.10 MPa at flowering to -0.44 MPa at harvest. Both varieties showed similar values except during the two measurements made in August when TN presented significantly higher values than SY. Ymid also showed a decreasing pattern from the end of June towards harvest date, with significant differences between varieties being observed during the ripening period, with SY showing lower values than TN. A measured either at mid-morning and midday presented, for most part of the cases, lower values in SY than in TN, although the differences were only significant during the ripening period. gs pattern was parallel to A and, in general, the relative differences between varieties mirrored those reported for A. No significant effect of the variety was detected on the relationships between A or gs measured at mid-morning and Ypd. However, when analyzing the set of data collected at midday it was observed that the regression lines of the relationships between A or gs (dependent variables) and Ypd (independent variable) presented a significantly higher slope in SY as compared to those showed by TN. These results show that the rate of decrease of A and gs with the decrease of Ypd was lower in TN than in SY suggesting that the two varieties have different stomatal regulation, with a more “optimistic” behavior in TN

Micromorfoanatomia foliar de cultivares tintas de Vitis vinifera SSP.vinifera (Vitaceae)

Aiming to characterize and discriminate between four red grapevine cultivars – ‘Aragonez’ (AR), ‘Cabernet Sauvignon’ (CS), ‘Syrah’ (SY) and ‘Touriga Nacional’ (TN) – grown under Mediterranean field conditions, we studied their leaf micromorphoanatomic characteristics under light (LM) and scanning electron microscopy (SEM). The studied characteristics included those of the epidermis, stomata and hair distribution, and the mesophyll structure. The individual primary leaf area revealed significant differences between cultivars, with the highest value presented by AR and the lowest by CS, while SY and TN gave intermediate values. CS presented a significantly higher leaf specific dry weight value than the other three cultivars, which returned similar values. Under SEM magnification three types of stomata were identified in all the studied genotypes: sunken, at the same level, and raised above the other epidermal cells. Each cultivar displayed different percentages of these types of stomata: the highest raised-above values were observed in AR; TN had the highest same-level values and the lowest sunken ones; CS revealed the highest values for sunken stomata; while SY returned average values for all the types of stomata. Stomatal density was higher in AR and SY and lower in CS and TN. The hairs on the lower surface presented a similar woolly aspect in all the studied cultivars, but the mesophyll structure was quite different: CS presented the highest and AR the lowest values for total thickness of the lamina, thickness of palisade and spongy parenchyma, and length and thickness of upper and lower epidermal cells; the values for these leaf features in TN and SY fell between those for CS and AR. The data suggest that differences in leaf micromorphoanatomy can be used to distinguish between grapevine cultivars. Further studies are needed to confirm whether there is any association between some of these leaf traits – e.g. stomata type and mesophyll structure – and the physiological behaviour observed under field conditions

Leaf morphoanatomy of four red grapevine cultivars grown under the same terroir

XII Congreso Internacional TerroirThis study compare leaf morphoanatomical characteristics of four red cultivars - ‘Touriga Nacional’, ‘Trindadeira’, ‘Cabernet Sauvignon’ and ‘Syrah’ -, grown side by side at the same terroir. The analyzed leaf traits, under light and scanning electron microscopy, showed large variability among genotypes. ‘Trincadeira’ has the biggest single leaf area and ‘Cabernet Sauvignon’ the smallest one. ‘Touriga Nacional’ showed the lowest leaf dry weight and ‘Trincadeira’ the highest one, nonetheless there was no significantly differences in leaf dry mass per area and in leaf density. Leaf dry mass per area was positively correlated with leaf density but showed no correlation with leaf thickness. The French genotypes presented higher thickness of the leaf anatomical traits than the two Portuguese ones. ‘Trincadeira’ showed significantly highest stomata density while the other cultivars showed no significant differences among them. The analyses of the three types of stomata revealed that ‘Trincadeira’ has the lower percentage of raised above and the highest percentage of sunken stomata while ‘Cabernet Sauvignon’ showed the opposite behaviour. The hairs on the lower surface presented a similar woolly aspect in all cultivars. The possible role of leaf morphoanatomical characteristics in determining the cultivars adaptation to abiotic stresses is suggested and discussedinfo:eu-repo/semantics/publishedVersio

Dinâmica da vegetação infestante das vinhas da Região Vitivinicola do Dão

With the aim of analysing the influence of both the sub-region and weed-control practices on vegetation composition and dynamics in the Portuguese Dão Winegrowing Region, 120 vineyards were surveyed for weeds and winegrowers were asked to respond to a questionnaire in 2008. 206 taxa distributed across 31 families were recorded, with a predominance of Fabaceae, Asteraceae and Poaceae. The predominant vineyard inter-row soil management techniques were cover cropping (53%), with some cover sown but mostly relying on resident vegetation, and soil tillage (31%). Herbicides were applied in the row in 46%, and in the overall area in 4%, of the vineyards. Canonical correspondence analysis revealed signifi cant effects of the sub-region and the different soil management strategies on vineyard community structure. Compared with the last (and only) study, which took place in 1950, weed composition was different and species diversity increased. The present study showed that resident vegetation also increased plant diversity. The dynamics of the perennial weed species Cynodon dactylon and Convolvulus arvensis must be carefully evaluated in order to avoid severe competition with the vine

Caracteristicas morfoanatómicas da folha para a determinação da capacidade de adaptação da videira aos stresses abióticos: revisão

ReviewWorldwide, there are thousands of Vitis vinifera grape cultivars used for wine production, creating a large morphological, anatomical, physiological and molecular diversity that needs to be further characterised and explored, with a focus on their capacity to withstand biotic and abiotic stresses. This knowledge can then be used to select better adapted genotypes in order to help face the challenges of the expected climate changes in the near future. It will also assist grape growers in choosing the most suitable cultivar(s) for each terroir; with adaptation to drought and heat stresses being a fundamental characteristic. The leaf blade of grapevines is the most exposed organ to abiotic stresses, therefore its study regarding the tolerance to water and heat stress is becoming particularly important, mainly in Mediterranean viticulture. This review focuses on grapevine leaf morphoanatomy - leaf blade form, leaf epidermis characteristics (cuticle, indumentum, pavement cells and stomata) and anatomy of mesophyll - and their adaptation to abiotic stresses. V. vinifera xylem architecture and its adaptation capacity when the grapevine is subjected to water stress is also highlighted since grapevines have been observed to exhibit a large variability in responses to water availability. The hydraulic properties of the petiole, shoot and trunk are also reviewed. Summarising, this paper reviews recent advances related to the adaptation of grapevine leaf morphoanatomical features and hydraulic architecture to abiotic stresses, mainly water and heat stress, induced primarily by an ever-changing global climateinfo:eu-repo/semantics/publishedVersio

Anatomia da folha de cultivares brancas de videira com distinta origem geográfica

This study aims to compare the leaf morphoanatomical characteristics of seven Vitis vinifera subsp. vinifera white cultivars with different geographical origin, grown side by side at the same “terroir”. The leaf morphoanatomical traits analyzed under light and scanning electron microscopy (SEM) showed large variability among genotypes. Significant differences between cultivars were observed in single leaf area, specific leaf area, leaf density and in the thickness of cuticle, upper and lower epidermal cells and mesophyll. Leaf dry mass per area presented a significant positive correlation with leaf density but showed no correlation with leaf thickness, results that can be explained by the quite different mesophyll structure, epidermal cells and cuticle thickness. ‘Alvarinho’, ‘Encruzado’, ‘Macabeu’ and ‘Viosinho’ were the cultivars with the highest thickness of cuticle and mesophyll tissues. Under SEM magnification three types of stomata were identified: sunken, at the same level and raised above, with the same level type presenting the higher percentage in all cultivars. Stomata density presented significant differences between cultivars, with ‘Macabeu’ showing the highest value and ‘Alvarinho’ and ‘Arinto’ the lowest ones. The hairs on the lower surface presented a similar woolly aspect in all cultivars. Calcium oxalate crystals, raphids and druses were visible and widely distributed in the parenchyma tissues. The observed differences in leaf traits among genotypes suggest a major role of leaf anatomy in determining grapevine capacity for coping with different environmental conditionsinfo:eu-repo/semantics/publishedVersio

Tecnologia Vitícola para Vinhos de Qualidade

info:eu-repo/semantics/publishedVersio

Enrelvamento da Vinha

info:eu-repo/semantics/publishedVersio

Enrelvamento em vinha de encosta não regada: 1 - efeito na composição e dinâmica das infestantes

The influence of two sward treatments and soil cultivation on the composition, the structure, and the evolution of the biomass of vineyard weed communities was examined. The 3-year study (2002-2004) was carried out in a sloping, non-irrigated vineyard, cv. ‘Cabernet Sauvignon’, in the Estremadura winegrowing region of Portugal. The experimental treatments were: soil tillage (control); permanent sown cover crop - Lolium perenne ‘Nui’, L. multiflorum ‘Bartíssimo’, Festuca ovina ‘Ridu’, F. rubra ssp. rubra ‘Echo’, Trifolium incarnatum. ‘Red’, T. repens ‘Huie’ and T. subterraneum ‘Claire’; and permanent resident vegetation. Total weed biomass in the spring did not reveal significant differences between treatments, but varied annually. The management practices – e.g. time and number of soil cultivations and inter-row mowing – were determinant in weed biomass evolution. Canonical correspondence analysis revealed significant treatment effects on community structure. Three years after the experiment was set up, in the soil tillage treatment weed composition was dominated by annual broad-leaved species, namely five Geraniaceae species, Medicago polymorpha and Sonchus oleraceus. The perennial broad-leaved species Oxalis pes-caprae was also a dominant species in soil tillage. In both sward treatments there was an increase in the perennial broad-leaved and grass species. Compared to soil tillage, in the resident vegetation treatment there was a significant increase in perennial species, such as Rumex crispus, Veronica anagallis-aquatica and Polypogon monspeliensis, and in the annuals Melilotus indica and Avena sterilis. The increase in these perennial species, which are considered to compete with vines, requires more frequent mowing in the summer. In the permanent sown cover crop treatment, L. perenne and T. repens displayed the ability to re-establish successfully, and their abundance decreased or suppressed most of the annual and perennial weed species

Enrelvamento em vinha de encosta não regada: 2 - efeitos no crescimento vegetativo, produção e qualidade do mosto e vinho, casta Cabernet Sauvignon

Grapevine vegetative growth, yield, fruit composition and wine quality were studied in the Estremadura Winegrowing Region of Portugal in a ‘Cabernet Sauvignon’ slopping non-irrigated vineyard. During three seasons three treatments were compared: soil tillage (control), permanent resident vegetation, and permanent sown cover crop. When compared to soil tillage, the inter-row sward treatments displayed a lower predawn leaf water potential from bloom to mid-ripening. These differences in vine water status did not affect vine yield or berry sugar accumulation; however, in the third season after experiment setup it induced a significant reduction in vegetative growth in the sward treatments, compared to soil tillage. This vegetative growth reduction had a positive effect on grape composition by reducing titratable acidity and increasing berry skin total phenols and anthocyanins. Those differences were also detected in the wines by the judges who gave a better classification to the wines from the sward treatments. Our results indicate that cover cropping can be a valuable tool for controlling vigour and enhancing wine quality in this winegrowing region